Evaluation of methods of transport and cultivation of bacterial specimens from infected dental root canals

Preservation of Fusobacterium nucleatum and Peptostreptococcus anaerobius DNA after loss of cell viability

“…The effectiveness of air killing of both species was initially established by observation of nongrowth on FAA plates. A further high‐sensitivity test, using an enriched medium, FTM, which is favourable for recovery of low numbers of damaged cells (Carlsson & Sundqvist ), confirmed that the air‐killed cells were incapable of growth. Additional investigation of the intactness of the cell walls of air‐killed cells is discussed in more detail below.…”

“…Noncultivability was confirmed by plating 100 μL of each suspension on FAA, incubated anaerobically for 5 days (37 °C) as described above. As an additional high‐sensitivity test for cell viability, 10 μL of the bacterial suspensions was transferred to an enriched medium favourable for bacterial cell recovery, fluid thioglycollate medium (FTM; 5 mL), and incubated anaerobically (37 °C) for 14 days (Carlsson & Sundqvist ). Aliquots (1.25 mL) of each of the suspensions containing the killed cells were transferred to Microtubes (Sarstedts, Nümbrecht, Germany) and incubated aerobically for 1–6 months (37 °C).…”

Bacterial Quantification in Teeth with Apical Periodontitis Related to Instrumentation and Different Intracanal Medications: A Randomized Clinical Trial

“…It contains a small amount of agar that prevents diffusion of oxygen to the medium (28,31). This medium is equivalent or superior to pre-reduced anaerobically sterilized transport media because it allows recovery of a greater number of bacteria than other complex media (26).…”

“…Pre-reduced thioglycolate broth containing agar, dextrose, and indicator (Oxoid Ltd., Basingstoke, Hampshire, UK) was used to transfer the samples obtained in the clinic to the laboratory because of its capacity to maintain the vitality of sampled bacteria (26). Four milliliters of the medium, prepared according to the manufacturer's instructions, were dispensed into screw-cap tubes.…”

How Useful Is Root Canal Culturing in Predicting Treatment Outcome?

“…Anaerobic sampling and cultivation techniques are indispensable to analyses of MRS and they are far from straightforward. Special medium supplemented with agar is generally required to prevent oxygen diffusion so that toxic intermediates of oxygen do not accumulate and interfere with viability of anaerobic bacteria (12). Moreover, to obtain growth from potentially positive samples, the incubation time must not be less than about 2 weeks, which, from a microbiological research perspective, is costly and time-consuming (10).…”