Evaluation of paclitaxel (taxol), cisplatin, and the combination paclitaxel-cisplatin in ovarian cancer in vitro with the ATP cell viability assay

Untch, Michael; Sevin, B.-U.; Perras, J.P.; Angioli, Roberto; Untch, Andrea; Hightower, Randall D.; Koechli, Ossi R.; Averette, H.E.

doi:10.1016/0020-7292(94)90607-6

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The visualized DNA fragmentation pattern shown at 2× Blue cheese was seemingly similar to the pattern demonstrated at 50 μM cisplatin. It is to be noted that the circulating levels of cisplatin in humans reach approximately 12.5 μM (Untch et al, 1994;Andreotti et al, 1995). It has been reported that bovine milk and its fermented products have potential cytotoxic properties against different types of tumor cells in vitro and murine breast cancer cells in vivo (Ito et al, 1998;Mader et al, 2005;de Moreno de LeBlanc et al, 2005).…”

Section: Apoptosis Induced By Highly Ripened Cheesesmentioning

confidence: 99%

Effects of highly ripened cheeses on HL-60 human leukemia cells: Antiproliferative activity and induction of apoptotic DNA damage

Yasuda¹,

Ohkura²,

Suzuki³

et al. 2010

Journal of Dairy Science

View full text Add to dashboard Cite

To establish cheese as a dairy product with health benefits, we examined the multifunctional role of cheeses. In this report, we clarify whether different types of commercial cheeses may possess antiproliferative activity using HL-60 human promyelocytic leukemia cell lines as a cancer model. Among 12 cheese extracts tested, 6 (Montagnard, Pont-l'Eveque, Brie, Camembert, Danablue, and Blue) revealed strong growth inhibition activity and induction of DNA fragmentation in HL-60 cells. Based on the quantification of nitrogen contents in different cheese samples, a positive correlation between the ripeness of various cheeses and their antiproliferative activity tested in HL-60 cells was displayed. Four varieties of Blue cheese ripened for 0, 1, 2, or 3 mo demonstrated that the Blue cheese ripened for a long term was capable of causing the strong suppression of the cell growth and the induction of apoptotic DNA damage as well as nucleic morphological change in HL-60 cells. Collectively, these results obtained suggest a potential role of highly ripened cheeses in the prevention of leukemic cell proliferation.

show abstract

Section: Apoptosis Induced By Highly Ripened Cheesesmentioning

confidence: 99%

Effects of highly ripened cheeses on HL-60 human leukemia cells: Antiproliferative activity and induction of apoptotic DNA damage

Yasuda¹,

Ohkura²,

Suzuki³

et al. 2010

Journal of Dairy Science

View full text Add to dashboard Cite

show abstract

“…Their costs should also been taken into consideration when screening large chemical libraries. There are several cytotoxic assay types that are commonly used by both academic researchers and the pharmaceutical industry; these include and are not limited to ATP quantification [2], XTT/MTT [3], LDH release [4], and glutathione depletion [5]. …”

Section: Introductionmentioning

confidence: 99%

A high density assay format for the detection of novel cytotoxic agents in large chemical libraries

Shum

Radu

Kim

et al. 2008

Journal of Enzyme Inhibition and Medicinal Chemistry

View full text Add to dashboard Cite

The Alamar Blue (AB) assay, which incorporates a redox indicator that causes a fluorescence signal enhancement in response to metabolic activity, is commonly used to assess the viability of mammalian cells. In response to the need for homogeneous, inexpensive, high throughput assays for anti-cancer drug screening, a 1536-well microtiter plate based assay which utilizes the AB fluorescent dye as a measure of cellular growth was developed and validated in 10 µL assay volume. The performance and robustness of the miniaturized assay was assessed using a human Mantle Cell Lymphoma (MCL) cell line in a pilot screen against a library of 2,000 known bioactive chemicals; with an overall Z’ value of 0.89 for assay robustness, several known cytotoxic agents were identified including and not limited to anthracyclines, cardiac glycosides, gamboges, and quinones. To further test the sensitivity of the assay, IC50 determinations were performed in both 384-well and 1536-well formats and the obtained results show a very good correlation between the two density formats. These findings demonstrate that this newly developed assay is simple to set up, robust, highly sensitive and inexpensive. The non-radiometric strategy employed in this study should also offer the potential for the rapid screening, without a wash or a lysis step, of well established and primary tumor cell lines against large chemical libraries using the 1536-well microtiter plates.

show abstract

Evaluation of paclitaxel (taxol), cisplatin, and the combination paclitaxel-cisplatin in ovarian cancer in vitro with the ATP cell viability assay

Cited by 2 publications

References 0 publications

Effects of highly ripened cheeses on HL-60 human leukemia cells: Antiproliferative activity and induction of apoptotic DNA damage

Effects of highly ripened cheeses on HL-60 human leukemia cells: Antiproliferative activity and induction of apoptotic DNA damage

A high density assay format for the detection of novel cytotoxic agents in large chemical libraries

Contact Info

Product

Resources

About