Evaluation of Oraquick® HIV-1/2 as oral rapid test

Nkenfou, Céline Nguefeu; Kembou, Japhette Esther; Temgoua, Edith; Djikeng, Appolinaire; Mouafo, Linda Mekue; Ndzie, Elvis Ndukong; Donkam, Irenee; Colizzi, V.; SanouSobzé, Martin

doi:10.4314/ajid.v7i2.2

Cited by 18 publications

(13 citation statements)

References 10 publications

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“…Screening for HIV sero-status was performed using OraQuick HIV (Ora Sure Technology, USA) test kits, as described by the manufacturer and as previously reported [7].…”

Section: Methodsmentioning

confidence: 99%

Antibiotic Resistance of Enteric Bacteria in HIV-Infected Patients at the Banka Ad-Lucem Hospital, West Region of Cameroon

Ngalani

Mbaveng

Marbou

et al. 2019

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Human immunodeficiency virus (HIV) infection is a serious problem throughout the world and especially in developing countries. This study was conducted to define the bacterial aetiologies of enteric disorders and their association with CD4+ T-lymphocyte cell count and serum hs-CRP in HIV-seropositive patients coming for consultation at the AD-Lucem Banka Hospital. Stool samples from one-hundred HIV-positive patients with enteric disorders and forty HIV negative patients with enteric disorders were examined for the presence of bacteria by different diagnostic techniques. CD4+ T-lymphocyte count and serum hs-CRP of HIV-positive and HIV-negative patients were examined, respectively, by flux cytometry and the ELISA solid-phase direct sandwich method. Among all the participants, 39 (26.35%) were males and 109 (73.65%) were females. HIV-seropositive mean age (43.79 years) was significantly higher compared to HIV-seronegative (27.13 years) patients (p<0.000). The average values of CD4+ T-cell count (p<0.0001), lymphocytes (p=0.0258), monocytes (p=0.0317), and total WBC count (p=0.0277) were significantly higher in HIV− patients compared to HIV+ patients. Salmonella sp., Escherichia coli, and Klebsiella pneumoniae were more isolated in HIV+ patients 5 (83.33), 18 (75.00), and 37 (71.15) compared to HIV− patients 1 (16.67), 6 (25.00), and 15 (28.85), respectively. Majority of isolates were susceptible to IPM, NOR, and CIP. Klebsiella pneumoniae, the most prevalent isolate, showed resistance to AMC (45.95) in HIV+ patients, whereas in HIV− patients, Enterobacter aerogenes and Shigella sp. showed resistance to AMC (80.00% and 85.71%, respectively) and to CFM (80.00% and 57.14%, respectively). Enterobacter aerogenes (40.00%) and Shigella sp. (14.29) isolates showed multidrug resistance in HIV− patients, whereas Escherichia coli (5.56%) and Klebsiella pneumoniae (2.70%) showed multidrug resistance in HIV+ patients. Understanding the burden of bacteria disease in HIV patients as shown in the present study is important for planning effective control programs for the overall reduction of bacteria diseases in HIV-infected patients.

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“…Screening for HIV sero-status was performed using OraQuick HIV (Ora Sure Technology, USA) test kits, as described by the manufacturer and as previously reported [7].…”

Section: Methodsmentioning

confidence: 99%

Antibiotic Resistance of Enteric Bacteria in HIV-Infected Patients at the Banka Ad-Lucem Hospital, West Region of Cameroon

Ngalani

Mbaveng

Marbou

et al. 2019

Canadian Journal of Infectious Diseases and Medical Microbiolog

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“…Participants in the HIV self‐testing intervention arms received either an HIV self‐test kit or a coupon for an HIV self‐test kit from their peer educator shortly after enrolment and again three months later. We used the OraQuick Rapid HIV‐1/2 Antibody Tests (OraSure Technologies, Bethlehem, PA) in this study, which have 93% sensitivity and 99% specificity compared to standard‐of‐care blood‐based HIV testing . All participants were trained by an FSW peer educator on how to use and interpret the HIV self‐test results.…”

Section: Methodsmentioning

confidence: 99%

Is knowledge of HIV status associated with sexual behaviours? A fixed effects analysis of a female sex worker cohort in urban Uganda

