Evaluation of N‐acetilglucosaminidase and myeloperoxidase activity in patients with endometriosis‐related infertility undergoing intracytoplasmic sperm injection

Abstract

Infertile patients with endometriosis show a distinct pattern of serum and follicular fluid macrophage/neutrophil activation compared to normally ovulating women undergoing ICSI, which may reflect the role of immune and inflammatory alterations in endometriosis-related infertility.

Identification of local angiogenic and inflammatory markers in the menstrual blood of women with endometriosis

“…Myeloperoxidase (MPO), an enzyme restricted to the azurophil granules of neutrophils, has been extensively used as a marker of polymorphonuclear leukocytes accumulation in tissue samples [25][26][27][28][29][30]. N-acetyl-b-D-glucosaminidase (NAG), present in lysosomes, has been employed to detect macrophage accumulation/activation in a variety of animal and human tissues [26][27][28][29][30][31].…”

Evaluation of Total, Active, and Specific Myeloperoxidase Levels in Women with and without Endometriosis

“…Riley et al [14] found decreased total MPO levels in the peritoneal fluid of stage I endometriosis patients indicating an initially reduced inflammatory neutrophil response, which becomes stronger as the disease progresses. Active MPO levels were found to be decreased in the follicular fluid of endometriosis patients [26]. In menstrual fluid [27] and in peripheral blood [26][27][28], no significant difference was found in active MPO levels between women with and without endometriosis, but no report on total MPO levels was provided.…”

“…Other investigators focused on MPO levels in peritoneal [14], follicular [26], or menstrual [27] fluid, measuring only total MPO levels [14] or MPO activity [26,27] (not specific MPO activity). Riley et al [14] found decreased total MPO levels in the peritoneal fluid of stage I endometriosis patients indicating an initially reduced inflammatory neutrophil response, which becomes stronger as the disease progresses.…”

“…As a marker of inflammation, MPO might serve as a potential noninvasive biomarker of endometriosis [25]. Data regarding the MPO level in peripheral blood of endometriosis patients are relatively scarce and large sample datasets are lacking (n = 17; 38; 58) [26][27][28]. Furthermore, these studies investigated only the activity of MPO, but no total MPO levels [26][27][28].…”

“…Data regarding the MPO level in peripheral blood of endometriosis patients are relatively scarce and large sample datasets are lacking (n = 17; 38; 58) [26][27][28]. Furthermore, these studies investigated only the activity of MPO, but no total MPO levels [26][27][28]. Therefore, our study was done in a large number of subjects (n = 333) to test the hypothesis that total, active, and specific levels of MPO are significantly altered in plasma of women with endometriosis compared to women without endometriosis.…”