Evaluation of non-thermal plasma-induced anticancer effects on human colon cancer cells

Choi, Jae-Sun; Kim, Jeongho; Hong, Young-Jun; Bae, Woom-Yee; Choi, Eun Ha; Jeong, Joo‐Won; Park, Hun-Kuk

doi:10.1364/boe.8.002649

Cited by 26 publications

(19 citation statements)

References 40 publications

(14 reference statements)

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“…Choi et al . [ 31 ] utilized PCA-DA to differentiate plasma-treated cells from control cells. Their classification results were accurate, sensitive, and specific (91%, 87%, and 99%, respectively).…”

Section: Discussionmentioning

confidence: 99%

Label-free Detection for a DNA Methylation Assay Using Raman Spectroscopy

Kim

Chang

et al. 2017

Chinese Medical Journal

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Background:DNA methylation has been suggested as a biomarker for early cancer detection and treatment. Varieties of technologies for detecting DNA methylation have been developed, but they are not sufficiently sensitive for use in diagnostic devices. The aim of this study was to determine the suitability of Raman spectroscopy for label-free detection of methylated DNA.Methods:The methylated promoter regions of cancer-related genes cadherin 1 (CDH1) and retinoic acid receptor beta (RARB) served as target DNA sequences. Based on bisulfite conversion, oligonucleotides of methylated or nonmethylated probes and targets were synthesized for the DNA methylation assay. Principal component analysis with linear discriminant analysis (PCA-DA) was used to discriminate the hybridization between probes and targets (methylated probe and methylated target or nonmethylated probe and nonmethylated target) of CDH1 and RARB from nonhybridization between the probe and targets (methylated probe and nonmethylated target or nonmethylated probe and methylated target).Results:This study revealed that the CDH1 and RARB oligo sets and their hybridization data could be classified using PCA-DA. The classification results for CDH1 methylated probe + CDH1 methylated target versus CDH1 methylated probe + CDH1 unmethylated target showed sensitivity, specificity, and error rates of 92%, 100%, and 8%, respectively. The classification results for the RARB methylated probe + RARB methylated target versus RARB methylated probe + RARB unmethylated target showed sensitivity, specificity, and error rates of 92%, 93%, and 11%, respectively.Conclusions:Label-free detection of DNA methylation could be achieved using Raman spectroscopy with discriminant analysis.

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Section: Discussionmentioning

confidence: 99%

Label-free Detection for a DNA Methylation Assay Using Raman Spectroscopy

Kim

Chang

et al. 2017

Chinese Medical Journal

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“…Therapeutic uses include the treatment of skin diseases [94], blood coagulation [95] as well as dental treatment [96] and applications in dermatology such as chronic wound healing [97]. Recently, the potential application of plasma technology as a novel anticancer therapy has been assessed [98]. Induction of cancer cell death by both direct and indirect exposure of plasma has been reported [99,100].…”

Section: Future Perspectives In Medicine and Dentistrymentioning

confidence: 99%

Disinfection and Sterilization Using Plasma Technology: Fundamentals and Future Perspectives for Biological Applications

Sakudo

Yagyu

Onodera

2019

IJMS

233

178

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Recent studies have shown that plasma can efficiently inactivate microbial pathogens such as bacteria, fungi, and viruses in addition to degrading toxins. Moreover, this technology is effective at inactivating pathogens on the surface of medical and dental devices, as well as agricultural products. The current practical applications of plasma technology range from sterilizing therapeutic medical devices to improving crop yields, as well as the area of food preservation. This review introduces recent advances and future perspectives in plasma technology, especially in applications related to disinfection and sterilization. We also introduce the latest studies, mainly focusing on the potential applications of plasma technology for the inactivation of microorganisms and the degradation of toxins.

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“…(2) Therefore, CAP has potential for biomedical applications such as wound healing, (3)(4)(5)(6) infection control, (7)(8)(9)(10) and cancer therapy. (11)(12)(13)(14) There are at least three types of CAP used for biological samples: 1) plasma jets, 2) corona discharge plasma sources, and 3) dielectric barrier discharge plasma sources. (4) Plasma jets are easy to handle and convenient to apply to in vivo samples, such as mice and humans, in addition to in vitro samples such as cell suspensions and membrane vesicles.…”

mentioning

confidence: 99%

Formation of reactive oxygen species by irradiation of cold atmospheric pressure plasma jet to water depends on the irradiation distance

Anzai

Aoki

Koshimizu

et al. 2019

J. Clin. Biochem. Nutr.

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Because application of cold atmospheric pressure plasma jet (CAPPJ) to biological samples have taken large attentions, it is important to examine the effects of various CAPPJ parameters on the generation of reactive species. Here, we investigated the generation of reactive species in water by CAPPJ irradiation by changing the following parameters: irradiation time, sample volume, and irradiation distance between the sample surface and plasma jet tip. We measured 1) change in the ESR signal intensity of 4 hydroxy 2,2,6,6 tetrametylpeperidine 1 oxyl (Tempol), 2) spin trapping with 5,5 dimethyl 1 pyrroline N oxide (DMPO), 3) Fricke dosimeter reaction, and 4) hydrogen peroxide (H 2 O 2) formation induced by CAPPJ irradiation. By the experiment of volume dependency, it is suggested that the reactive species detected in water are formed largely in the plasma gas phase. The reduction of ESR signal intensity of Tempol and the formation of DMPO OH were strongly dependent on irradiation distance, but the relation ship between H 2 O 2 generation and distance was weak. The forma tion of species that oxidize Fe 2+ to Fe 3+ was shown by the Fricke dosimeter reaction, and reactions at irradiation distances longer than 3 cm were mainly attributable to H 2 O 2. It may be possible to apply different reactive species to the samples by changing the CAPPJ irradiation distance.

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Evaluation of non-thermal plasma-induced anticancer effects on human colon cancer cells

Cited by 26 publications

References 40 publications

Label-free Detection for a DNA Methylation Assay Using Raman Spectroscopy

Label-free Detection for a DNA Methylation Assay Using Raman Spectroscopy

Disinfection and Sterilization Using Plasma Technology: Fundamentals and Future Perspectives for Biological Applications

Formation of reactive oxygen species by irradiation of cold atmospheric pressure plasma jet to water depends on the irradiation distance

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