Evaluation of multiplex assay platforms for detection of influenza hemagglutinin subtype specific antibody responses

Li, Zhu-Nan; Weber, Kimberly M.; Limmer, Rebecca; Horne, Bobbi Jo; Stevens, James; Schwerzmann, Joy; Wrammert, Jens; McCausland, Megan; Phipps, Andrew J.; Hancock, Kathy; Jernigan, Daniel B.; Levine, Min Z.; Katz, Jacqueline M.; Miller, Joseph D.

doi:10.1016/j.jviromet.2017.01.008

Cited by 11 publications

(14 citation statements)

References 29 publications

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“…Typically ≥ 4‐fold rise in HI and/or MN titers from paired sera collection indicates a positive antibody response to recent influenza infection or vaccination . In the multiplex MAGPIX assay, previously we used ≥ 2‐fold rise in MFI as the cutoff to determine serologic responses from paired sera, and the influenza viruses with the highest fold rise in MFI correlated with viruses of potential exposure . In the current study, no seroconversions (≥4‐fold rise) were detected by HI/MN for paired sera collected from A(H5N1) study.…”

Section: Resultsmentioning

confidence: 56%

Identification of novel influenza A virus exposures by an improved high‐throughput multiplex MAGPIX platform and serum adsorption

Cheng

Poirot

et al. 2019

Influenza Resp Viruses

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Background The development of serologic assays that can rapidly assess human exposure to novel influenza viruses remains a public health need. Previously, we developed an 11‐plex magnetic fluorescence microsphere immunoassay (MAGPIX) by using globular head domain recombinant hemagglutinins (rHAs) with serum adsorption using two ectodomain rHAs. Methods We compared sera collected from two cohorts with novel influenza exposures: animal shelter staff during an A(H7N2) outbreak in New York City in 2016‐2017 (n = 119 single sera) and poultry workers from a live bird market in Bangladesh in 2012‐2014 (n = 29 pairs). Sera were analyzed by microneutralization (MN) assay and a 20‐plex MAGPIX assay with rHAs from 19 influenza strains (11 subtypes) combined with serum adsorption using 8 rHAs from A(H1N1) and A(H3N2) viruses. Antibody responses were analyzed to determine the novel influenza virus exposure. Results Among persons with novel influenza virus exposures, the median fluorescence intensity (MFI) against the novel rHA from exposed influenza virus had the highest correlation with MN titers to the same viruses and could be confirmed by removal of cross‐reactivity from seasonal H1/H3 rHAs following serum adsorption. Interestingly, in persons with exposures to novel influenza viruses, age and MFIs against exposed novel HA were negatively correlated, whereas in persons without exposure to novel influenza viruses, age and MFI against novel HAs were positively correlated. Conclusions This 20‐plex high‐throughput assay with serum adsorption will be a useful tool to detect novel influenza virus infections during influenza outbreak investigations and surveillance, especially when well‐paired serum samples are not available.

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Section: Resultsmentioning

confidence: 56%

Identification of novel influenza A virus exposures by an improved high‐throughput multiplex MAGPIX platform and serum adsorption

Cheng

Poirot

et al. 2019

Influenza Resp Viruses

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“…The reliability and high-throughput of BLI platforms can assist in a multitude of vaccine research and immunotherapeutics applications. Recently, Li et al [ 25 ] found its use for serologic surveys and seroepidemiologic investigations for the identification of influenza hemagglutinin (HA) subtypes specific antibody responses. The influenza A virus HA glycoprotein is important during initial stages of infection, in which it is responsible for binding the virus to sialic acid present on the host cell membrane [ 26 ].…”

Section: Antibody Design and Antibody Capture Strategiesmentioning

confidence: 99%

“…Traditional serological surveys are performed by hemagglutination inhibition (HI) and virus microneutralization (MN) assays [ 27 , 28 ]. When evaluating potential new technologies, Li et al [ 25 ] found that BLI presents high sensitivity and expected specificity for human sera from patients exposed or unexposed to heterologous subtype influenza HA.…”

Section: Antibody Design and Antibody Capture Strategiesmentioning

confidence: 99%

“…Sources are peer-reviewed publications identified through Science Direct and Google Scholar. Disease Organism Citation n# Selected Reference HIV Lentivirus, virus 53 [ 29 ] Flu/Influenza Orthomyxoviridae, virus 36 [ 25 ] Ebola virus Filoviridae, virus 8 [ 24 ] Dengue Flavivirus, virus 6 [ 37 ] Smallpox, variola Vaccinia virus (VACV) orthopoxvirus, virus 6 [ 6 ] Staph infection Staphylococcus aureus , bacteria 5 [ 38 ] Malaria Plasmodium falciparum , protozoa 4 [ 39 ] Chikungunya infection Chikungunya (CHIKV), virus 3 [ 30 ] Tuberculosis Mycobacterium tuberculosis , bacteria …”

Section: Table A1mentioning

confidence: 99%

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Strategies Using Bio-Layer Interferometry Biosensor Technology for Vaccine Research and Development

Petersen¹

2017

Biosensors

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Bio-layer interferometry (BLI) real-time, label-free technology has greatly contributed to advances in vaccine research and development. BLI Octet platforms offer high-throughput, ease of use, reliability, and high precision analysis when compared with common labeling techniques. Many different strategies have been used to immobilize the pathogen or host molecules on BLI biosensors for real-time kinetics and affinity analysis, quantification, or high-throughput titer. These strategies can be used in multiple applications and shed light onto the structural and functional aspects molecules play during pathogen-host interactions. They also provide crucial information on how to achieve protection. This review summarizes some key BLI strategies used in human vaccine research and development.

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“…From serological tests, the most commonly used are hemagglutination inhibition assay (HAI), microneutralization/virus neutralization assay (VN), single radial hemolysis (SRH), complement fixation assay, enzyme-linked immunosorbent assay (ELISA) and Western blotting [55]. These kinds of tests are generally not recommended because of paired serum samples necessity.…”

Section: Influenza Virus Conventional Detection Methodsmentioning

confidence: 99%

Detection Methods of Human and Animal Influenza Virus—Current Trends

Dziąbowska¹,

Czaczyk²,

Nidzworski³

2018

Biosensors

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The basic affairs connected to the influenza virus were reviewed in the article, highlighting the newest trends in its diagnostic methods. Awareness of the threat of influenza arises from its ability to spread and cause a pandemic. The undiagnosed and untreated viral infection can have a fatal effect on humans. Thus, the early detection seems pivotal for an accurate treatment, when vaccines and other contemporary prevention methods are not faultless. Public health is being attacked with influenza containing new genes from a genetic assortment between animals and humankind. Unfortunately, the population does not have immunity for mutant genes and is attacked in every viral outbreak season. For these reasons, fast and accurate devices are in high demand. As currently used methods like Rapid Influenza Diagnostic Tests lack specificity, time and cost-savings, new methods are being developed. In the article, various novel detection methods, such as electrical and optical were compared. Different viral elements used as detection targets and analysis parameters, such as sensitivity and specificity, were presented and discussed.

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Evaluation of multiplex assay platforms for detection of influenza hemagglutinin subtype specific antibody responses

Cited by 11 publications

References 29 publications

Identification of novel influenza A virus exposures by an improved high‐throughput multiplex MAGPIX platform and serum adsorption

Identification of novel influenza A virus exposures by an improved high‐throughput multiplex MAGPIX platform and serum adsorption

Strategies Using Bio-Layer Interferometry Biosensor Technology for Vaccine Research and Development

Detection Methods of Human and Animal Influenza Virus—Current Trends

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