Evaluation of Microorganism from Integrated Processing Mixed Sheep Feces and Rice Straw as Starter Culture

Abstract

This study aims to determine the potential of microorganism in processing of mixed sheep feces and rice straw, used as a starter in the preservation of forage feed. The method is, in the 1st phase with 3 treatments of carbon and nitrogen ratio with 6 repetitions and the 2nd phase with 3 treatments of molasses in the base medium, which are grouped in dry matter content with 6 replications. The results of the 1st phase showed the preferred treatment of T2 (C/N ratio 30) yielded an initial number of bacteria of 2.87 x 1014 cfu/g and fungi 9.6 x 1013 cfu/g, and decreased into 4.2x 1010 cfu/g and 3.7 x 1011 cfu/g, respectively. At the end of initial degradation, bacteria identified were Bacillus sp and fungi identified were Aspergillus niger, Aspergillus glaucus, Aspergillus terreus, Rizophus sp. The results of the 2nd phase showed that the highest bacterial colonies were achieved in T2 with DM3 of 38 x 106 cfu / ml and the bacterial consortium identified were Bacillus sp and Lactobacillus sp. The number and types of bacterial consortium isolated and identified in the liquid medium can be used as a starter in the preservation of forage feed.