Evaluation of mature miR398 family, expression analysis and the post-transcriptional regulation evidence in gamma-irradiated and nitrogen-stressed<i>Medicago sativa</i>seedlings

Javed, Mohammed; Sinha, Anshika; Shukla, Lata I.

doi:10.1080/09553002.2019.1558298

Cited by 13 publications

(6 citation statements)

References 57 publications

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“…2c), and overexpression of gma-miR398a did not reduce Arabidopsis drought tolerance. Collectively, these results imply that different miR398 family members may have diverse effects in legumes [9]. Therefore, overexpression and knockout assays of gma-miR398c were performed using a soybean hairy-root transformation system.…”

Section: Drought-responsive Gma-mir398c Negatively Regulated Drought Tolerance In Transgenic Plantsmentioning

confidence: 99%

“…gma-miR394 and gma-miR396, by overexpressing in model plants have been verified to perform an important function in response to drought stress [6,7]. As known, miR398 participates in stress resistance by regulating the expression of superoxide dismutase (SOD)-related genes in many plants, such as Arabidopsis thaliana [8], Medicago sativa [9], tomato [10], and Oryza sativa [11]. Overexpressing miR398 negatively regulates plant stress tolerance in Arabidopsis thaliana and Nicotiana benthamiana [12,13].…”

Section: Introductionmentioning

confidence: 99%

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Integration of sRNA, degradome, transcriptome analysis and functional investigation reveals gma-miR398c negatively regulates drought tolerance via GmCSDs and GmCCS in transgenic Arabidopsis and soybean

Zhou

Liu

et al. 2020

BMC Plant Biol

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Background Drought conditions adversely affect soybean growth, resulting in severe yield losses worldwide. Increasing experimental evidence indicates miRNAs are important post-transcriptional regulators of gene expression. However, the drought-responsive molecular mechanism underlying miRNA–mRNA interactions remains largely uncharacterized in soybean. Meanwhile, the miRNA-regulated drought response pathways based on multi-omics approaches remain elusive. Results We combined sRNA, transcriptome and degradome sequencing to elucidate the complex regulatory mechanism mediating soybean drought resistance. One-thousand transcripts from 384 target genes of 365 miRNAs, which were enriched in the peroxisome, were validated by degradome-seq. An integrated analysis showed 42 miRNA–target pairs exhibited inversely related expression profiles. Among these pairs, a strong induction of gma-miR398c as a major gene negatively regulates multiple peroxisome-related genes (GmCSD1a/b, GmCSD2a/b/c and GmCCS). Meanwhile, we detected that alternative splicing of GmCSD1a/b might affect soybean drought tolerance by bypassing gma-miR398c regulation. Overexpressing gma-miR398c in Arabidopsis thaliana L. resulted in decreased percentage germination, increased leaf water loss, and reduced survival under water deficiency, which displayed sensitivity to drought during seed germination and seedling growth. Furthermore, overexpressing gma-miR398c in soybean decreased GmCSD1a/b, GmCSD2a/b/c and GmCCS expression, which weakened the ability to scavenge O2.−, resulting in increased relative electrolyte leakage and stomatal opening compared with knockout miR398c and wild-type soybean under drought conditions. Conclusion The study indicates that gma-miR398c negatively regulates soybean drought tolerance, and provides novel insights useful for breeding programs to improve drought resistance by CRISPR technology.

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Section: Drought-responsive Gma-mir398c Negatively Regulated Drought Tolerance In Transgenic Plantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Integration of sRNA, degradome, transcriptome analysis and functional investigation reveals gma-miR398c negatively regulates drought tolerance via GmCSDs and GmCCS in transgenic Arabidopsis and soybean

Zhou

Liu

et al. 2020

BMC Plant Biol

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“…As a result of various studies on this miRNA, it was determined that the miR398 family is associated with oxidative stress mechanisms in higher plants. In addition, miR398 expression has been shown to differ between plant tissues and different stress conditions [47][48][49][50]. As a result of their transcriptome study in tomato, Feng et al [51] determined a 6-fold increase in the expression of miR398a-5p after biotic stress.…”

Section: Discussionmentioning

confidence: 99%

Expression analysis of miRNAs and their targets related to salt stress in Solanum lycopersicum H-2274

