Evaluation of novel magnetic targeting microspheres loading adriamycin based on carboxymethyl chitosan

Synthesis, Characterization, and Evaluation of Nanoparticles Loading Adriamycin Based on 2-Hydroxypropyltrimethyl Ammonium Chloride Chitosan Grafting Folic Acid

“…a broadening peak at about 3421 cm −1 , the characteristic peak appears at 1023 cm −1 (the C=O stretching vibration of ADM), and a new peak at 613 cm −1 (the C-H stretching vibration of ADM), indicating the ADM was loaded on the nanoparticles [3]. Therefore, the data confirmed the successful preparation of chitosan derivatives and chitosan nanoparticles.…”

“…In case of HACFNP, in addition to the singles located at 1487 cm −1 and 1416 cm − 1 of the trimethylammonium group, a new peak belonging to the C=O stretching vibration of the carboxyl in folate molecule at 1692 cm −1 also appears. Compared to the blank nanoparticles (HACFNP), nanoparticles (HACAFNP) loading ADM based on HACF demonstrated a broadening peak at about 3421 cm −1 , the characteristic peak appears at 1023 cm − 1 (the C=O stretching vibration of ADM), and a new peak at 613 cm −1 (the C-H stretching vibration of ADM), indicating the ADM was loaded on the nanoparticles [ 3 ]. Therefore, the data confirmed the successful preparation of chitosan derivatives and chitosan nanoparticles.…”

“…The cytotoxicities of chitosan, chitosan derivatives, and chitosan nanoparticles on four kinds of cells (three kinds of cancer cells, including MCF-7, BGC-823, and HEPG-2, and normal cells HTR-8) were conducted via CCK-8 assay using the previous method in vitro [ 3 ]. Taking MCF-7 as an example, MCF-7 cells were seeded on 96-well flat-bottom culture plates with the concentration of 1.0 × 10 5 cells in 100 µL of RPMI medium, including the mixture of penicillin and streptomycin (1%) and fetal calf serum (10%), and incubated at 37 °C under a CO 2 atmosphere (5%).…”

“…positively charged chitosan nanoparticles are absorbed by the cells more easily because of the negative charge on the cell membrane [3]. Meanwhile, from Figure 3b, with the passage of time, the change of particle size of different nanoparticles was relatively small, which indicates that the nanoparticles had good stability and could serve as practical drug delivery system.…”

“…Despite the rapid development of technology and medicine in the 21st century, cancer is still one of the major diseases that can threaten the safety and health of human life all over the world [ 1 , 2 ]. There are three kinds of traditional cancer treatments: surgical operation, chemotherapy, and radiotherapy [ 3 ]. Depending on the type of cancer, the stage of development, and the general condition of patient, the treatment strategy may vary.…”