Evaluation of Liver Protective Activity of Moringa oleifera Bark Extract in Paracetamol Induced Hepatotoxicity in Rats

Abstract

Background: Moringa oleifera has been used in folk medicine to alleviate several diseases. In the present study, ethanolic extract of Moringa oleifera bark has been investigated to study its potential on paracetamol induced hepatotoxicity on model rats.
 Methods: Rats (150–200 gm) were divided into 5 groups containing 6 animals each. Acute hepatotoxicity was induced by paracetamol (600 mg/kg body weight) administered once daily for one week whereas the extract of investigated plant was given orally throughout the whole experiment at 250 and 500 mg/kg body weight. Silymarin (100 mg/kg body weight) was given orally as standard hepatoprotective drug. The level of hepatic injury recovery was determined by the estimation of liver enzymes like SGPT, SGOT, ALP, Bilirubin, Total protein, globulin and Albumin.
 Results: Treatment with MO extract as well as standard hepatoprotective agent silymarin ameliorated plasma levels of hepatic enzymes. Body weight was improved significantly by MO extracts (p < 0.01), whereas liver weight was recovered insignificantly. SGPT, SGOT and ALP levels were improved very highly significantly (p<0.001) and highly significantly (p<0.01) at MO 250mg dose. While at the dose of 500 mg/kg ameliorated SGPT Level very highly significantly (p<0.001), SGOT Level highly significantly (p<0.01) but insignificant to ALP level. 
 Conclusion: The biochemical parameters provide evidence that the ethanolic extract of of Moringa oleifera bark has shown hepatoprotective activity.

Hepato-Ameliorative Effect of Aqueous Extract of Moringa oleifera Stem Bark on Paracetamol-Induced Liver Injury in Wistar Rats

“…There was a slight increase in weight of liver, although not significant, when compared with positive control. This finding conforms to the study by [30] .…”

“…The results indicated a dose dependent increase in serum albumin and globulin levels which was significant when compared with paracetamol control group but not significant when compared to the control group as presented in Table 3 and figure 3. The result of the study agrees with the findings of Islam et al [30] and Omobowale et al [32] .…”

“…This reduction the levels of AST, ALT and ALP proved of the protective effect and restoration of the liver integrity which was affected by the lesion caused by paracetamol induction and this is due to the non-toxic nature and tissue protective nature against various toxic metabolites of M oleifera. The result of this study conforms to that of Islam et al [30] that carried out a similar study and opined that "M oleifera extract seemed to offer protection and maintain the functional integrity of hepatic cells." Study by Fakurazi et al [31] who carried out a study on Moringa oleifera hydroethanolic extracts effectively alleviate acetaminophen-induced hepatotoxicity in experimental rats through their antioxidant nature revealed that Administration of Moringa oleifera flowers and leaves extract significantly reduced the extent of liver damage following a high dose of acetaminophen.…”

Preventive Effects of Mandarin Fruit Peel Hydroethanolic Extract, Hesperidin, and Quercetin on Acetaminophen-Induced Hepatonephrotoxicity in Wistar Rats

“…Hepatobiliary disease was verified by the rise in AP and GT activities in conjunction with the elevation in blood total bilirubin levels [74]. According to previous publications, the pathological modification in biliary flow is what causes the rise in AP and bilirubin levels in animals treated with NAPAP [75]. It is also important to note that hyperbilirubinemia has been linked to unconjugated and conjugated bilirubin release from injured hepato-cytes, increased heme degradation, occlusion of hepatic bile ducts, and inhibition of the conjugation response [76].…”

In vitro thrombolytic activity of Moringa oleifera

“…Many pharmacological activities of M. oleifera viz. antimicrobial, anti-inflammatory, analgesic, antioxidant, anticancer, anti-urolithiasis, antiasthmatic, antipyretic, antiobesity, antihypertensive, hepatoprotective, hypoglycemic, hypolipidemic, immunomodulatory, cardiovascular, nootropic, antiulcer, etc., have been reported by several researchers (Bhattacharya et al 2014;Amjad et al 2015;Nfambi et al 2015;Helmy et al 2017;Martínez-González et al 2017;Bhattacharya et al 2018;Eremwanarue and Shittu 2018;Oboh et al 2018;Islam et al 2019;Patel and Lariya 2019;Xu et al 2019;Kilany et al 2020;Mabrouki et al 2020;Padayachee and Baijnath 2020;Ali et al 2021a;Barhoi et al 2021;Kumolosasi et al 2021;Palupi et al 2021;). It is also recommended as a poultry diet due to its high nutritional and protein content (Taufek et al 2022) and can reduce glucose in kampong chicken (Adli 2020).…”