Evaluation of meat, fruit and vegetables from retail stores in five United Kingdom regions as sources of extended-spectrum beta-lactamase (ESBL)-producing and carbapenem-resistant Escherichia coli

Randall, Luke; Lodge, M. P.; Elviss, Nicola; Lemma, Fabrizio; Hopkins, Katie L.; Teale, C. J.; Woodford, Neil

doi:10.1016/j.ijfoodmicro.2016.10.036

Cited by 85 publications

(87 citation statements)

References 34 publications

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“…In addition, ESBL- E. coli was found in 20% of pre-packaged fresh meat products. The high prevalence of ESBL- E. coli in chicken meat (16/30, 53%) is similar to previous studies conducted in the UK and the Netherlands (12, 25, 26). However, E. coli from livestock were not closely related to isolates causing human disease in our region, suggesting that livestock are not a direct source of infecting isolates and that human invasive E. coli are not being shared with livestock.…”

Section: Discussionsupporting

confidence: 89%

“…A limitation of our study is that we did not include all possible sources of E. coli for humans (for example, vegetables, fruits and pets), although a recent study found no E. coli with CTX-M-15 (the dominant human ESBL type) in retail meat, fruit and vegetables in five UK regions (25). We acknowledge that the E. coli from humans pre-dated the surveys of farms and retail meat, but we accounted for this by identifying relatedness based on a 0-15 SNP cut-off given the estimated E. coli mutation rate of 1 SNP/core genome/year (18, 19).…”

Section: Discussionmentioning

confidence: 99%

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One Health genomic surveillance ofEscherichia colidemonstrates distinct lineages and mobile genetic elements in isolates from humans versus livestock

Ludden

Raven

Jamrozy

et al. 2018

Preprint

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Running title: One Health genomic surveillance of Escherichia coli 25 2 ABSTRACT 26 Livestock have been proposed as a reservoir for drug-resistant Escherichia coli that infect 27 humans. We isolated and sequenced 431 E. coli (including 155 ESBL-producing isolates) 28 from cross-sectional surveys of livestock farms and retail meat in the East of England. These 29 were compared with the genomes of 1517 E. coli associated with bloodstream infection in the 30 United Kingdom. Phylogenetic core genome comparisons demonstrated that livestock and 31 patient isolates were genetically distinct, indicating that E. coli causing serious human 32 infection do not directly originate from livestock. By contrast, we observed highly related 33 isolates from the same animal species on different farms. Analysis of accessory (variable) 34 genomes identified a virulence cassette associated previously with cystitis and neonatal 35 meningitis that was only present in isolates from humans. Screening all 1948 isolates for 36 accessory genes encoding antibiotic resistance revealed 41 different genes present in variable 37proportions of humans and livestock isolates. We identified a low prevalence of shared 38 antimicrobial resistance genes between livestock and humans based on analysis of mobile 39 genetic elements and long-read sequencing. We conclude that in this setting, there was 40 limited evidence to support the suggestion that antimicrobial resistant pathogens that cause 41 serious infection in humans originate from livestock. 42 43 44 45 46 47 48 Importance 51The increasing prevalence of E. coli bloodstream infections is a serious public health 52 problem. We used genomic epidemiology in a One Health study conducted in the East of 53England to examine putative sources of E. coli associated with serious human disease. E. 54 coli from 1517 patients with bloodstream infection were compared with 431 isolates from 55 livestock farms and meat. Livestock-associated and bloodstream isolates were genetically 56 distinct populations based on core genome and accessory genome analyses. Identical 57 antimicrobial resistance genes were found in livestock and human isolates, but there was little 58 overlap in the mobile elements carrying these genes. In addition, a virulence cassette found in 59 humans isolates was not identified in any livestock-associated isolate. Our findings do not 60 support the idea that E. coli causing invasive disease or their resistance genes are commonly 61 acquired from livestock. 62 128 Genomic comparison of E. coli from patients with bloodstream infection, livestock, and 129 retail meat. We combined and compared the 1517 human invasive with the 431 livestock-130 associated genomes. Analysis of the 1948 genomes identified 331 multilocus sequence types 131 (STs), 44 clonal complexes (CCs) and 149 singletons (STs that did not share alleles at six out 132 of seven loci with any other STs). Most STs were host-specific, with 192 human-specific STs 133 (1261/1517 isolates, 83%), 98 livestock-specific STs (225/411 isolates, ...