Çakır

Arıkan

Karpuz

et al. 2021

Biotechnology & Biotechnological Equipment

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The microRNAs (miRNAs) are a group of small non-coding RNAs found in both plants and animals. Their functions and target genes have been identified in many plant species. It has been shown that miRNAs have crucial roles in stress responses of plants as much as other defence mechanisms. Throughout abiotic stress, miRNAs regulate their target genes and take part in signalling pathways. In this study, we evaluated the expression profiles of miR156, miR170, miR171, miR395, miR398 and miR845, which have been shown to be related with stress, and their target genes in different tissues of Solanum lycopersicum H-2274 plants under salt stress. We applied 100 mmol/L NaCl to tomato plants for one week and performed quantitative polymerase chain reaction (qPCR) analysis. The results that stood out belonged to miR156 both in root and stem tissues and miR398 in stem tissues. Interestingly the expression profiles of these miRNAs and their target genes were similar. These findings show that there is a complex stress response mechanism still waiting to be deciphered. Our findings could provide valuable information to understand the function of miRNAs in stress response mechanisms of plants.

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“…did not reduce Arabidopsis drought tolerance. Collectively, these results imply that different miR398 family members may have diverse effects in legumes [9]. Therefore, overexpression and knockout essays of gma-miR398c were performed using a soybean hairy-root transformation system.…”

Section: Drought-responsive Gma-mir398c Negatively Regulated Drought mentioning

confidence: 99%

“…gma-miR394 and gma-miR396, by overexpressing in model plants have been verified to perform an important function in response to drought stress [6,7]. As known, miR398 participates in stress resistance by regulating the expression of superoxide dismutase (SOD)-related genes in many plants, such as Arabidopsis thaliana [8], Medicago sativa [9], potato [10], and Oryza sativa [11]. Overexpressing miR398 negatively regulates plant stress tolerance in Arabidopsis thaliana and Nicotiana benthamiana [12,13].…”

mentioning

confidence: 99%

Integration of sRNA, degradome, transcriptome analysis and functional investigation reveals gma-miR398c negatively regulates drought tolerance via GmCSDs and GmCCS in transgenic Arabidopsis and soybean

Zhou

Liu

et al. 2019

Preprint

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Background: Drought conditions adversely affect soybean growth, resulting in severe yield losses worldwide. Increasing experimental evidence indicates miRNAs are important post-transcriptional regulators of gene expression. However, the drought-responsive molecular mechanism underlying miRNA–mRNA interactions remains largely uncharacterized in soybean. Meanwhile, the miRNA-regulated drought response pathways based on multi-omics approaches remain elusive. Results: We combined sRNA, transcriptome and degradome sequencing to elucidate the complex regulatory mechanism mediating soybean drought resistance. One-thousand transcripts from 384 target genes of 365 miRNAs, which were enriched in the peroxisome, were validated by degradome-seq. An integrated analysis showed 42 miRNA–target pairs exhibited inversely related expression profiles. Among these pairs, a strong induction of gma-miR398c as a hub gene negatively regulates multiple peroxisome-related genes ( GmCSD 1a/b, GmCSD 2a/b/c and GmCCS ). Meanwhile, we detected that alternative splicing of GmCSD1a/b might affect soybean drought tolerance by bypassing gma-miR398c regulation. Overexpressing gma-miR398c in Arabidopsis thaliana L. resulted in decreased percentage germination, increased leaf water loss, and reduced survival under water deficiency, which displayed sensitivity to drought during seed germination and seedling growth. Furthermore, overexpressing gma-miR398c in soybean decreased GmCSD 1a/b, GmCSD 2a/b/c and GmCCS expression, which weakened the ability to scavenge O 2 .− , resulting in increased relative electrolyte leakage and stomatal opening compared with knockout miR398c and wild-type soybean under drought conditions. Conclusion: The study indicates that gma-miR398c negatively regulates soybean drought tolerance, and provides novel insights useful for breeding programs to improve drought resistance by CRISPR technology.

show abstract

Evaluation of mature miR398 family, expression analysis and the post-transcriptional regulation evidence in gamma-irradiated and nitrogen-stressedMedicago sativaseedlings

Cited by 13 publications

References 57 publications

Integration of sRNA, degradome, transcriptome analysis and functional investigation reveals gma-miR398c negatively regulates drought tolerance via GmCSDs and GmCCS in transgenic Arabidopsis and soybean

Integration of sRNA, degradome, transcriptome analysis and functional investigation reveals gma-miR398c negatively regulates drought tolerance via GmCSDs and GmCCS in transgenic Arabidopsis and soybean

Expression analysis of miRNAs and their targets related to salt stress in Solanum lycopersicum H-2274

Integration of sRNA, degradome, transcriptome analysis and functional investigation reveals gma-miR398c negatively regulates drought tolerance via GmCSDs and GmCCS in transgenic Arabidopsis and soybean

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