Sample-to-Answer Microfluidic Nucleic Acid Testing (NAT) on Lab-on-a-Disc for Malaria Detection at Point of Need

Choi, Gihoon; Guan, Weihua

doi:10.1007/978-1-0716-1803-5_16

Cited by 5 publications

(3 citation statements)

References 50 publications

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“…Therefore, the facility for the probe-based LAMP methods is more likely to be more costly than the traditional LAMP methods. It is worth noting that some portable fluorescence analyzers and isothermal fluorimeters with minimal laboratory resources are currently available or under development (Supplementary Table S1) [70][71][72][73][74][75]. Furthermore, end-point fluorescence detection requires much less instrumentation than real-time monitoring.…”

Section: Comparison Of the Probe-based Lamp Methods With The Traditio...mentioning

confidence: 99%

Evolution of the Probe-Based Loop-Mediated Isothermal Amplification (LAMP) Assays in Pathogen Detection

2023

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Loop-mediated isothermal amplification (LAMP), as the rank one alternative to a polymerase chain reaction (PCR), has been widely applied in point-of-care testing (POCT) due to its rapid, simple, and cost-effective characteristics. However, it is difficult to achieve real-time monitoring and multiplex detection with the traditional LAMP method. In addition, these approaches that use turbidimetry, sequence-independent intercalating dyes, or pH-sensitive indicators to indirectly reflect amplification can result in false-positive results if non-specific amplification occurs. To fulfill the needs of specific target detection and one-pot multiplex detection, a variety of probe-based LAMP assays have been developed. This review focuses on the principles of these assays, summarizes their applications in pathogen detection, and discusses their features and advantages over the traditional LAMP methods.

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Section: Comparison Of the Probe-based Lamp Methods With The Traditio...mentioning

confidence: 99%

Evolution of the Probe-Based Loop-Mediated Isothermal Amplification (LAMP) Assays in Pathogen Detection

2023

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“…Additionally, its long detection time makes it unsuitable for point-of-care applications. Therefore, the principles of PCR are applied in microfluidic assays [104][105][106][107]. Thermal cycling can be achieved using two typical methods: spatially based and time-based thermal cycling.…”

Section: Thermal Cyclingmentioning

confidence: 99%

Advances in Simple, Rapid, and Contamination-Free Instantaneous Nucleic Acid Devices for Pathogen Detection

Wang,

Zhou

et al. 2023

Biosensors

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Pathogenic pathogens invade the human body through various pathways, causing damage to host cells, tissues, and their functions, ultimately leading to the development of diseases and posing a threat to human health. The rapid and accurate detection of pathogenic pathogens in humans is crucial and pressing. Nucleic acid detection offers advantages such as higher sensitivity, accuracy, and specificity compared to antibody and antigen detection methods. However, conventional nucleic acid testing is time-consuming, labor-intensive, and requires sophisticated equipment and specialized medical personnel. Therefore, this review focuses on advanced nucleic acid testing systems that aim to address the issues of testing time, portability, degree of automation, and cross-contamination. These systems include extraction-free rapid nucleic acid testing, fully automated extraction, amplification, and detection, as well as fully enclosed testing and commercial nucleic acid testing equipment. Additionally, the biochemical methods used for extraction, amplification, and detection in nucleic acid testing are briefly described. We hope that this review will inspire further research and the development of more suitable extraction-free reagents and fully automated testing devices for rapid, point-of-care diagnostics.

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“…This innovation eliminates the need for thermal cycling and costly apparatus, marking substantial progress in the field. ,− Nevertheless, despite advances in developing these devices, significant limitations persist. At present, there exists no POC device capable of facilitating HIV viral load self-testing due to the following challenges: the inability to process whole blood samples, the absence of quantitative or semiquantitative capabilities within a single run, ,− and the requirement for reagents to be stored at room temperature for extended periods. These pressing issues highlight the critical need for the development of an advanced HIV viral load monitoring device, one that operates from sample to answer and is suited for point-of-need usage.…”

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confidence: 99%

Compact Point-of-Care Device for Self-Administered HIV Viral Load Tests from Whole Blood

Liu,

Politza,

Kshirsagar

et al. 2023

ACS Sens.

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Sample-to-Answer Microfluidic Nucleic Acid Testing (NAT) on Lab-on-a-Disc for Malaria Detection at Point of Need

Cited by 5 publications

References 50 publications

Evolution of the Probe-Based Loop-Mediated Isothermal Amplification (LAMP) Assays in Pathogen Detection

Evolution of the Probe-Based Loop-Mediated Isothermal Amplification (LAMP) Assays in Pathogen Detection

Advances in Simple, Rapid, and Contamination-Free Instantaneous Nucleic Acid Devices for Pathogen Detection

Compact Point-of-Care Device for Self-Administered HIV Viral Load Tests from Whole Blood

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