Disinfection and Sterilization Using Plasma Technology: Fundamentals and Future Perspectives for Biological Applications

Sakudo, Akikazu; Yagyu, Yoshihito; Onodera, Takashi

doi:10.3390/ijms20205216

Cited by 235 publications

(216 citation statements)

References 101 publications

(149 reference statements)

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“…Thus, reactive chemical species such as OH and H radicals may act to eliminate bacteria by oxidizing biomolecules. Indeed, these findings are consistent with a previous study that showed that the OH radical is the most important factor for bacterial inactivation during plasma treatment [ 16 , 30 ]. Thus, oxidation and degradation of biomolecules may be caused by reactive chemical species such as reactive nitrogen species (RNS) and reactive oxygen species (ROS) generated by the plasma.…”

Section: Discussionsupporting

confidence: 93%

Antibiotic-Resistant and Non-Resistant Bacteria Display Similar Susceptibility to Dielectric Barrier Discharge Plasma

Sakudo

Misawa

2020

IJMS

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Here, we examined whether antibiotic-resistant and non-resistant bacteria show a differential susceptibility to plasma treatment. Escherichia coli DH5α were transformed with pPRO-EX-HT-CAT, which encodes an ampicillin resistance gene and chloramphenicol acetyltransferase (CAT) gene, and then treated with a dielectric barrier discharge (DBD) plasma torch. Plasma treatment reduced the viable cell count of E. coli after transformation/selection and further cultured in ampicillin-containing and ampicillin-free medium. However, there was no significant difference in viable cell count between the transformed and untransformed E. coli after 1 min- and 2 min-plasma treatment. Furthermore, the enzyme-linked immunosorbent assay (ELISA) and acetyltransferase activity assay showed that the CAT activity was reduced after plasma treatment in both transformed and selected E. coli grown in ampicillin-containing or ampicillin-free medium. Loss of lipopolysaccharide and DNA damage caused by plasma treatment were confirmed by a Limulus test and polymerase chain reaction, respectively. Taken together, these findings suggest the plasma acts to degrade components of the bacteria and is therefore unlikely to display a differential affect against antibiotic-resistant and non-resistant bacteria. Therefore, the plasma method may be useful in eliminating bacteria that are recalcitrant to conventional antibiotic therapy.

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Section: Discussionsupporting

confidence: 93%

Antibiotic-Resistant and Non-Resistant Bacteria Display Similar Susceptibility to Dielectric Barrier Discharge Plasma

Sakudo

Misawa

2020

IJMS

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“…Cold atmospheric plasma (CAP) is a promising technology for medicine, sterilization, and surface and water treatment. CAP has bactericidal, fungicidal, and sporicidal effects, [ 1–4 ] as well as a selective toxic effect on tumor cells. [ 5,6 ] It also stimulates proliferation of keratinocytes [ 7 ] and fibroblasts, [ 8,9 ] which leads to accelerated wound healing.…”

Section: Introductionmentioning

confidence: 99%

Bidirectional mass transfer‐based generation of plasma‐activated water mist with antibacterial properties

Sysolyatina

Lavrikova

Loleyt³

et al. 2020

Plasma Processes & Polymers

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Plasma-activated water mist (PAWM) is obtained by the ignition of plasma within an air-vapor mixture. PAWM demonstrates significant antibacterial properties, decreasing loads of foodborne pathogens by a factor of 35.5 for Listeria monocytogenes, 166 for Salmonella Typhimurium, and 266 for Escherichia coli O157:H7 within 15 s. Bacterial biofilms have a similar species-dependant susceptibility. Biofilms of L. monocytogenes, Salmonella Typhimurium, and E. coli O157:H7 are destroyed by 44%, 77%, and 71%, respectively, after being treated for 2 min. Obtained results suggest importance of short-lived radicals, because PAWM condensate is not bactericidal. A new model of PAW generation as a cyclic process of oxidation reactive nitrogen species by reactive oxygen species, which occurs during effective bidirectional mass transfer between heavily humid air and water mist in plasma discharge, is presented.

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“…Over the past 20 years, various studies have reported the microbicidal effect of plasma including inactivation of prions [ 28 ]. For example, Jalak et al reported the disinfection of scrapie prions by corona discharge in open air [ 29 ], confirming a decrease in both PrP Sc and PrP C by cell infectivity assay and Western blot analysis.…”

Section: Discussionmentioning

confidence: 99%

Effect of Nitrogen Gas Plasma Generated by a Fast-Pulsed Power Supply Using a Static Induction Thyristor on Scrapie Prion

et al. 2020

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Previous studies show that nitrogen gas plasma generated by a fast-pulsed power supply using a static induction thyristor has both virucidal and bactericidal effects. In this study, nitrogen gas plasma was further evaluated for its potential effects on prions, which are well known to be the most resistant pathogen to both chemical and physical inactivation. Aliquots (10 μL) of mouse brain homogenate infected with Chandler scrapie prion were spotted onto cover glasses and subjected to nitrogen gas plasma. Treated samples were recovered and subjected to further analyses. Control prion samples were prepared in exactly the same way but without plasma treatment. Protein misfolding cyclic amplification (PMCA) showed that nitrogen gas plasma treatment at 1.5 kilo pulse per second for 15 or 30 min caused a reduction in the in vitro propagation level of PrPres (proteinase K-resistant prion protein), which was used as an index of abnormal prion protein (PrPSc). Moreover, mice injected with prion treated with plasma for 30 min showed longer survival than mice injected with control prion, indicating that nitrogen gas plasma treatment decreased prion infectivity. Altogether, these results suggest that nitrogen gas plasma treatment can inactivate scrapie prions by decreasing the propagation activity and infectivity of PrPSc.

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Disinfection and Sterilization Using Plasma Technology: Fundamentals and Future Perspectives for Biological Applications

Cited by 235 publications

References 101 publications

Antibiotic-Resistant and Non-Resistant Bacteria Display Similar Susceptibility to Dielectric Barrier Discharge Plasma

Antibiotic-Resistant and Non-Resistant Bacteria Display Similar Susceptibility to Dielectric Barrier Discharge Plasma

Bidirectional mass transfer‐based generation of plasma‐activated water mist with antibacterial properties

Effect of Nitrogen Gas Plasma Generated by a Fast-Pulsed Power Supply Using a Static Induction Thyristor on Scrapie Prion

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