Evaluation of nonlinear optical differences between breast cancer cell lines SK-BR-3 and MCF-7; an in vitro study

Ghader, Alireza; Ardakani, Ali Abbasian; Ghaznavi, Habib; Shakeri‐Zadeh, Ali; Minaei, Soraya Emamgholizadeh; Mohajer, Salman; Ara, M.H. Majles

doi:10.1016/j.pdpdt.2018.06.015

Cited by 19 publications

(9 citation statements)

References 21 publications

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“…In the present study, we also observed low miR-92b and high XIST expression concurrently in breast cancer tissues and cell lines. These findings were in agreement with several studies on XIST and miR-92b expression in tumors [13][14][15][36][37][38].…”

Section: Discussionsupporting

confidence: 93%

“…We report that lncRNA XIST (X-inactive-specific transcript), which is involved in genomic imprinting and the transcriptional silencing of genes on the X chromosome and is highly expressed in tumor tissues [11][12][13][14][15], is an epigenetic regulator targeted by miR-7 that directly bound and inhibited XIST expression in breast cancer by using the RNA in vivo precipitation (RIP) method, which underlies the attenuation of properties of BCSCs and the decrease of the BCSC subset. MiR-7 also modulated the miR-92b/Slug/ESA (epithelium-specific antigen) axis and reduced CD44 and Slug expression, resulting in a decrease in cancer cell stemness and the BCSC subset in breast cancer.…”

Section: Introductionmentioning

confidence: 99%

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MiR-7 reduces the BCSC subset by inhibiting XIST to modulate the miR-92b/Slug/ESA axis and inhibit tumor growth

Pan

You

et al. 2020

Breast Cancer Res

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Background: Breast cancer stem cells (BCSCs) are typically seed cells of breast tumor that initiate and maintain tumor growth. MiR-7, as a cancer inhibitor, decreases the BCSC subset and inhibits tumor progression through mechanisms that remain unknown. Methods: We examined miR-7 expression in breast cancer and developed a BCSC-driven xenograft mouse model, to evaluate the effects of miR-7 overexpression on the decrease of the BCSC subset in vitro and in vivo. In addition, we determined how miR-7 decreased the BCSC subset by using the ALDEFLUOR, lentivirus infection, dual-luciferase reporter, and chromatin immunoprecipitation-PCR assays. Results: MiR-7 was expressed at low levels in breast cancer tissues compared with normal tissues, and overexpression of miR-7 directly inhibited lncRNA XIST, which mediates the transcriptional silencing of genes on the X chromosome, and reduced epithelium-specific antigen (ESA) expression by increasing miR-92b and inhibiting slug. Moreover, miR-7 suppressed CD44 and ESA by directly inhibiting the NF-κB subunit RELA and slug in breast cancer cell lines and in BCSC-driven xenografts, which confirmed the antitumor activity in mice injected with miR-7 agomir or stably infected with lenti-miR-7. Conclusions: The findings from this study uncover the molecular mechanisms by which miR-7 inhibits XIST, modulates the miR-92b/Slug/ESA axis, and decreases the RELA and CD44 expression, resulting in a reduced BCSC subset and breast cancer growth inhibition. These findings suggest a potentially targeted treatment approach to breast cancer.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

MiR-7 reduces the BCSC subset by inhibiting XIST to modulate the miR-92b/Slug/ESA axis and inhibit tumor growth

Pan

You

et al. 2020

Breast Cancer Res

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“…However, at 24 and 48 h, a positive refractive nonlinearity was witnessed with a structure of prefocal valley and postfocal peak, which is because of the effect of selffocusing. While this switching of the sign was previously observed in breast cancer cell lines, organic dyes, and other materials, 23,25,45 this feature has never been used as a marker toward identifying different conformations of a protein. We noted that this remarkable sign change in the case of α-syn first occurs at 24 h, and this time window also corresponds to where the late oligomers start forming.…”

Section: ■ Results and Discussionmentioning

confidence: 82%

“…From the past approximately three decades, the Z-scan technique is extensively recognized in the community of nonlinear optics as a standard experimental method to determine nonlinear parameters, owing to its simplicity and high sensitivity. − Principally, a sample is scanned across the focal plane of a focused Gaussian beam, and the transmittance through the sample is measured, at the far-field, as a function of its position ( z ). If the transmittance profile is measured by means of a finite circular aperture in front of the detector, it is termed a closed-aperture (CA) Z-scan technique.…”

Section: Introductionmentioning

confidence: 92%

A Novel Tool to Investigate the Early and Late Stages of α-Synuclein Aggregation

Ghosh

Sakshi

Swain

et al. 2020

ACS Chem. Neurosci.

