Evaluation of mucoadhesive properties of chitosan microspheres prepared by different methods

Abstract

The mucoadhesive properties of chitosan microspheres prepared by different methods were evaluated by studying the interaction between mucin and microspheres in aqueous solution. The interaction was determined by the measurement of mucin adsorbed on the microspheres. A strong interaction between chitosan microspheres and mucin was detected. The intensity of the interaction was dependent upon the method of preparation of chitosan microspheres and the amount of mucin added. The extent of mucus adsorption was proportional to the absolute values of the positive zeta potential of chitosan microspheres. The zeta potential in turn was found to be dependent upon the method of preparation of microspheres. The adsorption of type III mucin (1% sialic acid content) was interpreted using Freundlich or Langmuir adsorption isotherms. The values of r 2 were greater for Langmuir isotherm as compared with Freundlich isotherm. The adsorption of a suspension of chitosan microspheres in the rat small intestine indicated that chitosan microspheres prepared by tripolyphosphate cross-linking and emulsification ionotropic gelation can be used as an excellent mucoadhesive delivery system. The microspheres prepared by glutaraldehyde and thermal cross-linking showed good stability in HCl as compared with microspheres prepared by tripolyphosphate and emulsification ionotropic gelation.

Formulation and evaluation of carvedilol microcapsules using Eudragit NE30D and sodium alginate

“…The increase in zeta potential may be due to the contribution of HPBCD and Eudragit NE30D.This increase in zeta potential of the formulation also contributes to the increase in mucoadhesive property of the formulation as seen in Table III. This result is in confirmation with earlier studies (Dhawan, Singla, Sinha , 2004).…”

Newer Trends in In Situ Gelling Systems for Controlled Ocular Drug Delivery

“…It is biocompatible, biodegradable, mucoadhesive, easily available, nontoxic, inexpensive and organic solvents are not required for its solubilization [67]. The excellent muoadhesive properties of chitosan are due to the electrostatic interaction between positively charged chitosan and negatively charged mucosal surfaces [68]. Further, it has been shown to possess permeation enhancing effect across biological surfaces.…”

The use of chitosan-6-mercaptonicotinic acid nanoparticles for oral peptide drug delivery

“…Stability of the particles in acid media was evaluated by a method described by Dhawan et al (2004) Briefly, the particles were incubated in 0.1 M HCl in a final concentration of 1% (m/v). At pre-determined time points the transmission of the samples was measured at 400 nm.…”

“…As the decrease in turbidity is directly dependent on the disintegration of the particles, transmission is used to measure the concentration of non-disintegrated particles (Dhawan et al, 2004). As shown in Figure 3, the acidic suspension of particles became more transparent over time.…”