Evaluation of Oral Mucosa Irritation produced by Ozone Gel

Fukui, Tatsuya; Masuno, Kazuya; Makita, Yasuharu; Fujiwara, Shin-ichi; Shiota, Gotaro; Imamura, Yasuhiro; Shiba, Akihiko; Wang, Pao-Li

doi:10.2485/jhtb.24.104

Cited by 3 publications

(3 citation statements)

References 7 publications

(9 reference statements)

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“…Recent studies have shown that OW is less irritating to the skin than traditional disinfectants, raising interest in OW as an alternative handwashing treatment (Breidablik et al, 2019 ; Kashiwazaki et al, 2020 ). Likewise, OG is also less irritating to the skin and has a longer ozone half-life than OW (Fukui et al, 2015 ; Wang et al, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

“…As its name implies, ozonated glycerol (OG) is a glycerol gel containing ozone (Niinomi et al, 2004 ). More specifically, OG has been reported to have a long half-life (Niinomi et al, 2004 ), be safe for the skin, eyes, and oral mucosa (Fukui et al, 2015 ; Wang et al, 2011 ), as well as exert antibacterial (Niinomi et al, 2004 ; Fukui et al, 2014 ) and hemostatic (Sakai et al, 2014 ) effects.…”

Section: Introductionmentioning

confidence: 99%

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Inactivation of SARS-CoV-2 by Ozonated Glycerol

et al. 2021

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We evaluated the SARS-CoV-2-inactivation activity of ozonated glycerol (OG). When a viral solution with 1% fetal bovine serum (FBS) was mixed with test solutions at a ratio of 1:19 and incubated for 20 s, OG with ozone concentrations of over 1000 ppm inactivated ≥ 94.38% of the virus. Extension of the reaction time to 1 h led to the inactivation of ≥ 99.82% of the virus (the viral titer was below the detection limit). Extension to 24 h resulted in concentrations over 200 ppm OG inactivating ≥ 99.87% of the virus (the viral titers were below the detection limit). Next, viral solutions with 1, 20, and 40% FBS were mixed with test solutions at a ratio of 1:19 and incubated for 5 min. Whereas the virucidal activity of 500 ppm OG was very limited in the presence of 1% FBS (79.47% inactivation), it increased in the presence of 20 and 40% FBS (95.13 and 97.95% inactivation, respectively; the viral titers were not below the detection limit). Meanwhile, over 1000 ppm OG inactivated ≥ 99.44% of the virus regardless of the FBS concentration (the viral titers were below the detection limit). Extension of the reaction time to 1 h led to 500 ppm OG inactivating ≥ 99.91 and ≥ 99.95% of the virus with 20 and 40% FBS, respectively (the viral titers were below the detection limit). These results suggested that OG might be useful as a virucidal agent against SARS-CoV-2.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inactivation of SARS-CoV-2 by Ozonated Glycerol

et al. 2021

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“…1). The evaluation of oral mucosal irritation was performed using the method described in the previous reports (CTFA Safety Testing Guidelines, 1981;Fukui et al, 2015). Oral mucosal irritation was graded according to the Table 1, and irritancy scores were calculated by averaging the combined daily grades for the tongue dorsum, and labial mucosa and incisor gingiva of the mandibular oral vestibule.…”

Section: Macroscopic Observations Of Oral Mucosamentioning

confidence: 99%

A simple method for oral mucosal irritation test by intraoral instillation in rats

et al. 2016

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-Evaluation of oral mucosal irritation is required by regulatory agencies when the intended clinical route of the drug candidate is intraoral administration. In this study, we investigated whether it was possible to evaluate oral mucosal irritation in rats by an intraoral instillation which was thought to mimic the clinical route of gargle products. Although no oral mucosal irritation was observed in the animals instilled with 0.5% and 4% sodium dodecyl sulfate (SDS, an anionic detergent) solutions for 10 days, instillation of 15% SDS solution for 4 days induced oral mucosal irritation macro-and microscopically, and this was evaluated as moderate irritant. It was suggested that the oral mucosal irritation test by intraoral instillation in rats could be a simple and useful method mimicking the clinical route of gargle products.

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Novel bicephalous heterolipid based self-microemulsifying drug delivery system for solubility and bioavailability enhancement of efavirenz

Chaudhari

Akamanchi

2019

International Journal of Pharmaceutics

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Evaluation of Oral Mucosa Irritation produced by Ozone Gel

Cited by 3 publications

References 7 publications

Inactivation of SARS-CoV-2 by Ozonated Glycerol

Inactivation of SARS-CoV-2 by Ozonated Glycerol

A simple method for oral mucosal irritation test by intraoral instillation in rats

Novel bicephalous heterolipid based self-microemulsifying drug delivery system for solubility and bioavailability enhancement of efavirenz

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