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

One Health genomic surveillance ofEscherichia colidemonstrates distinct lineages and mobile genetic elements in isolates from humans versus livestock

Ludden

Raven

Jamrozy

et al. 2018

Preprint

View full text Add to dashboard Cite

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“…As revealed previously by our group (Pehlivanlar Önen et al, ), a very high rate (86.7%) of ESBL‐EC in chicken meat samples was found in the current study. This prevalence of ESBL‐EC in chicken meat is comparable to these obtained in several countries, for example; recent studies showed that contamination can vary from as low as 65.4% in England (Randall et al, ) to as high as 91.7% in France (Casella, Nogueira, Saras, Haenni, & Madec, ). It has been previously suggested that the previous use of cephalosporins, aminoglycosides and fluoroquinolones in poultry industry had created selection pressure for the emergence of high rate of ESBL carriage in chickens (Canton et al, ).…”

Section: Discussionsupporting

confidence: 82%

Evaluation of bulk tank raw milk and raw chicken meat samples as source of ESBL producing Escherichia coli in Turkey: Recent insights

Kürekçi

Osek

Aydın

et al. 2018

Journal of Food Safety

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Extended spectrum β‐lactamase producing Escherichia coli (ESBL‐EC) was detected in 86.6% of chicken and 22.6% of bulk tank milk (BTM) samples. Pulsed‐field gel electrophoresis analysis revealed 49 distinct restriction profiles among 66 isolates, and 62.3% of the isolates carried the blaCTX‐M gene, among which CTX‐M‐1 was found to be the predominant ESBL types in chicken isolates, whereas CTX‐M‐15 was the commonest among BTM samples. Additionally, of 52 ESBL‐EC isolates from chicken meat samples, 36.5%, 9.6%, and 7.7% harbored the blaTEM, blaCMY‐2, and blaSHV‐12 genes, respectively, compared with 28.5% of the blaTEM and 7.1% of the blaSHV‐12 markers among BTM isolates. The fimH gene was present in 51 isolates of chicken and in 14 isolates of BTM samples, while other virulence genes iutA (n = 31), iroN (n = 26), kpsMT II (n = 5), papC (n = 2), papG allele II (n = 2), papG allele II‐III (n = 2), and papEF (n = 2) were only present in chicken meat isolates. Overall, it can be said that contaminated chicken meat and BTM might serve as vehicles for playing potential role in zoonotic transmission of ESBL‐EC to humans in Turkey. Practical applications Extended spectrum β‐lactamase producing E. coli (ESBL‐EC) have been considered to be one of the major worldwide clinical problems and have been frequently isolated from the foods of animal origins such as chicken meat, fish, and raw milk. The current study aimed to isolate ESBL‐EC from chicken and bulk tank milk samples and further characterize the strains by PFGE, identifying resistance genes and as well as virulence genes. Given the high prevalence of CTX‐M‐15/55 type ESBL‐EC strains possessing important virulence genes in chicken and milk samples, it can be said that foods of animal origins might be an important risk factor for extraintestinal ESBL‐EC infections for humans.

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“…Is important to note that carbapenem-resistant and extendedspectrum β-lactamase (ESBL)-producing E. coli has become widespread in different reservoirs and represent an emerging public health threat (Nirupamaa et al, 2018;Pulss, Semmler, Prenger-Berninghoff, Bauerfeind, & Ewers, 2017;Randall et al, 2017). Some studies have also indicated that the transfer of ESBL-producing bacteria and/or ESBL-encoding genes to humans could happen via the food chain (Ewers, Bethe, Semmler, Guenther, & Wieler, 2012).…”

Section: Discussionmentioning

confidence: 99%

Genotypic and phenotypic characterization of Escherichia coli isolated from mollusks in Brazil and the United States

et al. 2018

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The aim of this study was to determine the serogroups, antimicrobial resistance and genetic diversity of Escherichia coli isolates from samples of bivalve mollusks collected along Santa Catarina coast, Brazil, and from the Chesapeake Bay, Maryland, USA. One hundred forty‐one E. coli isolates were characterized for serogroups with 181 specific O antisera and antimicrobial susceptibility using the disk diffusion method. The genetic diversity was assessed using pulsed‐field gel electrophoresis (PFGE). The results showed that among the isolates, 19.9% were classified as multi‐drug resistant (MDR) and resistance was most frequently observed to cephalothin, nitrofurantoin, and ampicillin. The predominant serogroups were O6, O8, and O38. Some serogroups were recognized as pathogenic E. coli . PFGE dendrograms indicated extensive genetic diversity among the isolates. Although characteristics of the E. coli isolates were highly variable, it is important to note that E. coli belonging to pathogenic serogroups and MDR isolates are present in mollusks of both study areas. This is the first report on the phenotypic and genotypic characterization of E. coli from mollusks from Santa Catarina and the Chesapeake Bay that should encourage studies focusing on comparison of isolates across countries.

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Evaluation of meat, fruit and vegetables from retail stores in five United Kingdom regions as sources of extended-spectrum beta-lactamase (ESBL)-producing and carbapenem-resistant Escherichia coli

Cited by 85 publications

References 34 publications

One Health genomic surveillance ofEscherichia colidemonstrates distinct lineages and mobile genetic elements in isolates from humans versus livestock

One Health genomic surveillance ofEscherichia colidemonstrates distinct lineages and mobile genetic elements in isolates from humans versus livestock

Evaluation of bulk tank raw milk and raw chicken meat samples as source of ESBL producing Escherichia coli in Turkey: Recent insights

Genotypic and phenotypic characterization of Escherichia coli isolated from mollusks in Brazil and the United States

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