Evaluation of mycophenolic acid production by Penicillium brevicompactum MUCL 19011 in batch and continuous submerged cultures

Ardestani, Fatemeh; Fatemi, Seyed Safa-Ali; Yakhchali, Bagher; Hosseyni, Seyed Morteza; Najafpour, Ghasem

doi:10.1016/j.bej.2010.03.008

Cited by 30 publications

(28 citation statements)

References 21 publications

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“…A stock culture of P. brevicompactum ATCC 16024 was prepared as slants containing potato dextrose agar (PDA) at pH 6.5, sterilized at 120 °C for 15 min and incubated at 27 °C for one week, then, stored at 4 °C [18].…”

Section: Microorganism and Inoculummentioning

confidence: 99%

“…Then, spores were shaved and extracted with a sterile loop under aseptic conditions and dispersed in distilled water. The number of spores in inoculum were counted with a Thoma lam and adjusted to 10 -10 spores per mL [18]. 7 8 conditions and also estimation of performance at the In Taguchi method, orthogonal arrays are used to to reduce experimental errors and to enhance the efficiency and reproducibility of laboratory experiments [29].…”

Section: Microorganism and Inoculummentioning

confidence: 99%

“…A rotary shaker incubator (JAHL-JSH 20LUR, Iran) was used for this reason. After inoculation with 0.5 mL of the spore suspension (~5×10 7 cell per mL) under aseptic conditions, shake flasks were incubated at 27 °C for 300 h [18].…”

Section: Microorganism and Inoculummentioning

confidence: 99%

“…lymphocytes cells, which consequently stops cell Mycophenolic acid [6-(4-hydroxy-6-methoxy-7-Penicillium especially P. brevicompactum [18][19][20][21][22][23][24][25][26], methyl-3-oxophthalanyl)-4-methyl-4-hexenic acid MPA] as Penicillium stoloniferum [27] and Penicillium roqueforti an antibiotic substance for bacteria, fungi and viruses [1-[28] can produce MPA as a secondary metabolite by 3] is produced by several species of Penicillium [4]. MPA submerged and solid-state fermentation processes.…”

Section: Introductionmentioning

confidence: 99%

“…MPA also has been showed a high anti-HIV activity and was production processes have been done in batch [18][19][20][21][22][23][24][25][26][27][28], introduced as a new strategy to overcome on HIV in repeated batch with immobilized cells [20] and continuous human body [5,6]. In addition, MPA and some of its [18] modes. Process optimization for different cell derivatives such as mycophenolate mofetil (MMF) and products was done in some previous researches [29 -30].…”

Section: Introductionmentioning

confidence: 99%

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Production of Mycophenolic Acid by Penicillium brevicompactum in a Submerged Batch Culture: Optimization of Culture Conditions Using Taguchi Approach

Ardestani¹

2013

IJEE

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Mycophenolic acid (MPA) is an antibiotic and immunosuppressive agent, as a secondary metabolite of Penicillium brevicompactum. Parameter optimization of culture composition using Test plan L16, available in the form of an orthogonal array and software for automatic design and analysis of the experiments, both based on Taguchi approach was performed in MAP production process by Penicillium brevicompactum ATCC 16024 in a batch submerged culture. Optimal levels of key parameters including glucose, enzymaticaly hydrolyzed casein, methionine and glycine concentrations were determined. Optimum glucose, enzymaticaly hydrolyzed casein, methionine and glycine concentrations were obtained 80, 25, 1.2 and 20 g. L , respectively.

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Section: Microorganism and Inoculummentioning

confidence: 99%

Section: Microorganism and Inoculummentioning

confidence: 99%

Section: Microorganism and Inoculummentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Production of Mycophenolic Acid by Penicillium brevicompactum in a Submerged Batch Culture: Optimization of Culture Conditions Using Taguchi Approach

Ardestani¹

2013

IJEE

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Unlocking the biosynthetic potential of Penicillium roqueforti for hyperproduction of the immunosuppressant mycophenolic acid: Gamma radiation mutagenesis and response surface optimization of fermentation medium

El‐Sayed

Zaki

2022

Biotech and App Biochem

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Based on the broad clinical utility of the immunosuppressant mycophenolic acid (MPA), this article aims to intensify the biosynthetic potential of Penicillium roqueforti for more effective hyperproduction of the drug. Several mutants were generated from irradiation mutagenesis and screened. Two strains (GM1013 and GM1093) presented an elevated MPA productivity with significant yield constancy over 10 subsequent generations. By investigating the effect of some phosphorous sources and mineral salts on MPA production by the two mutants, KH 2 PO 4 and FeSO 4 ⋅7H 2 O were most preferred by the two mutants for higher MPA production rates. Statistics-dependent experimental designs were also employed for optimizing medium components for maximum MPA production.Medium components were primarily screened using the Plackett-Burman model to demonstrate the most important components that most significantly affect MPA production. The concentrations of these significant components were then optimized through a central composite rotatable model. In conclusion, gammaradiation mutation and response surface optimization resulted in a promising MPA productivity by P. roqueforti GM1013. To our knowledge, the MPA-yield achieved in this study (2933.32 mg L -1 ) is the highest reported by academic laboratories from P. roqueforti cultures, which could be of economic value for a prospective large industrialized application.

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Production of Mycophenolic Acid by a Newly Isolated Indigenous Penicillium glabrum

Mahmoudian¹,

Sharifirad²,

Yakhchali³

et al. 2021

Curr Microbiol

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Soil-occupant fungi produce a variety of mycotoxins as secondary metabolites, one of which is mycophenolic acid (MPA), an antibiotic and immunosuppressive agent. MPA is mainly produced by several species of Penicillium, especially Penicillium brevicompactum. Here, we present the first report of MPA production by a local strain belonging to Penicillium glabrum species. We screened ascomycete cultures isolated from moldy food and fruits, as well as soils, collected from different parts of Iran. MPA production of one hundred and forty Penicillium isolates was analyzed using HPLC. Three MPA producer isolates were identified, among which the most producer was subjected to further characterization, based on morphological and microscopic analysis, as well as molecular approach (ITS, rDNA and beta-tubulin gene sequences). The results revealed that the best MPA producer belongs to P. glabrum IBRC-M 30518, and can produce 1079 mg/L MPA in Czapek-Dox medium.

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Evaluation of mycophenolic acid production by Penicillium brevicompactum MUCL 19011 in batch and continuous submerged cultures

Cited by 30 publications

References 21 publications

Production of Mycophenolic Acid by Penicillium brevicompactum in a Submerged Batch Culture: Optimization of Culture Conditions Using Taguchi Approach

Production of Mycophenolic Acid by Penicillium brevicompactum in a Submerged Batch Culture: Optimization of Culture Conditions Using Taguchi Approach

Unlocking the biosynthetic potential of Penicillium roqueforti for hyperproduction of the immunosuppressant mycophenolic acid: Gamma radiation mutagenesis and response surface optimization of fermentation medium

Production of Mycophenolic Acid by a Newly Isolated Indigenous Penicillium glabrum

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