Evaluation of Mycobacterium tuberculosis-specific antibody responses for the discrimination of active and latent tuberculosis infection

Wang, Sen; Wu, Jing; Chen, Jiazhen; Gao, Yan; Shu, Zhang; Zhou, Zumo; Huang, Heqing; Shao, Lingyun; Jin, Jialin; Zhang, Ying; Zhang, Wenhong

doi:10.1016/j.ijid.2018.01.007

Cited by 23 publications

(19 citation statements)

References 36 publications

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“…Alterations in disease-specific IgG properties including antigen specific titers (19,20), isotype switching (21), and glycosylation (22)(23)(24)(25)(26) provide insights into disease relapse or severity across diseases ranging from cancer (27,28), autoimmunity (29,30), and infections (20,31). While changes in disease-specific titers do not always reflect changes in disease activity (32)(33)(34)(35), alterations in disease-specific Fc-properties provide critical qualitative insights into disease activity. Given that changes in IgG Fc-profiles also track with altered Fc-effector function, unique humoral markers of disease activity may also provide additional insights into the mechanism(s) of enhanced disease control and even elimination.…”

Section: Introductionmentioning

confidence: 99%

Antibody Subclass and Glycosylation Shift Following Effective TB Treatment

Grace

Dolatshahi

et al. 2021

Front. Immunol.

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With an estimated 25% of the global population infected with Mycobacterium tuberculosis (Mtb), tuberculosis (TB) remains a leading cause of death by infectious diseases. Humoral immunity following TB treatment is largely uncharacterized, and antibody profiling could provide insights into disease resolution. Here we focused on the distinctive TB-specific serum antibody features in active TB disease (ATB) and compared them with latent TB infection (LTBI) or treated ATB (txATB). As expected, di-galactosylated glycan structures (lacking sialic acid) found on IgG-Fc differentiated LTBI from ATB, but also discriminated txATB from ATB. Moreover, TB-specific IgG4 emerged as a novel antibody feature that correlated with active disease, elevated in ATB, but significantly diminished after therapy. These findings highlight 2 novel TB-specific antibody changes that track with the resolution of TB and may provide key insights to guide TB therapy.

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Section: Introductionmentioning

confidence: 99%

Antibody Subclass and Glycosylation Shift Following Effective TB Treatment

Grace

Dolatshahi

et al. 2021

Front. Immunol.

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“…The ELISA method is simple and fast, and is widely used in the serological diagnosis of tuberculosis (23)(24)(25). Thus far, however, the sensitivity and specificity of the antigens used have been poor and need to be further improved (26)(27)(28)(29)(30). The 16kD protein (heat shock protein HspX, Rv2031c), as a small molecule heat shock protein, has strong species specificity, and, as a membrane protein, is anchored on the cell membrane and has strong immunogenicity (31,32).…”

Section: Introductionmentioning

confidence: 99%

Characterization of antibody response against 16kD and 38kD of M. tuberculosis in the assisted diagnosis of active pulmonary tuberculosis

Hao¹,

Bai²,

Ding³

et al. 2020

Ann Transl Med

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Background: In view of the inability of traditional etiological methods to diagnose pulmonary tuberculosis rapidly and effectively, the antibody responses against 38kD and 16kD-antigens of Mycobacterium tuberculosis (M. tuberculosis) were both detected in order to obtain a better serological detection method for M. tuberculosis.Methods: M. tuberculosis-secreted protein 38kD and membrane protein 16kD were prokaryotically expressed and purified, and then used as detection antigens. A novel evolved immunoglobulin-binding molecule (NEIBM)-ELISA method was used to detect antibody levels against 38kD and 16kD in active tuberculosis patients (confirmed tuberculosis cases and clinically diagnosed cases), to explore the significance of these two antigens in serological detection of M. tuberculosis, and to study the diagnostic value of the combined detection of the two antigens in active pulmonary tuberculosis. Results:The results showed that the positive detection rates of the 16kD antigen and 38kD antigen of M. tuberculosis were higher (about 44%) in the confirmed cases of tuberculosis, and there was no significant difference in the positive detection rates of the two antigens (P=0.786). The combined detection of these two antigens showed that the positive detection rate could be increased to 61.5%, which was significantly better than the detection effect of the two antigens alone. The positive detection rates of 16kD and 38kD antigens were 26-30% in clinically diagnosed tuberculosis cases, which were lower than those in confirmed tuberculosis cases, and there was no significant difference in the positive detection rates of the two antigens (P=0.242). The detection effect of the two combined antigens was better than that of the 16kD and 38kD antigens alone, but the detection rate was still lower than that of the confirmed tuberculosis cases.Conclusions: This study found that the detection effect of 16kD and 38kD antigens was similar in confirmed cases and clinically diagnosed cases of pulmonary tuberculosis, and that the detection effect needs to be further improved. The combined detection of the two antigens showed a significantly better detection effect than the two antigens alone, suggesting that the combined detection of multiple antigens can be used for serological diagnosis of M. tuberculosis infection in clinic.

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“…Serological tests that have been developed for the market so far ( Table 2 ) include the Anda TB test, IBL test, Pathozyme-Myco IgG test (Myco G), and TB complex test ( 36 ). While these approaches have yielded promising results, there are no approved tests in the market yet due to their poor sensitivity and specificity ranging between 57 and 94% ( 37 , 38 ).…”

Section: Resultsmentioning

confidence: 99%

Past and Present Approaches to Diagnosis of Active Pulmonary Tuberculosis

2021

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Tuberculosis disease continues to contribute to the mortality burden globally. Due to the several shortcomings of the available diagnostic methods, tuberculosis disease continues to spread. The difficulty to obtain sputum among the very ill patients and the children also affects the quick diagnosis of tuberculosis disease. These challenges warrant investigating different sample types that can provide results in a short time. Highlighted in this review are the approved pulmonary tuberculosis diagnostic methods and ongoing research to improve its diagnosis. We used the PRISMA guidelines for systematic reviews to search for studies that met the selection criteria for this review. In this review we found out that enormous biosignature research is ongoing to identify host biomarkers that can be used as predictors of active PTB disease. On top of this, more research was also being done to improve already existing diagnostic tests. Host markers required more optimization for use in different settings given their varying sensitivity and specificity in PTB endemic and non-endemic settings.

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Evaluation of Mycobacterium tuberculosis-specific antibody responses for the discrimination of active and latent tuberculosis infection

Cited by 23 publications

References 36 publications

Antibody Subclass and Glycosylation Shift Following Effective TB Treatment

Antibody Subclass and Glycosylation Shift Following Effective TB Treatment

Characterization of antibody response against 16kD and 38kD of M. tuberculosis in the assisted diagnosis of active pulmonary tuberculosis

Past and Present Approaches to Diagnosis of Active Pulmonary Tuberculosis

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