Unlocking the biosynthetic potential of <i>Penicillium roqueforti</i> for hyperproduction of the immunosuppressant mycophenolic acid: Gamma radiation mutagenesis and response surface optimization of fermentation medium

El‐Sayed, El‐Sayed R.; Zaki, Amira G

doi:10.1002/bab.2353

Cited by 12 publications

(7 citation statements)

References 47 publications

(84 reference statements)

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“…Also, the same irradiation dose improved production of the cardiac glycoside digoxin to a five-fold increase by Epicoccum nigrum (El-Sayed et al 2020b ). Moreover, the same effect of exposure to gamma rays on the survival and the fungal growth was observed by previous reports (Awan et al 2011 ; El-Sayed and Zaki 2022 ; El-Sayed et al 2019a ; b ; c ). Previous reports concluded the effect of gamma rays as an energetic ionizing radiation in induction of mutations in the exposed cells (Thacker 1999 ) through the repair mechanism in genes of the DNA of the irradiated cells (Ismaiel et al 2014 , 2015 ; Zaki et al 2020 ; Zaki and El-Sayed 2021 ).…”

Section: Discussionsupporting

confidence: 83%

Bioprospecting endophytic fungi for antifeedants and larvicides and their enhancement by gamma irradiation

et al. 2022

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The search and discovery of new natural products with antifeedant and larvicidal potentials to mitigate harmful insects are scientific pressing issues in the modern agriculture. In this paper, the antifeedant and larvicidal potentials of 69 fungal isolates were screened against the Egyptian cotton leafworm Spodoptera littoralis. A total of 17 isolates showed the insecticidal potentials with three promising isolates. These strains were Aspergillus sydowii, Lasiodiplodia theobromae, and Aspergillus flavus isolated from Ricinus communis (bark), Terminalia arjuna (Bark), and Psidium guajava (twigs), respectively. The effect of gamma irradiation on the antifeedant and larvicidal activities of the three strains was investigated. Exposure of the fungal spores to 1000 Gy of gamma rays significantly intensified both the antifeedant and larvicidal potentials. To identify compounds responsible for these activities, extracts of the three strains were fractionated by thin layer chromatography. The nature of the separated compounds namely, Penitrem A, 1, 3, 5, 8- tetramethyl- 4, 6-diethyl- 7- [2- (methoxycarbonyl)ethyl] porphyrin (from A. sydowii), Penitrem A, 2, 7, 12, 17-Tetramethyl-3, 5:8, 10:13, 15:18, 20-tetrakis (2,2-dimethylpropano) porphyrin (from A. flavus), N,N-Diethyl-3-nitrobenzamide, and Diisooctyl-phthalate (from L. theobromae) were studied by GC-MS analysis. These findings recommend endophytic fungi as promising sources of novel natural compounds to mitigate harmful insects.

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Section: Discussionsupporting

confidence: 83%

Bioprospecting endophytic fungi for antifeedants and larvicides and their enhancement by gamma irradiation

et al. 2022

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“…In generally, the observed increase in the prepared ZnONPs yield could be mainly attributed to the gamma ray’s exposure of the fungal cells prior to the preparation of the cell-free filtrate used in the preparation of NPs. Exposure of the fungus cells to gamma rays might induced mutations (El-Sayed et al 2022a ; Hazaa et al 2022 ) in these cells resulting in the over-production of all the active metabolites and enzymes responsible (El-Sayed et al 2022c ; El-Sayed and Zaki 2022 ) for the bio-reduction of zinc sulfate salt and intensifying the formation of high concentrations of ZnONPs. Previous reports showed that the irradiation by gamma rays at some specific doses was successfully used for enhancing the production of various fungal metabolites (El-Sayed 2021 ; Zaki and El-Sayed 2021 ; El-Sayed et al 2019a , b , c ; El-Sayed et al 2020c , d , e , f ).…”

