Effects of highly ripened cheeses on HL-60 human leukemia cells: Antiproliferative activity and induction of apoptotic DNA damage

Abstract

To establish cheese as a dairy product with health benefits, we examined the multifunctional role of cheeses. In this report, we clarify whether different types of commercial cheeses may possess antiproliferative activity using HL-60 human promyelocytic leukemia cell lines as a cancer model. Among 12 cheese extracts tested, 6 (Montagnard, Pont-l'Eveque, Brie, Camembert, Danablue, and Blue) revealed strong growth inhibition activity and induction of DNA fragmentation in HL-60 cells. Based on the quantification of nitrogen contents in different cheese samples, a positive correlation between the ripeness of various cheeses and their antiproliferative activity tested in HL-60 cells was displayed. Four varieties of Blue cheese ripened for 0, 1, 2, or 3 mo demonstrated that the Blue cheese ripened for a long term was capable of causing the strong suppression of the cell growth and the induction of apoptotic DNA damage as well as nucleic morphological change in HL-60 cells. Collectively, these results obtained suggest a potential role of highly ripened cheeses in the prevention of leukemic cell proliferation.

Evaluation of anti‐proliferative activity of Cheddar cheeses using colon adenocarcinoma (HCT‐116) cell line

“…In our study, the extracts derived from CCC have shown relatively more potent anti‐proliferative activity towards colon cancer. Similarly, Yasuda et al () also demonstrated the anti‐proliferative potential and apoptosis induction by ripened cow milk cheeses. When cells undergo apoptosis, the inner membrane flips to become outer membrane, thus exposing phosphatidyl serine (PS) which is sent to phagocytes to clear apoptotic cells.…”

Effect of Cheddar cheese peptide extracts on growth inhibition, cell cycle arrest and apoptosis induction in human lung cancer (H‐1299) cell line

“…It was considered that highly ripened cow milk cheeses were capable of demonstrating antiproliferative activities and induction of apoptotic DNA damage on human leukaemia (HL‐60) cells compared to low‐ripened cheeses (Yasuda et al . ). Similar findings have also been reported for 11 goat milk cheese on HL‐60 cells (Yasuda et al .…”

Anti‐inflammatory and anticancer activities of water‐soluble peptide extracts of buffalo and cow milk Cheddar cheeses

“…Likewise, some other studies showed that highly ripened cow milk cheeses were capable of demonstrating antiproliferative activities and apoptosis induction in HL‐60 cells in contrast to low‐ripened cheeses (Yasuda et al . ).…”

“…It is believed that these biologically active molecules with anticancer potential can be produced by the action of different types of microorganisms (bacterial proteolytic enzymes) during cheese manufacture. Likewise, some other studies showed that highly ripened cow milk cheeses were capable of demonstrating antiproliferative activities and apoptosis induction in HL-60 cells in contrast to low-ripened cheeses (Yasuda et al 2010).…”