Evaluation of latex reagents for rapid identification of Candida albicans and C. krusei colonies

Freydière, Anne-Marie; Buchaille, L; Guinet, R; Gille, Y.

doi:10.1128/jcm.35.4.877-880.1997

Cited by 34 publications

(9 citation statements)

References 36 publications

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“…For six isolates the incubation time of the ID 32C had to be prolonged up to 96 h because of low reactivity. Strains that could not unequivocally be identified by the ID 32C system were subjected to additional tests such as microscopic morphology on rice extract-Tween 80 agar (16), an agglutination test for Candida krusei (Krusei Color Fumouze, Biotrin-Trignost) (11,21), or growth at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the VITEK 2 System for Rapid Identification of Yeasts and Yeast-Like Organisms

et al. 2000

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The new VITEK 2 system is a fully automated system dedicated to the identification and susceptibility testing of microorganisms. In conjunction with the VITEK ID-YST card the VITEK 2 system allows the identification of clinically important yeasts and yeast-like organisms in 15 h due to a sensitive fluorescence-based technology. The ID-YST card consists of 47 biochemical reactions. The database comprises 51 taxa, including newly described species. In this study we evaluated the reliability of the VITEK ID-YST card for the identification of yeasts and yeast-like organisms encountered in a clinical microbiology laboratory. A total of 241 strains representing 21 species were studied. The strains were isolated from clinical samples within a period of 60 days prior to the identification. The tests were performed using 24-h to 55-h subcultures on Sabouraud-gentamicin-chloramphenicol agar. Each strain was tested in parallel using the ID 32C strip as a comparison method combined with microscopic morphology and an agglutination test for C. krusei. Overall, 222 strains (92.1%) were unequivocally identified including 11 isolates (4.6%) identified with low discrimination resolved by simple additional tests. Ten strains (4.1%) for which results were given with low discrimination could not be unequivocally identified with supplemental tests, 4 strains (1.7%) were misidentified and 5 strains (2.1%) could not be identified. In conclusion, we found that the VITEK 2 system is a rapid and accurate method for the identification of medically important yeasts and yeast-like organisms.

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Section: Methodsmentioning

confidence: 99%

Evaluation of the VITEK 2 System for Rapid Identification of Yeasts and Yeast-Like Organisms

et al. 2000

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“…However, the anti-C. dubliniensis adsorbed serum reacted with R. rubra and C. krusei, the latter showing a heterogeneous reactivity with only 10% of the cells being positive. The reactivity with the anti-C. dubliniensis adsorbed serum shown by C. krusei and R. rubra is not likely to be an important problem when identifying C. dubliniensis by indirect immunofluorescence, since R. rubra has a distinctive colony color on conventional mycological media and C. krusei can be easily differentiated from C. dubliniensis by the conventional identification methods used in the clinical microbiology laboratory such as the germ tube or Krusei color tests (6,9) as well as by growth on CHROMagar Candida medium (18).…”

Section: Discussionmentioning

confidence: 99%

Rapid Identification of Candida dubliniensis by Indirect Immunofluorescence Based on Differential Localization of Antigens on C. dubliniensis Blastospores and Candida albicans Germ Tubes

et al. 1998

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There is a clear need for the development of a rapid and reliable test for the identification of Candida dubliniensis and for the discrimination of this species from Candida albicans. In the present study we have investigated the potential use of C. dubliniensis-specific antigens as a basis for its identification. We produced an anti-C. dubliniensis serum which, after adsorption with C. albicans blastospores, was found to differentially label C. dubliniensis isolates in an indirect immunofluorescence test. In this test, the antiserum reacted with blastospores and germ tubes of C. dubliniensis and with blastospores of Candida krusei and Rhodotorula rubra but did not react with blastospores of several otherCandida species including C. albicans. The antiserum also reacted with C. albicans germ tubes. The anti-C. dubliniensis adsorbed serum reacted with specific components of 25, 28, 37, 40, 52, and 62 kDa in the C. dubliniensis extract and with a variety of antigens from other yeast species. The antigens from non-C. dubliniensis yeasts showing reactivity with the anti-C. dubliniensis adsorbed serum are mostly expressed within the cell walls of these yeast species, and this reactivity does not interfere with the use of the anti-C. dubliniensis adsorbed serum in an indirect immunofluorescence test for the rapid identification of C. dubliniensis.

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“…Isolates that were chlamydospore and/or germ tube negative were further characterized with the API 20 C AUX yeast identification panel (bioMérieux Vitek) (21). Results were read after 24, 48, and 72 h. The confirmation of C. krusei was done by agglutination with the Krusei-Color test kit (Fumouze, Levallois Perret, France) (8). All cultures identified as C. albicans were grown on SGA at 45°C to distinguish them from C. dubliniensis which, unlike C. albicans, is unable to grow at 45°C (20).…”

Section: Methodsmentioning

confidence: 99%

“…At present, the identification of Candida spp. is based on a variety of tests, including germ tube formation, production of chlamydospores, sugar and nitrate assimilation, biochemical reactions, and agglutination with specific antibodies (3,6,8). All these methods require pure cultures of the isolates and, consequently, take 24 to 48 h to complete.…”

mentioning

confidence: 99%

Membrane Filtration Test for Rapid Presumptive Differentiation of Four Candida Species

Bauters

Peleman

Moerman³

et al. 1999

J Clin Microbiol

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A rapid enzymatic two-step test for the presumptive differentiation of four Candida species commonly occurring in various clinical samples is described. The technique involves membrane filtration of a liquid sample, followed by preincubation of the membrane filter on Sabouraud glucose agar supplemented with ticarcillin-clavulanic acid to yield microcolonies. In a separate assay step, parts of the filter are placed on absorbent pads impregnated with fluorogenic 4-methylumbelliferyl (4-MU) enzyme substrates (4-MU-N-acetyl-β-d-galactosaminide, 4-MU-phosphate, 4-MU-pyrophosphate, and 4-MU-β-d-galactoside) in combination with 0.1% digitonin acting as a membrane permeabilizer. The membrane filter in contact with the assay medium is incubated to allow cleavage of the enzyme substrate, resulting in fluorescent microcolonies under long-wavelength UV light. This approach, tested on 301 clinical samples, is able to presumptively differentiate C. albicans, C. glabrata, C. krusei, and C. tropicalisand to distinguish them from other Candida spp. in about 9 to 11 h. Overall agreement with the conventional methods of 94.4% (one Candida species present in the sample) to 83.8% (multiple Candida spp. present) was obtained. The false-negative rates with reference to identification by traditional methods were 1.3% (single species) and 3.8% (multiple species).

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Evaluation of latex reagents for rapid identification of Candida albicans and C. krusei colonies

Cited by 34 publications

References 36 publications

Evaluation of the VITEK 2 System for Rapid Identification of Yeasts and Yeast-Like Organisms

Evaluation of the VITEK 2 System for Rapid Identification of Yeasts and Yeast-Like Organisms

Rapid Identification of Candida dubliniensis by Indirect Immunofluorescence Based on Differential Localization of Antigens on C. dubliniensis Blastospores and Candida albicans Germ Tubes

Membrane Filtration Test for Rapid Presumptive Differentiation of Four Candida Species

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