Section: Discussionmentioning

confidence: 99%

Improving carboxymethyl cellulose edible coating using ZnO nanoparticles from irradiated Alternaria tenuissima

et al. 2022

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In this paper, gamma-irradiation was successfully used to intensify the yield of Zinc oxide nanoparticles (ZnONPs) produced by the fungus Alternariatenuissima as a sustainable and green process. The obtained data showed that 500 Gy of gamma-irradiation increased ZnONPs’ yield to approximately four-fold. The synthesized ZnONPs were then exploited to develop active Carboxymethyl Cellulose films by casting method at two different concentration of ZnONPs 0.5% and 1.0%. The physicochemical, mechanical, antioxidant, and antimicrobial properties of the prepared films were evaluated. The incorporation of ZnONPs in the Carboxymethyl Cellulose films had significantly decreased solubility (from 78.31% to 66.04% and 59.72%), water vapor permeability (from 0.475 g m−2 to 0.093 g m−2 and 0.026 g m−2), and oxygen transfer rate (from 24.7 × 10–2 to 2.3 × 10–2 and 1.8 × 10–2) of the respective prepared films. Meanwhile, tensile strength (from 183.2 MPa to 203.34 MPa and 235.94 MPa), elongation (from 13.0% to 62.5% and 83.7%), and Yang's modulus (from 325.344 to 1410.0 and 1814.96 MPa) of these films were increased. Moreover, the antioxidant and antimicrobial activities against several human and plant pathogens the prepared of Carboxymethyl Cellulose-ZnONPs films were significantly increased. In conclusion, the prepared Carboxymethyl Cellulose-ZnONPs films showed enhanced activities in comparison with Carboxymethyl Cellulose film without NPs. With these advantages, the fabricated Carboxymethyl Cellulose-ZnONPs films in this study could be effectively utilized as protective edible coating films of food products.

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“…In general, fungi-mediated synthesis of several nanomaterials have magnificently emerged as the most advantageous (Grasso et al 2021 ). Over the years, fungi have been employed extensively in several industrial applications (Dorcheh and Vahabi 2016 ; El-Sayed and Zaki 2022 ) as the most efficient biotechnological factories due to their flexibility, simplicity, easy maintenance, and tolerance. In this regard, the use of endophytes has emerged as a new research area for green and cost-effective production of several NPs.…”

Section: Introductionmentioning

confidence: 99%

Harnessing endophytic fungi for biosynthesis of selenium nanoparticles and exploring their bioactivities

et al. 2022

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In the light of the fast growing several applications of selenium nanoparticles (SeNPs) in different industrial and agricultural sectors, this paper was conducted to explore the suitability of endophytic fungi as nano-factories for SeNPs. Thus, 75 fungal isolates were recovered from plant tissues and tested for their efficacy to biosynthesize SeNPs. Four promising strains were found able to synthesis SeNPs with different characteristics and identified. These strains were Aspergillus quadrilineatus isolated from the twigs of Ricinus communis, Aspergillus ochraceus isolated from the leaves of Ricinus communis, Aspergillus terreus isolated from the twigs of Azadirachta indica, and Fusarium equiseti isolated from the twigs of Hibiscus rose-sinensis. The synthesized SeNPs were characterized by several techniques viz., UV–Vis, X-ray diffraction, Dynamic light scattering analyses, High resolution transmission electron microscopy, and Fourier transform infrared spectroscopy, to study their crystalline structure, particle sized distribution, and morphology. Furthermore, the in vitro antimicrobial and antioxidant activities were evaluated. SeNPs synthesized by the four strains showed potent antifungal and antibacterial potentials against different human and phyto- pathogens. Moreover, SeNPs synthesized by the respective strains showed promising antioxidant power with IC50 values of 198.32, 151.23, 100.31, and 91.52 µg mL− 1. To the best of our knowledge, this is the first study on the use of endophytic fungi for SeNPs’ biosynthesis. The presented research recommends the use of endophytic fungi as facile one-pot production bio-factories of SeNPs with promising characteristics.

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Unlocking the biosynthetic potential of Penicillium roqueforti for hyperproduction of the immunosuppressant mycophenolic acid: Gamma radiation mutagenesis and response surface optimization of fermentation medium

Cited by 12 publications

References 47 publications

Bioprospecting endophytic fungi for antifeedants and larvicides and their enhancement by gamma irradiation

Bioprospecting endophytic fungi for antifeedants and larvicides and their enhancement by gamma irradiation

Improving carboxymethyl cellulose edible coating using ZnO nanoparticles from irradiated Alternaria tenuissima

Harnessing endophytic fungi for biosynthesis of selenium nanoparticles and exploring their bioactivities

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