Evidence of apple blotch resistance in wild apple germplasm (Malus spp.) accessions

Wöhner, Thomas; Emeriewen, Ofere Francis; Höfer, Monika

doi:10.1007/s10658-020-02156-w

Cited by 3 publications

(1 citation statement)

References 27 publications

(27 reference statements)

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“…Each Petri dish was moistened with 10 mL of tap water. The extraction of the inoculum and the inoculation was performed according to a method of Wöhner et al [29,30] with modifications reported in Richter et al [25].…”

Section: Plant Materials and Diplocarpon Coronariae-resistant Leaf Testmentioning

confidence: 99%

YOLO-Based Phenotyping of Apple Blotch Disease (Diplocarpon coronariae) in Genetic Resources after Artificial Inoculation

Reim,

Richter,

Leonhardt

et al. 2024

Agronomy

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Phenotyping of genetic resources is an important prerequisite for the selection of resistant varieties in breeding programs and research. Computer vision techniques have proven to be a useful tool for digital phenotyping of diseases of interest. One pathogen that is increasingly observed in Europe is Diplocarpon coronariae, which causes apple blotch disease. In this study, a high-throughput phenotyping method was established to evaluate genetic apple resources for susceptibility to D. coronariae. For this purpose, inoculation trials with D. coronariae were performed in a laboratory and images of infested leaves were taken 7, 9 and 13 days post inoculation. A pre-trained YOLOv5s model was chosen to establish the model, which was trained with an image dataset of 927 RGB images. The images had a size of 768 × 768 pixels and were divided into 738 annotated training images, 78 validation images and 111 background images without symptoms. The accuracy of symptom prediction with the trained model was 95%. These results indicate that our model can accurately and efficiently detect spots with acervuli on detached apple leaves. Object detection can therefore be used for digital phenotyping of detached leaf assays to assess the susceptibility to D. coronariae in a laboratory.

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Section: Plant Materials and Diplocarpon Coronariae-resistant Leaf Testmentioning

confidence: 99%

YOLO-Based Phenotyping of Apple Blotch Disease (Diplocarpon coronariae) in Genetic Resources after Artificial Inoculation

Reim,

Richter,

Leonhardt

et al. 2024

Agronomy

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Malus germplasm in Germany: collection, conservation and evaluation

Höfer,

Reim,

Peil

et al. 2023

Acta Hortic.

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Recommendations on approving the name “ Entomosporium”, with a new species, E. dichotomanthes from China (Leotiomycetes, Drepanopezizaceae)

Yang,

Jayawardena,

Zeng

et al. 2024

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The phytopathogenic genus, Entomosporium can cause serious leaf diseases worldwide. Entomosporium has long been regarded as a synonym of Diplocarpon. However, different morphologies between Entomosporium and Diplocarpon make this doubtful. Based on morpho-phylogenetic analyses, the placement of the genus was re-evaluated in this study. The combined the internal transcribed spacer gene region (ITS) and the 28S large subunit ribosomal RNA gene region (LSU) phylogenetic analysis shows that Entomosporium is an independent clade within Drepanopezizaceae and formed a sister clade to the generic type Diplocarpon. Moreover, Hymenula and Pseudopeziza do not cluster in Drepanopezizaceae. We propose to resurrect the name Entomosporium, and exclude Hymenula cerealis and Pseudopeziza medicaginis from Drepanopezizaceae and propose to treat them under Ploettnerulaceae. A new species, E. dichotomanthes is also introduced from China based on morpho-molecular analyses which is associated with Dichotomanthes tristaniicarpa.

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Evidence of apple blotch resistance in wild apple germplasm (Malus spp.) accessions

Cited by 3 publications

References 27 publications

YOLO-Based Phenotyping of Apple Blotch Disease (Diplocarpon coronariae) in Genetic Resources after Artificial Inoculation

YOLO-Based Phenotyping of Apple Blotch Disease (Diplocarpon coronariae) in Genetic Resources after Artificial Inoculation

Malus germplasm in Germany: collection, conservation and evaluation

Recommendations on approving the name “ Entomosporium”, with a new species, E. dichotomanthes from China (Leotiomycetes, Drepanopezizaceae)

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