Evaluation of Neonatal Streptozotocin Induced Diabetic Rat Model for the Development of Cataract

Patil, Madhoosudan A.; Suryanarayana, Palla; Putcha, Uday Kumar; Marmamula, Srinivas; Reddy, G. Bhanuprakash

doi:10.1155/2014/463264

Cited by 29 publications

(31 citation statements)

References 49 publications

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“…injection of STZ on the second day of birth at dose level 90 mg kg −1 , developed insulin resistance. 26 In the present study, the extracts showed statistically significant reduction in the blood glucose levels at p < 0.05 on day 30 ( Table 2 ). A statistically significant decrease in the glycated hemoglobin levels was observed at p < 0.05.…”

Section: Resultssupporting

confidence: 56%

Antidiabetic activity of extracts of Anacardium occidentale Linn. leaves on n -streptozotocin diabetic rats

Jaiswal

Tatke

Gabhe

et al. 2017

Journal of Traditional and Complementary Medicine

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Anacardium occidentale L. (Anacardiaceae) is used in South Cameroon as well as in other tropical countries by traditional practitioners as a folk remedy for treatment of diabetes mellitus. We demonstrated the antidiabetic potential of the plant extracts in n-streptozotocin diabetic rats. The aim of the current study was to investigate the antidiabetic effects of ethanol extract of leaves of A. occidentale on neonatal streptozotocin diabetic rats.Two day old neonates were injected with 100 mg/kg of streptozotocin. At the end of the experimental period of 30 days, reduction in the fasting blood glucose levels, serum insulin, glycated hemoglobin levels, serum lipid parameters, and renal function biomarkers were estimated in the control and treated rats. Histopathological examination of liver, kidney and pancreas were also carried out.On administration of 100 mg/kg of plant extract, blood glucose levels of the rats showed 8.01% and 19.25% decrease in the fasting blood glucose levels on day 15 and day 30, respectively. The administration of extract showed that the effects of extract treatment are comparable to treatment with the standard drug Pioglitazone.These results demonstrate significant antidiabetic potential of the ethanol extract of leaves of A. occidentale, justifying the use of plant in the indigenous system of medicine. Further studies for investigating the specific compound(s) responsible for such beneficial role in diabetes would open new outlook in the therapy of type 2 diabetes.

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Section: Resultssupporting

confidence: 56%

Antidiabetic activity of extracts of Anacardium occidentale Linn. leaves on n -streptozotocin diabetic rats

Jaiswal

Tatke

Gabhe

et al. 2017

Journal of Traditional and Complementary Medicine

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“…From the viewpoint of the hepatic L-alanine production, thus, the oral intake of L-glutamine can be regarded as an additional health benefit in the case of type 2 diabetes, which also includes increased glucose uptake and diminished insulin resistance [ 5 , 9 ]. The main characteristics of type 2 diabetes in humans were also detected in the model (neonatal streptozotocin induced diabetic rat) used in the present study, as for example mild fasting hyperglycemy, high insulin levels and some degree of insulin resistance [ 55 , 56 , 57 ]. However, the behavior of livers from type 2 diabetic rats with respect to L-glutamine metabolism was essentially that one observed with livers from healthy rats if one excepts the already mentioned increased L-alanine production.…”

Section: Discussionmentioning

confidence: 91%

The Metabolic Responses to L-Glutamine of Livers from Rats with Diabetes Types 1 and 2

et al. 2016

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There are several claims about the beneficial effects of supplementing L-glutamine to both type 1 and type 2 diabetes. The purpose of the present study was to provide detailed knowledge about the fate of this amino acid in the liver, the first organ that receives the compound when ingested orally. The study was done using the isolated perfused rat liver, an experimental system that preserves the microcirculation of the organ and that allows to measured several parameters during steady-state and pre steady-state conditions. L-Glutamine was infused in the portal vein (5 mM) and several parameters were monitored. Livers from type 1 diabetic rats showed an accelerated response to L-glutamine infusion. In consequence of this accelerated response livers from type 1 diabetic rats presented higher rates of ammonia, urea, glucose and lactate output during the first 25–30 minutes following L-glutamine infusion. As steady-state conditions approached, however, the difference between type 1 diabetes and control livers tended to disappear. Measurement of the glycogen content over a period of 100 minutes revealed that, excepting the initial phase of the L-glutamine infusion, the increased glucose output in livers from type 1 diabetic rats was mainly due to accelerated glycogenolysis. Livers from type 2 diabetic rats behaved similarly to control livers with no accelerated glucose output but with increased L-alanine production. L-Alanine is important for the pancreatic β-cells and from this point of view the oral intake of L-glutamine can be regarded as beneficial. Furthermore, the lack of increased glucose output in livers from type 2 diabetic rats is consistent with observations that even daily L-glutamine doses of 30 g do not increase the glycemic levels in well controlled type 2 diabetes patients.

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“…Other studies have similarly shown that different parameters of oxidative stress and antioxidant defense are impaired in DR. Among these parameters, retinal SOD activity and iPF 2 α levels have been shown to decrease and increase, respectively, in animal models of DR …”

Section: Resultsmentioning

confidence: 99%

Caffeic Acid Alkyl Amide Derivatives Ameliorate Oxidative Stress and Modulate ERK1/2 and AKT Signaling Pathways in a Rat Model of Diabetic Retinopathy

Fathalipour

Eghtedari

Borges

et al. 2019

Chemistry & Biodiversity

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The purpose of this study was to examine the neuroprotective effects of caffeic acid hexyl (CAF6) and dodecyl (CAF12) amide derivatives on the early stage of retinopathy in streptozotocin-induced diabetic rats. Animals were divided in five groups (n = 8/group); one group consisted of non-diabetic rats as control, while the other four were diabetic animals either non-treated or treated with CAF6, CAF12 or resveratrol intravitreally for four weeks. Retinal superoxide dismutase (SOD) activity and 8-iso-prostaglandin F 2α (iPF 2α ) levels were evaluated by an ELISA assay. Phosphorylation of ERK1/2 and AKT was determined by immunoblotting in retinal homogenates. Retinal morphology was also examined using light microscopy. Treatment with CAF6 and CAF12 increased retinal SOD activity, while it decreased iPF 2α levels in diabetic rats. Phosphorylation of ERK1/2 was increased, while AKT phosphorylation was decreased in diabetic rats compared to normal control and these alterations were significantly reversed in diabetic rats treated with CAF6 and CAF12. Furthermore, thickness of the whole retinal layer, outer nuclear layer, and ganglion cell count were decreased in diabetic rats compared to control and CAF6 and CAF12 treatments prevented these changes. CAF6 and CAF12 seem to be effective agents for treatment of diabetic retinopathy via attenuation of retinal oxidative stress and improvement of neuronal survival signaling.

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Evaluation of Neonatal Streptozotocin Induced Diabetic Rat Model for the Development of Cataract

Cited by 29 publications

References 49 publications

Antidiabetic activity of extracts of Anacardium occidentale Linn. leaves on n -streptozotocin diabetic rats

Antidiabetic activity of extracts of Anacardium occidentale Linn. leaves on n -streptozotocin diabetic rats

The Metabolic Responses to L-Glutamine of Livers from Rats with Diabetes Types 1 and 2

Caffeic Acid Alkyl Amide Derivatives Ameliorate Oxidative Stress and Modulate ERK1/2 and AKT Signaling Pathways in a Rat Model of Diabetic Retinopathy

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