Evaluation of oxidative stress status and antioxidant capacity in patients with painful bladder syndrome/interstitial cystitis: preliminary results of a randomised study

Ener, Kemal; Keske, Murat; Aldemir, Mustafa; Ozcan, Muhammet Fuat; Okulu, Emrah; Özayar, Asım; Ergin, Merve; Doluoğlu, Ömer Gökhan; Cakmak, S.; Erel, Özcan

doi:10.1007/s11255-015-1021-1

Cited by 22 publications

(27 citation statements)

References 37 publications

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“…In IC, the mechanism of oxidative damage comes into prominence [26]. In addition, in our study, we found that the antioxidant biomarkers HO-1 and NQO-1 by Western blot and IF were notably increased in the tissue of the urinary bladder in IC animals compared to those in the control group.…”

Section: Discussionsupporting

confidence: 63%

Therapeutic effect of urine-derived stem cells for protamine/lipopolysaccharide-induced interstitial cystitis in a rat model

Luo

Dong

et al. 2017

Stem Cell Res Ther

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BackgroundInterstitial cystitis (IC) is a chronic inflammation disorder mainly within the submucosal and muscular layers of the bladder. As the cause of IC remains unknown, no effective treatments are currently available. Administration of stem cell provides a potential for treatment of IC.MethodsThis study was conducted using urine-derived stem cells (USCs) for protamine/lipopolysaccharide (PS/LPS)-induced interstitial cystitis in a rodent model. In total, 60 female Sprague–Dawley rats were randomized into three experimental groups (n = 5/group): sham controls; IC model alone; and IC animals intravenously treated with USCs (1.2 × 106 suspended in 0.2 ml phosphate-buffered saline (PBS).ResultsOur data showed that the bladder micturition function was significantly improved in IC animals intravenously treated with USCs compared to those in the IC model alone group. The amount of antioxidants and antiapoptotic protein biomarkers heme oxygenase (HO)-1, NAD(P)H quinine oxidoreductase (NQO)-1, and Bcl-2 within the bladder tissues were significantly higher in IC animals intravenously treated with USCs and lower in the sham controls group as assessed by Western blot and immunofluorescent staining. In addition, the expression of autophagy-related protein LC3A was significantly higher in the IC model alone group than that in IC animals intravenously treated with USCs. Inflammatory biomarkers and apoptotic biomarkers (interleukin (IL)-6, tumor necrosis factor (TNF)α, nuclear factor (NF)-κB, caspase 3, and Bax) and the downstream inflammatory and oxidative stress biomarkers (endoplasmic reticulum stress and autophagy-related protein (GRP78, LC3, Beclin1)) in the bladder tissue revealed statistically different results between groups.ConclusionsUSCs restored the bladder function and histological construction via suppressing oxidative stress, inflammatory reaction, and apoptotic processes in a PS/LPS-induced IC rodent model, which provides potential for treatment of patients with IC.

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Section: Discussionsupporting

confidence: 63%

Therapeutic effect of urine-derived stem cells for protamine/lipopolysaccharide-induced interstitial cystitis in a rat model

Luo

Dong

et al. 2017

Stem Cell Res Ther

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“…However, the investigation of serum levels of different oxidative stress markers is not new. Recently, Ener et al evaluated the oxidative stress status and antioxidant capacity in patients with painful bladder syndrome, and used serum total antioxidant capacity, total oxidant status, binding capacity of exogenous cobalt to human albumin, serum advanced oxidation protein products, paraoxonase, arylesterase, IgE, and C-reactive protein as oxidative markers (17). Some investigators researched the levels of oxidative markers either in urine or semen (18, 19).…”

Section: Discussionmentioning

confidence: 99%

The change in serum Thiol/Disulphide homeostasis after transrectal ultrasound guided prostate biopsy

et al. 2017

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Objectives The aim of this prospective clinical study was to investigate variations in a novel oxidative stress marker (thiol/disulphide homeostasis) in men who underwent transrectal ultrasound guided prostate biopsy (TRUSB).Materials and Methods A total of 22 men undergoing TRUSB of the prostate were enrolled in the study. Patients with abnormal digital rectal examination and/or total prostate specific antigen (PSA) over 4ng/mL underwent TRUSB with 12 cores. Serum samples were obtained before and just after the procedure to evaluate the possible changes in thiol/disulphide homeostasis. Mean age, total PSA and free PSA, prostate volume and histopathological data were also recorded.Results Mean age of the study population was 65.05±8.89 years. Significant decreases in native and total thiol levels were documented after the biopsy procedure. However, serum disulphide levels and disulphide/native thiol, disulphide/total thiol and native/total thiol ratios did not significantly change after TRUSB. No correlation was observed between oxidative parameters and total PSA and free PSA levels, prostate volume and histopathology of the prostate. However, mean patient age was significantly correlated with mean native and total thiol levels.Conclusion Significant decreases in serum native and total thiol levels related to the prostate biopsy procedure suggest that TRUSB causes acute oxidative stress in the human body. Since our trial is the first in the current literature to investigate these oxidative stress markers in urology practice, additional studies are warranted.

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“…Oxidative stress can lead to a variety of disorders, for example, metabolic syndrome [19], chronic inflammatory disease [20], or can be carcinogenic [21] respectively. Iron, as a transitional metal, is directly involved in the formation of ROS by donating and accepting electrons through the Fenton and Haber-Weiss reactions [22 Ener et al [23] showed that the dysregulation of ROS production plays an important role in IC. Decrease of oxidative stress by antioxidants significantly reduced inflammation in a rat model of IC [24].…”

Section: Physiology Of Iron In Inflammation and Ros Productionmentioning

confidence: 99%

Iron Chelation as Novel Treatment for Interstitial Cystitis

et al. 2019

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Interstitial cystitis (IC) is a highly prevalent debilitating disease, with its cardinal symptoms being severe pain, urinary urgency and frequency. The associated pain may eventually lead as a last resort to removal of the bladder. Though the initial trigger for IC remains largely unknown, we propose novel iron chelators as a possible new treatment for this disease. Iron is a mandatory component for the generation of reactive oxygen species (ROS). A substantial decrease in ROS production and thus inflammation can be achieved by effectively sequestering host iron, which we believe may improve outcome and quality of life in IC patients. Novel iron chelators could be used via the intravesical route to reduce or attenuate inflammation by effectively sequestering host iron, thus preventing the production of ROS via the Fenton and Haber-Weiss reactions.

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Evaluation of oxidative stress status and antioxidant capacity in patients with painful bladder syndrome/interstitial cystitis: preliminary results of a randomised study

Abstract: In the etiology of BPS/IC, mechanism of oxidative damage comes into prominence. In the diagnosis of BPS/IC, IgE, CRP, and TAC are not specific markers when used separately; however, a higher specifity and sensitivity could be reached when used jointly in the suspected patients.

Cited by 22 publications

References 37 publications

Therapeutic effect of urine-derived stem cells for protamine/lipopolysaccharide-induced interstitial cystitis in a rat model

Therapeutic effect of urine-derived stem cells for protamine/lipopolysaccharide-induced interstitial cystitis in a rat model

The change in serum Thiol/Disulphide homeostasis after transrectal ultrasound guided prostate biopsy

Iron Chelation as Novel Treatment for Interstitial Cystitis

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