Evaluation of Malus gene bank resources with German strains of Marssonina coronaria using a greenhouse-based screening method

Wöhner, Thomas; Girichev, Vadim; Radatz, Stine; Lauria-Baca, Beatrize; Scheinpflug, H.; Hanke, Magda‐Viola

doi:10.1007/s10658-018-1588-3

Cited by 8 publications

(2 citation statements)

References 24 publications

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“…Each Petri dish was moistened with 10 mL of tap water. The extraction of the inoculum and the inoculation was performed according to a method of Wöhner et al [29,30] with modifications reported in Richter et al [25].…”

Section: Plant Materials and Diplocarpon Coronariae-resistant Leaf Testmentioning

confidence: 99%

YOLO-Based Phenotyping of Apple Blotch Disease (Diplocarpon coronariae) in Genetic Resources after Artificial Inoculation

Reim,

Richter,

Leonhardt

et al. 2024

Agronomy

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Phenotyping of genetic resources is an important prerequisite for the selection of resistant varieties in breeding programs and research. Computer vision techniques have proven to be a useful tool for digital phenotyping of diseases of interest. One pathogen that is increasingly observed in Europe is Diplocarpon coronariae, which causes apple blotch disease. In this study, a high-throughput phenotyping method was established to evaluate genetic apple resources for susceptibility to D. coronariae. For this purpose, inoculation trials with D. coronariae were performed in a laboratory and images of infested leaves were taken 7, 9 and 13 days post inoculation. A pre-trained YOLOv5s model was chosen to establish the model, which was trained with an image dataset of 927 RGB images. The images had a size of 768 × 768 pixels and were divided into 738 annotated training images, 78 validation images and 111 background images without symptoms. The accuracy of symptom prediction with the trained model was 95%. These results indicate that our model can accurately and efficiently detect spots with acervuli on detached apple leaves. Object detection can therefore be used for digital phenotyping of detached leaf assays to assess the susceptibility to D. coronariae in a laboratory.

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Section: Plant Materials and Diplocarpon Coronariae-resistant Leaf Testmentioning

confidence: 99%

YOLO-Based Phenotyping of Apple Blotch Disease (Diplocarpon coronariae) in Genetic Resources after Artificial Inoculation

Reim,

Richter,

Leonhardt

et al. 2024

Agronomy

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“…In addition, apple blotch is intractable because the recent increase in the organic farming of apples worldwide requires the limited application of fungicides [9]. Additionally, the emergence of new fungicide-resistant strains in traditional apple-production areas [10] and the lack of stable resistant cultivars [9,[11][12][13][14] have led to difficulty in resistance breeding.…”

Section: Introductionmentioning

confidence: 99%

Draft genome sequence of Marssonina coronaria, causal agent of apple blotch, and comparisons with the Marssonina brunnea and Marssonina rosae genomes

2021

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Marssonina coronaria Ellis & Davis is a filamentous fungus in the class Leotiomycetes that causes apple blotch, an economically important disease of apples worldwide. Here, we sequenced the whole genome of M. coronaria strain NL1. The genome contained 50.3 Mb with 589 scaffolds and 9,622 protein-coding genes. A phylogenetic analysis using multiple loci and a whole-genome alignment revealed that M. coronaria is closely related to Marssonina rosae and Marssonina brunnea. A comparison of the three genomes revealed 90 species-specific carbohydrate-active enzymes, 19 of which showed atypical distributions, and 12 species-specific secondary metabolite biosynthetic gene clusters, two of which have the potential to synthesize products analogous to PR toxin and swainsonine, respectively. We identified 796 genes encoding for small secreted proteins in Marssonina spp., many encoding for unknown hypothetical proteins. In addition, we revealed the genetic architecture of the MAT1-1 and MAT1-2 mating-type loci of M. coronaria, as well as 16 tested isolates carrying either MAT1-1 idiomorph (3) or MAT1-2 idiomorph (13). Our results showed a series of species-specific carbohydrate-active enzyme, secondary metabolite biosynthetic gene clusters and small-secreted proteins that may be involved in the adaptation of Marssonina spp. to their distinct hosts. We also confirmed that M. coronaria possesses a heterothallic mating system and has outcrossing potential in nature.

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Evidence of apple blotch resistance in wild apple germplasm (Malus spp.) accessions

2020

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Diplocarpon coronariae causes apple blotch, which results in significant economic losses. Resistance to apple blotch in commercial apple cultivars has not been reported in recent literature. The Malus germplasm collection from the Fruit Genebank of the Julius Kühn-Institut that is maintained in Dresden, Germany consists of 516 accessions of about 49 Malus species. This apple gene pool was evaluated for resistance to Diplocarpon coronariae. A four-year field survey was subsequently followed by artificial inoculation of field-selected accessions. Fifteen accessions that represent nine different species and hybrids were confirmed to be highly resistant following a repeated inoculation assay in the laboratory. The results from this investigation is of high interest to breeders for future apple breeding programmes and investigations of resistance to Diplocarpon coronariae.

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Evaluation of Malus gene bank resources with German strains of Marssonina coronaria using a greenhouse-based screening method

Cited by 8 publications

References 24 publications

YOLO-Based Phenotyping of Apple Blotch Disease (Diplocarpon coronariae) in Genetic Resources after Artificial Inoculation

YOLO-Based Phenotyping of Apple Blotch Disease (Diplocarpon coronariae) in Genetic Resources after Artificial Inoculation

Draft genome sequence of Marssonina coronaria, causal agent of apple blotch, and comparisons with the Marssonina brunnea and Marssonina rosae genomes

Evidence of apple blotch resistance in wild apple germplasm (Malus spp.) accessions

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