Evaluation of Loop-Mediated Isothermal Amplification (LAMP) for Malaria Diagnosis in a Field Setting

Sirichaisinthop, Jeeraphat; Buates, Sureemas; Watanabe, Risa; Han, Eunyoung; Suktawonjaroenpon, Wachira; Krasaesub, Somporn; Takeo, Satoru; Tsuboi, Takafumi; Sattabongkot, Jetsumon

doi:10.4269/ajtmh.2011.10-0676

Cited by 77 publications

(51 citation statements)

References 16 publications

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“…The use of the simple boiling method for template preparation provides a good alternative to conventional (expensive and labor-intensive) DNA extraction methods, which might not always be possible in the field. In this study, we were able to successfully use heat-treated samples for LAMP amplification, similar to results reported by Sirichaisinthop et al, Lucchi et al, and Tao et al (28,33,34). As such, the rapid boiling method for extracting DNA from filter paper is favorable for LAMP.…”

Section: Discussionsupporting

confidence: 72%

“…The method had initially been applied in malaria diagnosis on a small scale at a field clinic in an area in Thailand where malaria is endemic by using a simple boiling method for DNA extraction to simplify template preparation (28). This preliminary study demonstrated that LAMP showed sensitivity, specificity, PPV, and NPV comparable to those of microscopy, a reference method.…”

Section: Discussionmentioning

confidence: 97%

“…The study was conducted from May 2008 to May 2011 in Maesot district, Tak province, northwestern Thailand at a walk-in malaria clinic, a government-sponsored health facility, where malaria diagnosis is based on microscopy. In this region, P. falciparum and P. vivax cause the great majority of malaria infections, but P. ovale and P. malariae have occasionally been detected (28). Study selection criteria were the following: walk-in patients aged 15 years or older, documented presence of febrile illness, and absence of evidence of severe illness.…”

Section: Methodsmentioning

confidence: 99%

“…Using heat-treated clinical samples, LAMP demonstrated sensitivity and specificity of 98.3% and 100%, respectively, compared to the gold standard of microscopy (28). In this study, we further evaluated the feasibility of our species-specific LAMP diagnostic method for malaria field diagnosis on a large scale in a resource-limited setting.…”

mentioning

confidence: 99%

“…A genus-and species-specific LAMP diagnostic method that was recently developed by our group has been applied to malaria diagnosis at a field clinic in an area where malaria is endemic in Thailand (28). Using heat-treated clinical samples, LAMP demonstrated sensitivity and specificity of 98.3% and 100%, respectively, compared to the gold standard of microscopy (28).…”

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confidence: 99%

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Loop-Mediated Isothermal Amplification Assay for Rapid Diagnosis of Malaria Infections in an Area of Endemicity in Thailand

Sattabongkot¹,

Tsuboi²,

Han³

et al. 2014

J Clin Microbiol

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The loop-mediated isothermal amplification (LAMP) method, developed by our group for diagnosis of four human malaria parasites, was evaluated on a large scale at a remote clinic in Thailand where malaria is endemic. A total of 899 febrile patients were analyzed in this study. LAMP was first evaluated in 219 patients, and the result was compared to those of two histidine-rich protein (HRP)-2 rapid diagnostic tests (RDTs) and microscopy as a gold standard. LAMP DNA extraction was conducted by a simple boiling method, and the test results were assessed visually. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were 95.7%, 100%, 100%, and 98%, respectively, for LAMP and 98.6%, 98%, 95.8%, and 99.3%, respectively, for RDTs. Since RDT-positive results were based on one out of two RDTs, the sensitivity of RDTs was slightly higher than that of LAMP. However, LAMP tended to be more specific than RDTs. LAMP next was evaluated in 680 patients, and the result was compared to that of microscopy as a gold standard. Sensitivity, specificity, PPV, NPV, and diagnostic accuracy of LAMP were 88.9%, 96.9%, 92.2%, 95.5%, and 94.6%, respectively. Nested PCR was used to confirm the discrepant results. Malaria LAMP in a remote clinic in Thailand achieved an acceptable result, indicating that LAMP malaria diagnosis is feasible in a field setting with limited technical resources. Additionally, the rapid boiling method for extracting DNA from dried blood spots proved to be simple, fast, and suitable for use in the field.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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