Evaluation of pap and sfa Genes Relative Frequency P and S Fimbriae Encoding of Uropathogenic Escherichia coli Isolated from Hospitals and Medical Laboratories; Yasuj City, Southwest Iran

Boroumand, Mostafa; Sharifi, Asghar; Manzouri, Leila; Khoramrooz, Seyed Sajjad; Khosravani, Seyed Abdolmajid

doi:10.5812/ircmj.89499

Cited by 7 publications

(12 citation statements)

References 43 publications

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“…The sfa was the second most predominant adhesin gene (81%) in our isolates that was in agreement with a research carried out in Shiraz (Fars province, Iran) with a frequency of 79.4% (29). In previous reports, the prevalence of the sfa was conversely less than 30% (7). Nevertheless, the exact role of S-fimbriae is not clarified; therefore, the distribution of bacterium within the host tissue depends on this adhesin marker (29).…”

Section: Discussionsupporting

confidence: 91%

“…The most significant adhesin-encoding genes include fimH, sfa, papC, foc, afa, papGI, papGII, and papGIII and genes related to the adhesin systems. Several studies have shown that these adhesin factors have significant roles in the pathogenicity of UPEC strains because the strains are able to colonize and overcome host defense systems, thereby resulting in the disease (6,7). The increase in the multi-drug resistant strains of E. coli in many countries has caused failure in the treat-ment accompanied by a huge health burden (8).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Uropathogenic Escherichia coli: Distribution of Adhesin-Encoding Genes and O-Serotypes Among Ciprofloxacin Susceptible and Resistant Isolates

Rashki

Rahdar

Ghalehnoo

2019

Jundishapur J Microbiol

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Background: Some evidence indicates that there is a potential linkage between ciprofloxacin resistance and the prevalence of virulence factors in pathogenic Escherichia coli strains. Objectives: The study was conducted to evaluate the association of eight putative adhesin-encoding genes and 12 O-serotypes among ciprofloxacin susceptible/resistant in uropathogenic E. coli (UPEC) isolates. Methods: A total of 100 E. coli isolates collected from symptomatic patients with urinary tract infection were surveyed for antimicrobial susceptibility test and polymerase chain reaction (PCR) to find the presence of eight putative adhesin-encoding genes and 12 O-serotype and their association with ciprofloxacin susceptibility and resistance. Results: The highest and the lowest resistance rates were obtained against ampicillin (92%) and gentamycin (19%), respectively. However, the resistance rate to ciprofloxacin was detected in 43% of E. coli isolates. PCR results revealed the frequency of eight putative adhesin markers ranged from 4 to 95%. The presence of 2 genes (fimH and sfa) was >76% among all screened isolates. Of 100 UPEC isolates, 73 were positive for one of the tested O-serotypes. The most common types of O-serotype were O2 (16%), O6 (16%), and O18 (14%). The fimH, sfa, and papC were the most prevalent pattern in the ciprofloxacin-susceptible isolates (31 vs. 17). There was no significant correlation between the presence of O-serotype, adhesin-encoding genes and susceptibility/resistance to ciprofloxacin. Conclusions: The results revealed that UPEC isolates of different geographical regions might have various properties. It is worthwhile to elucidate the differences that might result in producing valuable evidence based on clinical guidelines for the management of urinary tract infection.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Characterization of Uropathogenic Escherichia coli: Distribution of Adhesin-Encoding Genes and O-Serotypes Among Ciprofloxacin Susceptible and Resistant Isolates

Rashki

Rahdar

Ghalehnoo

2019

Jundishapur J Microbiol

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“…Among them, Escherichia coli is the most common agent that can cause infections at different ages. Studies in different communities have shown that Gram-negative bacilli, especially E. coli, cause more than 80 -90% of infections (1,2). A group of researchers has emphasized that the type of E. coli phylogenetic group plays an important role in their pathogenicity (3)(4)(5).…”

Section: Introductionmentioning

confidence: 99%

Detection of Phylogenetic Groups and Drug Resistance Genes of Escherichia coli Causing Urinary Tract Infection in Southwest Iran

