Evaluation of paracoagulation tests by plasma fibrinogen chromatography

Musumeci, V; Marra, R; Zappacosta, Bruno; Carloni, Luca P.; Cristofari, C.

doi:10.1016/0049-3848(80)90300-x

Cited by 4 publications

(4 citation statements)

References 14 publications

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“…The observation that a plasma concentration of 24% did not yield a two fold greater increase in turbidity than a plasma concentration of 12% might be due to the fact that not only soluble fibrin is diluted but all plasma proteins,· and albumin för example is said to have an inhibitory effect on protamine sulphate. A plasma fraction of 0.20 in the test provided better results than concentrations of 50 to 90% äs required for visual estimations by other authors (11,12,(24)(25)(26)). …”

Section: Kineticsmentioning

confidence: 93%

“…Several other authors have used protamine sulphate concentrations up to 2 g/l (14,25), and the effect of such concentrations on fibrinogen precipitation has been discussed controversially (10,11,15,24,29). Gurewich also used final protamine sulphate concentrations of 0.2 g/l or less in order to avoid fibrinogen precipitation (11).…”

Section: Kineticsmentioning

confidence: 99%

“…The initial turbidity of the plasma sample does not affect the results during kinetic measurements. Earlier studies used endpoint measurements after 5 -10 minutes or visual estimations 15 -30 minutes after addition of protamine sulphate (11,12,(24)(25)(26). In some studies additional controls were performed after 24 hours, in order to avoid false positive results through precipitation of fibrinogen (11,13).…”

Section: Kineticsmentioning

confidence: 99%

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Determination of Soluble Fibrin by Turbidimetry of its Protamine Sulphate-Induced Paracoagulation

Wieding¹,

Eisinger²,

Köstering³

1989

Clinical Chemistry and Laboratory Medicine

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Summary: Protamine sulphate-induced aggregation of soluble fibrin causes an increase of turbidity in the plasma sample, which can be measured by means of kinetic turbidimetry.A method was developed which is sufficiently sensitive for the determination of soluble fibrin in plasma without iüterfering with the sensitivity for fibrinogen. The performance of the assay was studied by analysing plasma samples with high concentrations of fibrinogen and soluble fibrin at different pH values, at different concentrations of plasma and protamine sulphate, and using different wavelengths and analysis times. Measurement of thrombin-induced fibrinogen-fibrin-transformation by the developed turbidimetric method, gave results that correlated well with the release of fibrinopeptide A.The new protamine sulphate method for turbidimetric dclcrmination of soluble fibrin is charactcrizcd by its practicability, rapid availability of reproducible, quantitative results, and ils economy ofreagenls and time in single and serial analysis. Therefore it seems well-suited for the routine diagnosis of hypercoagulability with increased fibrinogen turnover.

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Section: Kineticsmentioning

confidence: 93%

Section: Kineticsmentioning

confidence: 99%

Section: Kineticsmentioning

confidence: 99%

See 1 more Smart Citation

Determination of Soluble Fibrin by Turbidimetry of its Protamine Sulphate-Induced Paracoagulation

Wieding¹,

Eisinger²,

Köstering³

1989

Clinical Chemistry and Laboratory Medicine

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“…Einleitung rinnung (1,2 ergeben einen hohen Prozentsatz falsch negativer und falsch positiver Resultate (11). Eine empfindlichere und spezifischere Methode zum Nachweis von Fibrinmonomere ist der Erythrozyten-Agglutinations-Test nach Largo (12), der unter der Bezeichnung "FM-Test" von Boehringer Mannheim bezogen werden kann.…”

Section: Abstract: Fibrin Monomer -Antithrombin III -Fibrin Degradatiunclassified

Der Nachweis von löslichen Fibrinmonomer- Komplexen. Methodenvergleich eines Erythrozyten- Agglutinationstests nach Largo (FM-Test) mit dem Äthanol-Gelierungs-Test

Oehler¹,

Klaus²,

Stötzer³

et al. 1987

LaboratoriumsMedizin

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Hypercoagulability and disseminated intravascular coagulation (DIG) are characterized, by the presence of circulating fibrin monomer complexes in plasma. In 342 patients with possible DIC fibrin monomers, fibrinogen, repti/ase® time, antithrombin III and other coagulation parameters were determined at frequent intervals. Testing of soluble fibrin monomer complexes was performed using a sensitive and reliable haemagglutination assay with red cells sensitized by fibrin monomers (FM-Test) and the ethanol gelation test (EGT). Method comparison regarding the influence of fibrinogen levels and fibrin degradation products shows that high fibrinogen levels lead to false-positive results with EGT. The same effect is observed for fibrin degradation products and EGT whereas no influence of fibrinogen level and fibrin degradation products on the FM-Test occurs. It is wellknown that during DIC AT III level decreases caused by proteolytic activity.In this study it could be shown that fibrin monomer increases parallel to the decrease of AT III. The same effect does not occur due to fibrin degradation products.

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