Evaluation of Mycoplasma gallisepticum (MG) ts-304 vaccine as a live attenuated vaccine in turkeys

Kanci, Anna; Wijesurendra, Dinidu S; Wawegama, Nadeeka K.; Underwood, Gregory J.; Noormohammadi, Amir H.; Markham, Philip F.; Browning, Glenn F.

doi:10.1016/j.vaccine.2018.02.117

Cited by 17 publications

(13 citation statements)

References 28 publications

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“…Previous studies examining the efficacy and safety of mycoplasma vaccines have focused on assessment of gross and histopathological lesions and evaluation of the adaptive immune response by measuring the antibody response in vaccinated birds after challenge ( 11 , 16 , 22 – 24 , 44 , 45 ). Only a few, recent studies have investigated the genes and/or pathways underlying protection against disease by examining the host responses to vaccination and/or infection with M. gallisepticum ( 26 – 28 ) in order to further understand the pathogenetic processes underlying the pathology and the immune responses to infection with the aim of improving the vaccine development process.…”

Section: Discussionmentioning

confidence: 99%

“…A GapA + clone, strain ts-304, has been isolated from ts-11 and has been shown to be as safe in chickens as the ts-11 strain from which it was derived, and as effective, but at a lower dose ( 21 , 22 ). The ts-304 vaccine has also been shown to colonize the trachea of turkeys and protect against experimental challenge with the M. gallisepticum wild-type strain Ap3AS ( 23 , 24 ).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic Analysis of Long-Term Protective Immunity Induced by Vaccination With Mycoplasma gallisepticum Strain ts-304

et al. 2021

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Live attenuated vaccines are commonly used to control Mycoplasma gallisepticum infections in chickens. M. gallisepticum ts-304 is a novel live attenuated vaccine strain that has been shown to be safe and effective. In this study, the transcriptional profiles of genes in the tracheal mucosa in chickens challenged with the M. gallisepticum wild-type strain Ap3AS at 57 weeks after vaccination with ts-304 were explored and compared with the profiles of unvaccinated chickens that had been challenged with strain Ap3AS, unvaccinated and unchallenged chickens, and vaccinated but unchallenged chickens. At two weeks after challenge, pair-wise comparisons of transcription in vaccinated-only, vaccinated-and-challenged and unvaccinated and unchallenged birds detected no differences. However, the challenged-only birds had significant up-regulation in the transcription of genes and enrichment of gene ontologies, pathways and protein classes involved in infiltration and proliferation of inflammatory cells and immune responses mediated through enhanced cytokine and chemokine production and signaling, while those predicted to be involved in formation and motor movement of cilia and formation of the cellular cytoskeleton were significantly down-regulated. The transcriptional changes associated with the inflammatory response were less severe in these mature birds than in the relatively young birds examined in a previous study. The findings of this study demonstrated that vaccination with the attenuated M. gallisepticum strain ts-304 protects against the transcriptional changes associated with the inflammatory response and pathological changes in the tracheal mucosa caused by infection with M. gallisepticum in chickens for at least 57 weeks after vaccination.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis of Long-Term Protective Immunity Induced by Vaccination With Mycoplasma gallisepticum Strain ts-304

et al. 2021

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“…A live-attenuated vaccine named Vaxsafe MG (strain ts-11) has been reported to provide protection in chickens. The study proved that the vaccine can also be used in turkeys and found safe and efficacious in 3-wk-old turkeys ( Kanci et al., 2018 ). While, limited information is available about the use of Mycoplasma gallinarum as a vector and host, and therefore, further studies are encouraged to scrutinize the organism's genetic system in detail.…”

Section: Mg Vaccine Candidatesmentioning

confidence: 83%

“…The pathogen primarily infects chickens and turkeys ( Ley, 2003 ), but also some other birds, such as house and wild finches ( Ley et al., 1996 ; Dhondt et al., 2014 ) and geese ( Benoina et al., 1988 ). It is well documented that MG is the causative agent of chronic respiratory disease in chickens and infectious sinusitis in turkeys ( Chin et al., 1991 ; Cecchini et al., 2007 ; Wijesurendra et al., 2015 ; Beaudet et al., 2017 ; Kanci et al., 2018 ). The disease is often slowly developed and accompanied with severe inflammation in the respiratory tract of birds ( Harry and Yoder, 1990 ).…”