Boroumand

Naghmachi

Ghatee

2021

Jundishapur J Microbiol

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Background: Many bacteria can cause urinary tract infections (UTIs), among which Escherichia coli is the most common causative agent. E. coli strains are divided into eight phylogenetic groups based on the new Quadroplex-PCR method, which are different in terms of patterns of resistance to antibiotics, virulence, and environmental characteristics. Objectives: This study aimed to determine the phylogenetic groups and the prevalence of drug resistance genes in E. coli strains causing UTIs. Methods: In this descriptive cross-sectional study, 129 E. coli isolates obtained from the culture of patients with UTIs were evaluated for phylogenetic groups using the new method of Clermont et al. The identification of phylogenetic groups and antibiotic resistance genes was performed using the multiplex polymerase chain reaction (PCR) method. Results: In this study, concerning the distribution of phylogenetic groups among E. coli isolates, the phylogenetic group B2 (36.4%) was the most common phylogenetic group, followed by phylogroups C (13.2%), clade I (10.1%), D (9.3%), and A (3.1%) while groups B1 and F were not observed in any of the isolates, and 20.2% had an unknown state. Also, out of 129 E. coli isolates, the total frequency of tetA, tetB, sul1, sul2, CITM, DfrA, and qnr resistance genes was 59.7%, 66.7, 69, 62, 30.2, 23.3, and 20.2%, respectively. In this study, there was a significant relationship between antibiotics (P = 0.026), cefotaxime (P = 0.003), and nalidixic acid (P = 0.044) and E. coli phylogenetic groups. No significant relationship was observed between E. coli phylogenetic groups and antibiotic resistance genes. Conclusions: The results of this study showed that strains belonging to group B2 had the highest prevalence among other phylogroups, and also, the frequency of antibiotic resistance genes and drug-resistant isolates had a higher prevalence in this phylogroup. These results show that phylogroup B2 has a more effective role in causing urinary tract infections compared to other phylogroups, and this phylogroup can be considered a genetic reservoir of antibiotic resistance.

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“…The AMR test results showed that none of the isolates were resistant to less than 4 antimicrobials tested so far, although a high sensitivity was recorded to amikacin (80.6%). Studies by Giwa et al, (2018), and Boroumand et al, (2019) recorded similar results of isolates showing high sensitivity to amikacin [28,29]. Even among the aminoglycosides currently available for use in humans, the most resistant to the action of aminoglycoside modifying enzymes is amikacin [30].…”

Section: Discussionmentioning

confidence: 78%

“…The ability to colonize and produce biofilm by bacteria is facilitated by adhesive structures which, in addition, was able to restrict antibiotic penetration into the cells [32]. The production of biofilm in bacteria can serve as a means of protection from exposure to antimicrobials, when compared with non-biofilm producing bacteria [33], as well a factor that plays a key role in the development of hospital-acquired infections and urinary tract infections (UTIs) [29].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistant and virulence genes profiles of some Gram-negative bacteria from clinical isolates at Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria

Tanko¹,

Ong²,

Bolaji³

et al. 2020

J Pre Clin Clin Res.

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Introduction and objective. Infections due to multidrug-resistant (MDR) Enterobacteriaceae are an ongoing global threat in their management. The aim of the study was to investigate the antiimicrobial resistance (AMR) and virulence gene profiles of MDR Gram-negative isolates in Sokoto, northwest Nigeria. Materials and method. A total of 578 clinical samples were collected from patients. Suspected Gram-negative bacteria were isolated from these clinical samples: vaginal swab, pus, stool, blood, wound swab and urine, using Gram-staining and conventional biochemical reactions. These isolates were further identified with an identification kit (Microgen-GN-A), and tested against a panel of 11 antibiotics. A single polymerase chain reaction (PCR) assay targeting 13 virulence gene related to adhesion (fimH, papC, and sfaS), iron chelation (iutA, and fyuA), toxins (astA, stx1, stx2, and eaeA), biofilm (bssS), and serum resistance (traT, iss, and kapsMTII) encoding genes were evaluated. Results. A total of 276 Gram-negative isolates were identified using the Gram stain and biochemical reactions. These organisms were further confirmed with identification kit. Of the 276 isolates, 36 organisms of interest (23 Escherichia coli, 4 Klebsiella pneumoniae and 9 Proteus mirabilis) were identified. Other Gram-negative isolates accounted for the remaining 86.9%. The majority of the isolates were resistant to cefixime (100%) and partially resistant to amikacin (19.4%).The virulence genes bssS (58.3%), fimH (44.4%), and iutA (44.4%) were the most prevalent, whereas kapsMTII (5.6%) and stx2 (2.8%) were least detected, while astA was not detected in any of the isolates. Conclusion. The study elucidated the prevalence of antibiotic resistance and virulence genes in Gram-negative bacteria from clinical isolates in Sokoto, northwestern Nigeria. The majority of the isolates were MDR, thereby posing a public health risk.

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Evaluation of pap and sfa Genes Relative Frequency P and S Fimbriae Encoding of Uropathogenic Escherichia coli Isolated from Hospitals and Medical Laboratories; Yasuj City, Southwest Iran

Cited by 7 publications

References 43 publications

Characterization of Uropathogenic Escherichia coli: Distribution of Adhesin-Encoding Genes and O-Serotypes Among Ciprofloxacin Susceptible and Resistant Isolates

Characterization of Uropathogenic Escherichia coli: Distribution of Adhesin-Encoding Genes and O-Serotypes Among Ciprofloxacin Susceptible and Resistant Isolates

Detection of Phylogenetic Groups and Drug Resistance Genes of Escherichia coli Causing Urinary Tract Infection in Southwest Iran

Antimicrobial resistant and virulence genes profiles of some Gram-negative bacteria from clinical isolates at Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria

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