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The accumulation of an inherently disordered protein α-synuclein (α-syn) aggregates in brain tissue play a pivotal role in the pathology and etiology of Parkinson’s disease. Aggregation of α-syn has been found to be complex and heterogeneous, occurring through multitudes of early- and late-stage intermediates. Because of the inherent complexity and large dynamic range (between a few microseconds to several days under in vitro measurement conditions), it is difficult for the conventional biophysical and biochemical techniques to sample the entire time window of α-syn aggregation. Here, for the first time, we introduced the Z-scan technique as a novel tool to investigate different conformations formed in the early and late stage of temperature and mechanical stress-induced α-syn aggregation, in which different species showed its characteristic nonlinear characteristics. A power-dependent study was also performed to observe the changes in the protein nonlinearity. The perceived nonlinearity was accredited to the thermal-lensing effect. A switch in the sign of the refractive nonlinearity was observed for the first time as a signature of the late oligomeric conformation, a prime suspect that triggers cell death associated with neurodegeneration. We validate Z-scan results using a combination of different techniques, like thioflavin-T fluorescence assay, fluorescence correlation spectroscopy, Fourier-transform infrared spectroscopy, and atomic force microscopy. We believe that this simple, inexpensive, and sensitive method can have potential future applications in detecting/monitoring conformations in other essential peptides/proteins related to different neurodegenerative and other human diseases.

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“…Compared with the MCF-7 cell line, the MDA-MB-231, SK-BR-3, and LD cell lines exhibited high expression of ESAM and low expression of miR-7. It is known that MCF-7 cells are duct cell carcinoma with estrogen receptor (ER + ), progesterone receptor (PR + ), and epidermal growth factor receptor 2 (HER2 À ), 16 while the MDA-MB-231 cells are ER À /PR À /HER2 À (triple-negative breast cancer cells), the SK-BR-3 cells are ER À /PR À /HER2 + , 17,18 and the LD cells are ER + /PR + /HER2 + . Owing to the high expression of ESAM, a molecule tightly related to tumor cell metastasis, the cells with high ESAM expression may pose a higher capability of invasion and metastasis than do the MCF-7 cells, which are relative low malignancy.…”

Section: Discussionmentioning

confidence: 99%

miR-7 Reduces Breast Cancer Stem Cell Metastasis via Inhibiting RELA to Decrease ESAM Expression

Pan

Wang

et al. 2020

Molecular Therapy - Oncolytics

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This study aimed to present evidence that miR-7 inhibited the metastasis of breast cancer stem cells (BCSCs) and elucidated the mechanisms that have remained unknown. The samples collected from miR-7 agomir-treated, BCSC-driven tumors were subjected to a protein array to analyze the protein expression profiles. A dual-luciferase reporter and chromatin immunoprecipitation-PCR were used to validate and evaluate the molecular expressions of interest in the collected breast cancer tissues and cell lines. miR-7 overexpression affecting metastasis of BCSCs was further evaluated in mice. The endothelial cell-selective adhesion molecule (ESAM) was highly expressed in breast cancer tissues and in BCSC-driven xenografts. Results of the dual-luciferase reporter and chromatin immunoprecipitation-PCR indicated that the miR-7 mimic reduced RELA expression by directly targeting the 3 0 UTR of RELA to inhibit ESAM expression in MDA-MB-231 cells. Moreover, the expression levels of RELA, CD44, and ESAM were significantly decreased in lentivirus (Lenti)-miR-7-BCSCdriven xenografts compared with the control xenografts, accompanied with an increase in E-cadherin and a decrease in vimentin expression, as well as reduction in tumor growth and metastasis to lungs. Our data demonstrated that miR-7 overexpression reduced the metastasis of BCSCs via inhibiting ESAM, suggesting that ESAM could be a potential target for breast cancer therapy.

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Evaluation of nonlinear optical differences between breast cancer cell lines SK-BR-3 and MCF-7; an in vitro study

Cited by 19 publications

References 21 publications

MiR-7 reduces the BCSC subset by inhibiting XIST to modulate the miR-92b/Slug/ESA axis and inhibit tumor growth

MiR-7 reduces the BCSC subset by inhibiting XIST to modulate the miR-92b/Slug/ESA axis and inhibit tumor growth

A Novel Tool to Investigate the Early and Late Stages of α-Synuclein Aggregation

miR-7 Reduces Breast Cancer Stem Cell Metastasis via Inhibiting RELA to Decrease ESAM Expression

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