Evaluation of nutrient uptake and physical parameters on cell biomass growth and production of spilanthol in suspension cultures of Spilanthes acmella Murr.

Abstract

Spilanthes acmella Murr. has a plethora of highly valuable biologically active compounds and has been listed as one of the important medicinal plants of the world. However, no perceptible biotechnological advances have been made for this genus to exploit or enhance its utility. To nullify the effect of seasonal variations, the present report is the first attempt to establish in vitro cell suspension cultures and to evaluate the production of spilanthol from them, a key component of the plant responsible for most of its pharmaceutical activities. The study examined the biomass growth in relation to the consumption of major nutrients and sucrose, agitation speed and dynamic change in pH. Results indicated that the consumption of phosphate resulted in the onset of decline phase in cultures. Spilanthol production was observed to be growth associated and maximum production occurred on the 15th day. Among the carbon sources, the highest production of spilanthol as 91.4 µg g(-1) DW was recorded in the medium supplemented with sucrose, followed by glucose which produced 56.8 µg g(-1) DW of spilanthol. Spilanthol could not be detected in fructose containing medium. Maximum viable cultures were obtained at a rotation speed of cells at 120 rpm. This study signifies the utility of Spilanthes suspension cultures for biosynthesis and constant production of spilanthol, throughout the year. The results of present study are useful for further scale-up process.

Enhanced Biosynthesis of Withanolides by Elicitation and Precursor Feeding in Cell Suspension Culture of Withania somnifera (L.) Dunal in Shake-Flask Culture and Bioreactor

“…Plant cell/organ cultures have been perceived as promising choice to traditional plant extraction for obtaining valuable chemicals throughout the year. Particularly, cell suspension culture offers a condensed biosynthetic cycle to study the growth and production kinetics within a short cultivation time (about 2–4 weeks) with an added advantage of tunability that can help to implement optimal conditions for the production of a number of high value medicinal compounds in good quantities [2], [3].…”

Jambu drying with cold air circulation

“…They are the main constituents responsible for the pharmacological activity (RADUNER et al, 2006). Due to the spilanthol amide, the jambu is widely used in pharmaceutical products, foods and health and body care products (PANDEY &AGRAWAL, 2009;SINGH & CHATURVEDI, 2012).…”

Natural and Synthetic Alkamides

“…Affinin is also the main component in at least five other species of Asteraceae family: Wedelia parviceps [175], Acmella ciliata, A. oleracea, A. oppositifolia [176] and Spilanthes acmella [177,178]. They all have been used as a spice and also in folk medicine since ancient times to cure severe toothache, infections of throat and gums, stomatitis, paralysis of tongue, antiseptic, analgesic, and immunemodulators.…”

“…These Studies in Natural Products Chemistry findings are suggestive that affinin can be a useful inhibitor of inflammatory mediators and is potentially applicable for COX-2 selective nonsteroidal anti-inflammatory drugs [180]. Currently, biosynthesis and production of spilanthol from in vitro cells and tissue cultures of S. acmella are promising tools useful for the purpose of scale-up processes [177,181,182]. Another specie used as anesthetic is Acmella decumbens and its content of alkamides has been established [183].…”