Expression analysis of antioxidant related genes may provide key information to develop plants more tolerant to abiotic and biotic stress. For this purpose, in this study, proteosome was inhibited by using MG132 proteosome inhibitor in Lycopersicon esculentum (tomato) tissues with high antioxidant content. We aimed to explain the response of gene isoforms associated with oxidative stress metabolism as a result of proteasome inhibition induced by MG132 in tomato. Gene expression levels of SLGR1, SLPhGPX, SLCAT1, SLFe-SOD, SLGPX, SLCu/Zn-SOD, SLcAPX and SLGST genes which are known to be associated with antioxidant mechanisms, were determined by real-time PCR in tomato. There was no significant change in SLPhGPX, SLCAT1, SL-Fe-SOD and SLcAPX genes in root, stem and leaf tissues. In addition, expression of SLGR1 and SLGST genes in root tissue was increased. In leaf tissue, expression of SLGPX, SLCu/Zn-SOD and SLGST genes increased significantly. There was no significant change in the expression of the genes studied in the shoot tissue. These results obtained from tomato root, stem and leaf tissues by creating proteasome inhibition will help to understand the relationship between antioxidant system and proteasome system in plants.
The microRNAs (miRNAs) are a group of small non-coding RNAs found in both plants and animals. Their functions and target genes have been identified in many plant species. It has been shown that miRNAs have crucial roles in stress responses of plants as much as other defence mechanisms. Throughout abiotic stress, miRNAs regulate their target genes and take part in signalling pathways. In this study, we evaluated the expression profiles of miR156, miR170, miR171, miR395, miR398 and miR845, which have been shown to be related with stress, and their target genes in different tissues of Solanum lycopersicum H-2274 plants under salt stress. We applied 100 mmol/L NaCl to tomato plants for one week and performed quantitative polymerase chain reaction (qPCR) analysis. The results that stood out belonged to miR156 both in root and stem tissues and miR398 in stem tissues. Interestingly the expression profiles of these miRNAs and their target genes were similar. These findings show that there is a complex stress response mechanism still waiting to be deciphered. Our findings could provide valuable information to understand the function of miRNAs in stress response mechanisms of plants.
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