Effect of Cheddar cheese peptide extracts on growth inhibition, cell cycle arrest and apoptosis induction in human lung cancer (H‐1299) cell line

Abstract

The objective of the study was to evaluate the anticancer activity of water-soluble peptide (WSP) extracts of buffalo and cow milk Cheddar cheeses during their ripening. The WSP extracts were assessed for their effects on cell viability, cell cycle arrest and apoptosis induction using a lung cancer (H-1299) cell line. Cheese extracts taken after 120 and 150 ripening days showed marked growth inhibition of the cancer cells, in a dose-dependent manner. During cell cycle analysis, a significant increase in cells population at G 2 /M phase was observed in the lung cancer cell line at a rate of 400 lg/mL and 500 lg/mL. In addition, the extracts also induced extensive apoptosis in the lung cancer cell line.

Evaluation of anti‐proliferative activity of Cheddar cheeses using colon adenocarcinoma (HCT‐116) cell line

“…The HCT‐116 (Human colon carcinoma) cell line (ATCC, Manassas, VA, USA) was maintained in Roswell Park Memorial Institute, RPMI‐1640 (Mediatech, Herndon, VA, USA) according to the detailed method given in our previous publication (Rafiq et al ).…”

“…MTT (3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide) assay was conducted to measure the effect of water‐soluble extract on viability of cancer cells (Rafiq et al ).…”

“…Human carcinoma cells (45 000 cells/mL) were seeded in 6‐well plates for 24 h incubation followed by treatment with serial concentrations of water‐soluble extract (400 and 500 μg/mL) and incubated again for 24 h (Rafiq et al ).…”

“…Annexin V‐fluorescein isothiocyanate (FITC)/PI apoptotic detection kit was used to quantify the cells. Detailed method is given in our previous publication (Rafiq et al ).…”

“…Protein hydrolysates and peptides derived from various foods have shown anti‐proliferative activities (Lahart et al ; Harnedy and FitzGerald ; Rafiq et al ). Especially, proteins from milk are good source of bioactive peptides which impart several biological activities including anti‐hypertension, anti‐oxidant, anti‐microbial, immune‐modulating, cholesterol lowering and anti‐cancer (Tagliazucchi et al ; Rafiq et al ; Kim et al ).…”