Evaluation of nutritional and biochemical parameters in spontaneously hypertensive rats following antihypertensive treatment

Abstract

Introduction. One side effect of antihypertensive drugs is their impact on nutritional status and metabolism. The purpose of this study was to assess the nutritional and biochemical parameters in spontaneously hypertensive rats following treatment with antihypertensive drugs. Material and methods. The experiment was performed on 50 male spontaneously hypertensive rats (SHR), which were assigned to fi ve groups: control (C), with perindopril (PR), with metoprolol (MT), with indapamide (ID), and with amlodipine (AM). All rats were provided ad libitum standard diet (with or without drugs) and distilled water. After 45 days, the animals were weighed and killed. Liver, kidney, heart, spleen, pancreas, and blood samples were collected. Concentrations of glucose, cholesterol, triglycerides, and albumin were assayed in serum. Morphology parameters, such as white blood cell, red blood cell, hematocrit, and lymphocyte counts were measured in the blood. Blood pressure was measured using a tail-cuff plethysmograph. Results. The results obtained indicate that the hypotensive drugs under investigation had no effect on the selected nutritional parameters. Perindopril signifi cantly decreased the relative mass of the heart and amlodipine markedly decreased the relative mass of the pancreas. A markedly higher concentration of glucose in the group with indapamid, and a signifi cantly lower concentration of triglycerides in the group with metoprolol, were observed. Indapamide and amlodipine markedly increased the value of red blood cells and hematocrit in the blood of SHR. Conclusions. Long-term therapy with antihypertension drugs may infl uence tissue mass and biochemical and morphological status in the body.

Pharmacological Treatment may Impair Mineral Status in Blood

“…Moreover, the decrease in zinc is accompanied by an increase in glucose serum levels [2]. In our experimental study, we observed that treatment with indapamide and amlodipine was associated with increased red blood cell counts and hematocrit values in the serum of spontaneously hypertensive rats [3]. In patients, high hematocrit could reflect the increase in red blood cell mass resulting from the reduction in plasma volume due to treatment with thiazide-type diuretics.…”

Hemorheological effects of amlodipine in spontaneously hypertensive rats

“…Similar results were also obtained in another study performed on hypertensive animals. [ 17 ] In the literature, there is no information about changes in RBC quantity or volume in SHRs under the influence of amlodipine. On the other hand, it is well known that amlodipine has a slight diuretic effect, which may cause a reduction in plasma volume.…”

Iron bioavailability from cereal products enriched with Pleurotus ostreatus mushrooms in rats with induced anaemia

“…At the same time, it needs to be stressed that already enrichment of the product with a 10% addition of dried oyster mushroom caused a significant restoration of systemic iron level in those animals. Suliburska [23,24] expressed an opinion that products enriched with Fe are better sources of potentially available Fe than products not enriched with this nutrient, but the sorption of minerals depended on the dietary fibre source, adsorbent type, processing method, pH and temperature of the medium, as well as on the metal. Regula and Gramza-Michalowska [25] observed in in vitro experiments, maximum sorption values at pH = 8.7 for Fe in bread with 10% and 20% dried mushrooms added (15% and 17%, respectively).…”