Evaluation of Oxidative Status in Patients With Chronic Periodontitis and Additional Tobacco Abuse: A Cross-Sectional Study

Abstract

The aim of the survey was to evaluate the 8-hydroxy-2'-deoxyguanosine (8-OHdG) and melatonin (MLT) levels in saliva as well as myeloperoxidase (MPO) levels in gingival clevicular fluid (GCF) in smoker and nonsmoker chronic periodontitis (CP) patients and periodontally healthy individuals. Methods: 4 groups of 15 applicants were formed in accordance with the research protocole as follows; periodontally healthy nonsmokers (CP-S-), periodontally healthy smokers (CP-S+), nonsmoker periodontitis patients (CP+S-) and smoker periodontitis patients (CP+S+). Clinical periodontal status were measured before taking the saliva and GCF samples. 8-OHdG, MLT and MPO levels were measured biochemically. Results: The lowest level of 8-OHdG and MPO were recorded in (CP-S-) group (p<0.05). MLT levels in saliva were statistically increased in periodontally healthy (CP-) groups than CP groups (p<0.05). Conclusions: CP causes significant changes in parameters connected with oxidative stress. For periodontally healthy applicants, smoking leads to statistically significant changes on the parameters in general; while the effect of smoking to the parameters was found insignificant in the CP groups. Findings of this study reveal the role of smoking on the development of periodontitis, due to negatively affecting the parameters linked to oxidative stress, that are crucial in the patogenesis of periodontal diseases.