Evaluation of Microbial Spoilage of Some Aquacultured Fresh Fish in Benin City Nigeria

Abstract

A microbiological study of organisms associated with spoilage of fresh fish samples collected from two ponds located in Benin City, Nigeria was carried out. Samples from the fish skin, gills and flesh were cultured in three media; nutrient agar, Maconkay agar and potato dextrose agar and on examination the presence of five bacteria species namely; Staphylococcus aureus, Klebsiella sp., Salmonella sp., Escherichia coli, Pseudomonas sp., and four fungi species namely; Aspergillis niger, Geotrichum sp., Rhizopus sp. and Pennicillium sp. was confirmed. The highest colony count was obtained from the skin samples in all the media. Antibiotic sensitivity pattern showed that all isolates were resistant to Gentamicin and Amoxicillin and the presence of the above pathogens in the fresh fish samples could pose a potential public health threat especially to consumers. It is recommended that better handling and processing methods should be adopted to reduce or eliminate health risk to fresh fish consumers.

Screening of Bacterial and Fungal Biota Associated with Oreochromis niloticus in Lake Manzala and Its Impact on Human Health

“…coli., and potential pathogenic organisms such as Klebsiella spp., Citrobacter spp., and Proteus spp. [46] reported that isolation of some most pathogenic organisms such as Salmonella spp., E. coli and potential pathogenic organisms as Klebsiella spp., Citrobacter spp. and Proteus spp., from fish and fish products gives an indication about environmental fecal pollution of fish.…”

Antibiotic resistance profile of Pseudomonas aeruginosa isolated from aquaculture and abattoir environments in urban communities

“…[10,11]. This is a crucial issue as these organisms might migrate into the food chain resulting in food infection or directly to humans resulting in difficulties to treat infections [12].…”

“…Previous studies have isolated and characterized Pseudomonas species from poultry [14], abattoir environments [1,3,4,7,15], effluent water from a brewery [16] and fresh and dry fish species [12,17]. Abattoirs, aquaculture environment and associated wastewaters are potential sources of pathogenic bacteria that could possess, act as reservoirs and spread antibiotic resistance genes within bacterial population to food of animals as well as humans.…”

Isolation of Enterobacteriaceae From Raw Seafoods Sold in Fish Markets in Eastern Province of Saudi Arabia.

“…Some human pathogens such as Escherichia coli, Klebsiellasp, Salmonellasp, Citrobactersp and Proteus sp, when isolated from fish and fish products gives an identification about environmental fecal pollution of fish. These organisms have been found to survive and multiply in the gut, mucus and tissues of fish and that render fish acting as potential vector of human disease over long periods (Onyangoet al, 2009;Wogu and Maduakol, 2010).…”