Evaluation of Multiple-Quantum-Filtered23Na NMR in Monitoring Intracellular Na Content in the Isolated Perfused Rat Heart in the Absence of a Chemical-Shift Reagent

Tauskela, Joseph S.; Dizon, José; Whang, John; Katz, José

doi:10.1006/jmre.1997.1181

Cited by 35 publications

(48 citation statements)

References 39 publications

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“…1996; Tauskela et al. 1997). In our situation, the small decrease in TQF signal that was observed during the 15 min ischemia is contraintuitive at first sight.…”

Section: Discussionmentioning

confidence: 99%

Acute changes in extracellular volume fraction in skeletal muscle monitored by²³Na NMR spectroscopy

2017

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In this article, we induced acute changes in extracellular volume fraction in skeletal muscle tissue and compared the sensitivity of a standard 1H T2 imaging method with different 23Na‐NMR spectroscopy parameters within acquisition times compatible with clinical investigations. First, we analyzed the effect of a short ischemia on the sodium distribution in the skeletal muscle. Then, the lower leg of 21 healthy volunteers was scanned under different vascular filling conditions (vascular draining, filling, and normal condition) expected to modify exclusively the extracellular volume. The first experiment showed no change in the total sodium content during a 15 min ischemia, but the intracellular weighted 23Na signal slowly decreased. For the second part, significant variations of total sodium content, sodium distribution, and T1 and T2∗ of 23Na signal were observed between different vascular filling conditions. The measured sodium distribution correlates significantly with sodium T1 and with the short and long T2∗ fractions. In contrast, significant changes in the proton T2w signal were observed only in three muscles. Altogether, the mean T2w signal intensity of all muscles as well as their mean T2 did not vary significantly with the extracellular volume changes. In conclusion, at the expense of giving up spatial resolution, the proposed 23Na spectroscopic method proved to be more sensitive than standard 1H T2 approach to monitor acute extracellular compartment changes within muscle tissue.

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“…1996; Tauskela et al. 1997). In our situation, the small decrease in TQF signal that was observed during the 15 min ischemia is contraintuitive at first sight.…”

Section: Discussionmentioning

confidence: 99%

Acute changes in extracellular volume fraction in skeletal muscle monitored by²³Na NMR spectroscopy

2017

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“…However, changes in the TQ-filtered Na + i signal of the in situ liver during global ischemia were shown to be different than changes in the SQ Na + i signal (12,25). A similar disparity between SQ and TQ-filtered signals has been seen in brain (27), but not in red blood cells (28) or heart (26). These findings suggest that this phenomenon may be tissue dependent.…”

Section: Introductionmentioning

confidence: 70%

“…It has been demonstrated in the perfused rat heart and in the rat liver in situ that the small but measurable Na + e signal that passes through a triple-quantum (TQ) filter is largely unchanged during ischemia and other physiological perturbations (12,(24)(25)(26). Thus, changes in total TQ-filtered 23 Na signal intensity in the absence of an SR may directly report changes in [Na…”

Section: Introductionmentioning

confidence: 92%

“…Because of the high concentration of macromolecules in the intracellular space, it was proposed that Na should produce a large MQ-filtered signal, while the more aqueous nature of the extracellular spaces should produce very little MQ-filtered signal (21). Studies on human blood samples (22) and various animal models (23)(24)(25)(26), however, have shown a significant contribution of Na + e in MQ-filtered spectra. In comparison to SQ spectra, however, MQ-filtered spectra have a much larger contribution from Na + i .…”

Section: Introductionmentioning

confidence: 97%

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Triple-Quantum-Filtered 23Na NMR Spectroscopy of Subcutaneously Implanted 9L Gliosarcoma in the Rat in the Presence of TmDOTP5−

Winter

Bansal

2001

Journal of Magnetic Resonance

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“…In general, some extracellular sodium signal also passes through an MQ filter (8.9): however, in contrast to the simple SQ experiment, in which the vast majority of observed signal is from extracellular sodium, with an MQ filter a large percentage of the observed signal comes from intracellular sodium (9)(10)(11)(12)(13). In addition, MQ-filtered signal is relatively insensitive to possible changes in extracellular Na+, and changes in MQ-filtered Na+ signal have been correlated to changes in intracellular sodium (9-1 1).…”

mentioning

confidence: 96%

Three‐dimensional triple‐quantum‐filtered ²³Na imaging of the dog head in vivo

Kalyanapuram

Seshan

Bansal

1998

Magnetic Resonance Imaging

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Multiple-quantum (MQ) -filtered 23Na NMR has been proposed as a means to partially discriminate between intracellular and extracellular sodium. However, low signal-to-noise ratio (SNR) has been a major obstacle to MQ-filtered 23Na imaging becoming an important technique for biological and clinical applications. We compared the various MQ-filtered 23Na imaging pulse sequences to select the optimum sequence that provides the best SNR. The results of phantom experiments show that the gradient-echo MQ-filtered 23Na imaging sequence produces the best SNR. We also report, for the first time, three-dimensional single-quantum (SQ) and triple-quantum (TQ) -filtered 23Na images of the live dog brain and demonstrate the sensitivity of these images to ischemia produced by euthanizing the animal. The SQ images showed a 10% to 15% decrease in signal intensity from the brain post-mortem, whereas the TQ-filtered images showed a 40% to 50% increase. These changes in signal intensities are consistent with the influx of Na+ into the cells upon death. The feasibility of obtaining TQ-filtered 23Na images of in situ dog brain encourages us to apply this technique to humans.

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Evaluation of Multiple-Quantum-Filtered23Na NMR in Monitoring Intracellular Na Content in the Isolated Perfused Rat Heart in the Absence of a Chemical-Shift Reagent

Cited by 35 publications

References 39 publications

Acute changes in extracellular volume fraction in skeletal muscle monitored by²³Na NMR spectroscopy

Acute changes in extracellular volume fraction in skeletal muscle monitored by²³Na NMR spectroscopy

Triple-Quantum-Filtered 23Na NMR Spectroscopy of Subcutaneously Implanted 9L Gliosarcoma in the Rat in the Presence of TmDOTP5−

Three‐dimensional triple‐quantum‐filtered ²³Na imaging of the dog head in vivo

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Evaluation of Multiple-Quantum-Filtered23Na NMR in Monitoring Intracellular Na Content in the Isolated Perfused Rat Heart in the Absence of a Chemical-Shift Reagent

Cited by 35 publications

References 39 publications

Acute changes in extracellular volume fraction in skeletal muscle monitored by23Na NMR spectroscopy

Acute changes in extracellular volume fraction in skeletal muscle monitored by23Na NMR spectroscopy

Triple-Quantum-Filtered 23Na NMR Spectroscopy of Subcutaneously Implanted 9L Gliosarcoma in the Rat in the Presence of TmDOTP5−

Three‐dimensional triple‐quantum‐filtered 23Na imaging of the dog head in vivo

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Acute changes in extracellular volume fraction in skeletal muscle monitored by²³Na NMR spectroscopy

Acute changes in extracellular volume fraction in skeletal muscle monitored by²³Na NMR spectroscopy

Three‐dimensional triple‐quantum‐filtered ²³Na imaging of the dog head in vivo