Ortblad

Musoke²,

Ngabirano

et al. 2019

J Intern AIDS Soc

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Introduction Female sex workers (FSWs) have strong economic incentives for sexual risk‐taking behaviour. We test whether knowledge of HIV status affects such behaviours among FSWs. Methods We used longitudinal data from a FSW cohort in urban Uganda, which was formed as part of an HIV self‐testing trial with four months of follow‐up. Participants reported perceived knowledge of HIV status, number of clients per average working night, and consistent condom use with clients at baseline, one month, and four months. We measured the association between knowledge of HIV status and FSWs’ sexual behaviours using linear panel regressions with individual fixed effects, controlling for study round and calendar time. Results Most of the 960 participants tested for HIV during the observation period (95%) and experienced a change in knowledge of HIV status (71%). Knowledge of HIV status did not affect participants’ number of clients but did affect their consistent condom use. After controlling for individual fixed effects, study round and calendar month, knowledge of HIV‐negative status was associated with a significant increase in consistent condom use by 9.5 percentage points (95% CI 5.2 to 13.5, p < 0.001), while knowledge of HIV‐positive status was not associated with a significant change in consistent condom use (2.5 percentage points, 95% CI −8.0 to 3.1, p = 0.38). Conclusions In urban Uganda, FSWs engaged in safer sex with clients when they perceived that they themselves were not living with HIV. Even in communities with very high HIV prevalence, the majority of the population will test HIV‐negative. Our results thus imply that expansion of HIV testing programmes may serve as a behavioural HIV prevention measure among FSWs.

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“…More false–positive tests were observed in this study than true–positive tests, yielding a positive predictive value (PPV) of ∼47%. This was much lower than the performance characteristics reported by the manufacturer and others including in low‐risk populations 10,40,41 . However, multiple “real‐world” settings have experienced similar issues with false–positives while using the oral version of the OraQuick test 42,43 .…”

Section: Discussionmentioning

confidence: 73%

Testing for HIV infection in the emergency departments of 2 hospitals in the Southeastern United States

et al. 2020

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Background In 2006, the Centers for Disease Control and Prevention (CDC) recommended non‐targeted, opt‐out HIV screening in all healthcare settings, including emergency departments (EDs). Multiple HIV testing programs have been implemented in EDs across the United States with varying designs and testing platforms. We report findings from a free, non‐targeted, rapid HIV testing program in 2 EDs in the Southeastern United States. Methods From 2008 to 2012, adults ≥18 years of age were offered free rapid HIV testing using an oral swab test (OraQuick ADVANCE Rapid HIV‐1/2 antibody test) in the EDs of a large academic medical center and an affiliated community hospital in Durham, North Carolina. Results In total, 5443 ED patients were offered HIV testing. The overall acceptance rate was 66.9% (3639/5443). Younger persons were significantly more likely to accept testing (78.2% for 18–29 years old vs 67.1% for ≥30 years old; P < 0.001) as were Black participants (72.6% Black vs 66.5% White; P < 0.001). Acceptance rates improved significantly after opt‐out oral consent replaced written consent (71.3% vs 63.1%; P < 0.001). Seven new HIV diagnoses were confirmed during the testing program, resulting in a seropositivity rate of 0.19% (7/3639). There were 8 false–positive rapid oral HIV tests (positive predictive value = 46.7%). Conclusions Although the number of new HIV diagnoses was low, implementation of this rapid, non‐targeted ED screening program was feasible with high acceptance rates, particularly after introducing the opt‐out oral consent approach.

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Evaluation of Oraquick® HIV-1/2 as oral rapid test

Cited by 18 publications

References 10 publications

Antibiotic Resistance of Enteric Bacteria in HIV-Infected Patients at the Banka Ad-Lucem Hospital, West Region of Cameroon

Antibiotic Resistance of Enteric Bacteria in HIV-Infected Patients at the Banka Ad-Lucem Hospital, West Region of Cameroon

Is knowledge of HIV status associated with sexual behaviours? A fixed effects analysis of a female sex worker cohort in urban Uganda

Testing for HIV infection in the emergency departments of 2 hospitals in the Southeastern United States

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