Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study

Jefferis, Roy; Reimer, Charles B.; Skvaril, F; Lange, Gerda de; Ling, N. R.; Lowe, J; Walker, Matthew R.; Phillips, Donald J.; Aloisio, Carol H.; Wells, Thomas W.; Vaerman, Jean‐Pierre; Magnusson, C. G. M.; Kubagawa, Hiromi; Cooper, Melissa; Vartdal, Frode; Vandvik, B.; Haaijman, J.J.; Mãkelä, O.; Sarnesto, Annikki; Lando, Zeev; Gergely, J.; Rajnavölgyi, Éva; László, Glória; Rádl, J.; Molinaro, Giuseppe A.

doi:10.1016/0165-2478(85)90082-3

Cited by 341 publications

(80 citation statements)

References 14 publications

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“…Several studies indicate that the average serum proportion of the IgG subclasses is as follows: IgG1 65%, IgG2 30%, IgG3 5% and IgG4 4% [38]. IgG1 appears at constant levels in terms of systemic and local concentration and local production.…”

Section: Discussionmentioning

confidence: 99%

“…Microtitre plates (Immulon 4; Dynatech Labs, Billingshurst, UK) were coated with anti-human IgG1, IgG2, IgG3 or IgG4 (Sigma). These monoclonals have been shown to be completely specific for their subclass [38]. This was followed by incubation with a standard human serum, the IgG1-4 subclass concentration of which had been determined by nephelometry (Department of Clinical Immunology, Western Infirmary, Glasgow).…”

Section: Determination Of Igg and Iga Subclass Protein And Iga1 Fragmmentioning

confidence: 99%

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IgG and IgA subclass mRNA-bearing plasma cells in periodontitis gingival tissue and immunoglobulin levels in the gingival crevicular fluid

Takahashi

Mooney²,

Frandsen

et al. 1997

Clinical and Experimental Immunology

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SUMMARYThe humoral immune response, especially the production of IgG and IgA, is considered to have a protective role in the pathogenesis of periodontal disease, but the precise mechanisms are still unknown. In order to determine local IgG and IgA production, we investigated the presence of human IgG and IgA subclass mRNA-bearing plasma cells within periodontal tissue by in situ hybridization using digoxigenin-labelled oligonucleotide probes in 24 gingival biopsy samples (pocket depth 5 5 mm) which were obtained from eight patients with adult periodontitis. Furthermore, we examined IgG and IgA subclass proteins and digested IgA1 Fab portions in the gingival crevicular fluid (GCF), corresponding to the sites from which the tissues were taken, by ELISA. IgG and IgA subclass mRNA-expressing cells were detected in all serial formalin-fixed/paraffinembedded gingival tissue sections sampled. Plasma cells showed strong cytoplasmic staining with a high contrast and a good retention of morphology with these probes. IgG1 mRNA-expressing cells were predominant (mean 63%) and IgG2 mRNA-expressing cells were present at around 23% of total IgG plasma cells, while IgG3 and IgG4 mRNA-expressing cells were present to a much lesser extent (3% and 10%, respectively). Similar proportions of IgG subclass proteins in GCF were detected, which were also consistent with 'normal' serum levels. In terms of IgA subclass, IgA1 mRNA-positive cells were predominant (mean 65 . 1%, P

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Section: Discussionmentioning

confidence: 99%

Section: Determination Of Igg and Iga Subclass Protein And Iga1 Fragmmentioning

confidence: 99%

IgG and IgA subclass mRNA-bearing plasma cells in periodontitis gingival tissue and immunoglobulin levels in the gingival crevicular fluid

Takahashi

Mooney²,

Frandsen

et al. 1997

Clinical and Experimental Immunology

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“…The monoclonal anti-human IgG subclass antibodies and their dilution of use are shown in Table 1. The monospecificity of these subclass antibodies has been demonstrated by a World Health Organisation Collaborative Study (Jefferis et al 1985).…”

Section: Elisa Methodsmentioning

confidence: 99%

Specific IgG subclass antibody in rubella virus infections

Thomas

Morgan‐Capner

1988

Epidemiol. Infect.

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SUMMARYA solid-phase antigen enzyme-linked immunosorbent assay (ELISA) was developed for the detection of rubella-specific IgG subclasses. For rubella-specific IgG, and IgG3 sera were quantitated in arbitrary units (au) by comparison with standard curves. A concentration of 3 au was taken as that indicating positivity for specific IgG1 and specific IgG3. No sera reactive for specific IgG2 and IgG4 have been found, and thus the assay reagents were controlled by testing dilutions of a standard calibrant serum containing known concentrations of the specific IgG subclasses.Of 105 unselected sera negative for rubella antibody by radial haemolysis (RH), two gave concentrations of specific IgG, > 3 au and both were positive by rubella latex agglutination (LA).

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“…In essence, MAbs may perform well in some assay systems but may be poor or ineffective in others [1,2]. In addition, the specificity of a given reagent can also be determined by the assay system employed [3].…”

Section: Introductionmentioning

confidence: 99%

Characterisation of epitopes of pan-IgG/anti-G3m(u) and anti-Fc monoclonal antibodies

et al. 2003

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The characterisation of monoclonal antibodies (MAbs) and their epitopes is important prior to their application as molecular probes. In this study, Western blotting using IgG1 Fc and pFc? fragments was employed to screen seven MAbs before pepscan analysis to determine their reactivity to potentially linear epitopes. MAbs PNF69C, PNF110A, X1A11 and MAbs WC2, G7C, JD312, 1A1 detected epitopes within the C H 3 and C H 2 domains, respectively. However, only four MAbs showed pepscan profiles that highlighted likely target residues. In particular, MAbs PNF69C and PNF110A that have previously been characterised with pan-IgG and anti-G3m(u) specificity, detected the peptide motif 338-KAKGQPR-344 which was located within the N-terminal region of the C H 3 domain. Furthermore the majority of residues were present in all four IgG subclasses. Consequently the peptide identified was consistent with the pan-IgG nature of these antibodies. By using PCImdad, a molecular display programme, this sequence was visualised as surface accessible, located in the C H 2/C H 3 inter-domain region and proximal to the residue arginine 435 . It is speculated that this residue may be important for phenotypic expression of G3m(u) and specificity of these reagents. Pepscan analysis of MAbs G7C and JD312 (both pan-IgG) highlighted the core peptide sequence 290-KPREE-294, which was present in the C H 2 domain and was common to all four IgG subclasses. PCImdad also showed this region to be highly accessible and was consistent with previous bioinformatic and autoimmune analysis of IgG. Overall these MAbs may serve as useful anti-IgG or antiG3m(u) reagents and probes of immunoglobulin structure. #

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Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study

Cited by 341 publications

References 14 publications

IgG and IgA subclass mRNA-bearing plasma cells in periodontitis gingival tissue and immunoglobulin levels in the gingival crevicular fluid

IgG and IgA subclass mRNA-bearing plasma cells in periodontitis gingival tissue and immunoglobulin levels in the gingival crevicular fluid

Specific IgG subclass antibody in rubella virus infections

Characterisation of epitopes of pan-IgG/anti-G3m(u) and anti-Fc monoclonal antibodies

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