Section: Introductionmentioning

confidence: 99%

Current status of vaccine research, development, and challenges of vaccines for Mycoplasma gallisepticum

Ishfaq

Khan

et al. 2020

Poultry Science

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Mycoplasma gallisepticum ( MG ) is an important avian pathogen that causes significant economic losses in the poultry industry. Surprisingly, the limited protection and adverse reactions caused by the vaccines, including live vaccines, bacterin-based (killed) vaccines, and recombinant viral vaccines is still a major concern. Mycoplasma gallisepticum strains vary in infectivity and virulence and infection may sometimes unapparent and goes undetected. Although extensive research has been carried out on the biology of this pathogen, information is lacking about the type of immune response that confers protection and selection of appropriate protective antigens and adjuvants. Regardless of numerous efforts focused on the development of safe and effective vaccine for the control of MG, the use of modern DNA vaccine technology selected in silico approaches for the use of conserved recombinant proteins may be a better choice for the preparation of novel effective vaccines. More research is needed to characterize and elucidate MG products modulating MG-host interactions. These products could be used as a reference for the preparation and development of vaccines to control MG infections in poultry flocks.

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“…Three main live attenuated MG vaccine strains have been developed and commercially available: ts-11 (TS-11 ® , Boehringer Ingelheim Animal Health Italia), 6/85 (Nobilis ® Mg 6/85, MSD Animal Health), and F-strain (Cevac ® MG F, Ceva Santé Animale). They have shown high efficacy in controlling the spread of MG in chickens but were not demonstrated to be useful in turkey protection [7,8]. Moreover, F-strain is not recommended for use because of its possible virulence in broilers and turkeys [4,9].…”

Section: Introductionmentioning

confidence: 99%

Molecular Differentiation of Mycoplasma gallisepticum Outbreaks: A Last Decade Study on Italian Farms Using GTS and MLST

et al. 2020

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Mycoplasma gallisepticum (MG) infects many avian species and leads to significant economic losses in the poultry industry. Transmission of this pathogen occurs both horizontally and vertically, and strategies to avoid the spread of MG rely on vaccination and the application of biosecurity measures to maintain breeder groups as pathogen-free. Two live attenuated MG vaccine strains are licensed in Italy: 6/85 and ts-11. After their introduction, the implementation of adequate genotyping tools became necessary to distinguish between field and vaccine strains and to guarantee proper infection monitoring activity. In this study, 40 Italian MG isolates collected between 2010–2019 from both vaccinated and unvaccinated farms were genotyped using gene-targeted sequencing (GTS) of the cythadesin gene mgc2 and multilocus sequence typing (MLST) based on six housekeeping genes. The discriminatory power of GTS typing ensures 6/85-like strain identification, but the technique does not allow the identification ts-11 strains; conversely, MLST differentiates both vaccine strains, describing more detailed interrelation structures. Our study describes MG genetic scenario within a mixed farming context. In conclusion, the use of adequate typing methods is essential to understand the evolutionary dynamics of MG strains in a particular area and to conduct epidemiological investigations in the avian population.

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Evaluation of Mycoplasma gallisepticum (MG) ts-304 vaccine as a live attenuated vaccine in turkeys

Cited by 17 publications

References 28 publications

Transcriptomic Analysis of Long-Term Protective Immunity Induced by Vaccination With Mycoplasma gallisepticum Strain ts-304

Transcriptomic Analysis of Long-Term Protective Immunity Induced by Vaccination With Mycoplasma gallisepticum Strain ts-304

Current status of vaccine research, development, and challenges of vaccines for Mycoplasma gallisepticum

Molecular Differentiation of Mycoplasma gallisepticum Outbreaks: A Last Decade Study on Italian Farms Using GTS and MLST

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