Evaluation of multidimensional capillary electrophoretic methodologies for determination of amino bisphosphonate pharmaceuticals

Fragmentation patterns of new esterified and unesterified aromatic 1‐hydroxymethylene‐1, 1‐bisphosphonic acids by ESI‐MSn

“…Surprisingly, while a lot of new HMBP compounds have been described, only a few investigations have been undertaken to find efficient approaches to their analysis. To explain the difficulties associated with HMBP analysis, authors often evoke their polar ionic nature and the lack of ultraviolet or fluorescent‐sensitive chromophores 16–19. Chromatographic methodologies require derivatization to yield readily detectable products 18, 19.…”

Development of HPLC‐UV method for rapid and sensitive analysis of topically applied tetracaine: its comparison with a CZE method

“…This observation is in agreement with those of other authors in comparisons of the two methods (Clohs and McErlane, ; Aurora Prado et al ., ; Muscarella et al ., ; Carlucci et al ., ; Kowalski and Plenis, ; Kocevar et al ., ). Sometimes HPLC was not useful (Hilz et al ., ), owing to the physicochemical properties of the analyte resulting in poor resolution and reproducibility (Bexheti et al ., ).…”

Column Coupling Electrophoresis in Biomedical Analysis

“…The analytical benefits of the ITP-CZE combination have been already well documented (Fanali et al, 2000;Danková et al, 2001;Kvasnička et al, 2001;Valcárcel et al, 2001;Bexheti et al, 2006;Beckers, 2000b;Křivánková et al, 1991;Křivánková & Thormann, 1993;Křivánková & Boček, 1997a). An on-line combination of ITP with CZE appears to be promissing for alleviating some of the following practical problems : i. ITP is a separation technique with a well defined concentrating power while the separands migrate stacked in sharp zones, i.e., it can be considered as an ideal sample injection technique for CZE, ii.…”

“…1. In the ITP-ITP mode both preseparation (wider) and analytical (narrower) capillaries are filled with (i) the same leading electrolyte (one-dimensional ITP) or (ii) different electrolytes (two-dimensional ITP) (Flottmann et al, 2006;Bexheti et al, 2006;Mikuš et al, 2006b;Kubačák et al, 2006aKubačák et al, , 2006bKubačák et al, , 2007. The ITP separation in a concentration cascade, introduced into conventional CE by Boček et al, (Boček et al, 1978) enhances the detectabilities of the separated constituents from the response of the conductivity detection due to well-known links between the concentration of the leading electrolyte and the lengths (volumes) of the zones The first ITP stage of the ITP-ITP combination can apply all benefits as they are described for the ITP stage of the ITP-CZE combination in section 3.1.1.1.…”