Evaluation of laboratory tests for dengue diagnosis in clinical specimens from consecutive patients with suspected dengue in Belo Horizonte, Brazil

Ferraz, Fernanda Oliveira; Bomfim, Maria Rosa Quaresma; Tótola, Antônio Helvécio; Ávila, Thiago Vinicius; Cisalpino, Daniel; Pessanha, José Eduardo Marques; Souza, Danielle da Glória de; Júnior, Antônio Lúcio Teixeira; Nogueira, Maurício Lacerda; Bruna-Romero, Oscar; Teixeira, Mauro Martins

doi:10.1016/j.jcv.2013.06.015

Cited by 31 publications

(27 citation statements)

References 29 publications

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“…The finding of seropositive cases in patients with clinical symptomatology in previously negative samples for titration of IgM antibodies, demonstrates the applicability of this molecular technique as an important diagnostic tool for early cases highly suspicious of dengue, in which the evolution of the disease obstructs early serological determination of IgM antibodies, similarily literature reiterates that the clinical diagnosis is not effective to differentiate dengue from other febrile conditions especially in its initial state, and that a technique such as qRT-PCR shows a high diagnostic sensitivity for this viral pathology [18][19][20].…”

Section: Discussiónmentioning

confidence: 86%

Detection of Dengue Virus in Department of Bolivar, Colombia, 2012-2013, Real-Time PCR

Gómez

García

Ochoa

et al. 2013

JBT

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Objective. To assess the presence of RNA from dengue virus by Real Time Polymerase Chain Reaction with Reverse Transcriptase (qRT-PCR), in anti-dengue IgM seronegative serum samples obtained from symptomatic patients with less than 5 days of fever, in the department of Bolivar, Colombia. Materials and Methods.Cross sectional descriptive study, where 50 anti-dengue IgM seronegative serum samples were analyzed by qRT-PCR. In addition, we reviewed the epidemiological charts of each patient in order to collect sociodemographic and blood count (CBC) data.Results. DENV-3 RNA was detected in seven (14 %) serum samples. The most common clinical manifestations were: fever (90 %; 45/50), headache (88 %; 44/50) and myalgia (74 %; 37/50). Regarding the CBC, the most significant findings were: thrombocytopenia (60 %; 30/50; 73.3% were men), and leukopenia (50 %; 25/50; 60% were men). Conclusion.Detection and typing of DENV genome by qRT-PCR could be considered a diagnostic tool for Dengue in anti-dengue IgM seronegative serum samples, especially, within four days after the onset of the signs of the disease.

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Section: Discussiónmentioning

confidence: 86%

Detection of Dengue Virus in Department of Bolivar, Colombia, 2012-2013, Real-Time PCR

Gómez

García

Ochoa

et al. 2013

JBT

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“…Many biosensors are being developed as rapidtests for dengue. They use piezoelectric, optical or electro-chemical methods [83][84].…”

Section: ]mentioning

confidence: 99%

A Review of Dengue Fever with Special Reference to Malaysia

Ahmad

Kumarasamy

Ngau

et al. 2018

AJRID

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Introduction: Dengue is the most prevalent viral mosquito-borne disease, with over 2.5 billion humans at risk given its endemicity in more than 100 countries. Globally, 50-100 million cases of dengue occur annually, with approximately 0.7% resulting in dengue haemorrhagic fever (DHF), and 22,000 deaths. In 2017, there were 83,849 reported cases of dengue fever in endemic under-reported Malaysia, with 177 deaths. One of our authors (Verasingam K 2006) here, earlier published an article in the Med J Malaysia titled “Dengue Fever in Malaysia: Time for Review?” This Article is meant as a sequel. Method: The Authors here narrate from their own personal-experiences, as well as from reviewing existing-literature. Results and Conclusion: Clinical Management has brought about vast improvements in mortality and morbidity. Similarly, great advancements in Laboratory Diagnostics. Prevention and Control methods have been desiring of greater achievements, but also show greater promise with Newer Insecticides, Innovative Methods and Vaccines. Dengue Fever would very likely become near-eradicated just like all other vaccine-preventable diseases, once comprehensive mass-vaccination programmes are available globally, using safe and very-effective tetravalent-vaccines soon to be available.

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“…Additionally, 6.5% (5/71) of the samples did not display infection with any specific serotype by PCR. This lack of amplification may be due to nonspecific amplification of DNA from another Flavivirus virus, such as East St. Louis and West Nile virus, which was mentioned by Ferraz et al (2013). In Tamaulipas, there have been reported cases of West Nile virus (Fernández-Salas et al 2007).…”

mentioning

confidence: 99%

Molecular detection of mixed infections with multiple dengue virus serotypes in suspected dengue samples in Tamaulipas, Mexico

Requena-Castro

Reyes‐López

Rodríguez-Reyna

et al. 2017

Mem. Inst. Oswaldo Cruz

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This study aimed to detect dengue virus (DENV) serotypes in serum samples obtained in Matamoros Tamaulipas, Mexico, and to determine the concordance of conventional nested reverse transcriptase polymerase chain reaction (RT-PCR) and a serological test [enzyme-linked immunosorbent assay (ELISA NS1)]. Here, we detected mixed infections consisting of four serotypes of DENV. The most prevalent serotype was DENV-1, followed by DENV-4. This is the first report of DENV-4 in our region. Mixed infections were also detected in 21.5% of samples, and the predominant coinfection consisted of DENV-1 and DENV-2. Therefore, continuous epidemiological surveillance of DENV in this area is required to predict future forms of dengue heterologous infections and the effect of this on health care.

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Evaluation of laboratory tests for dengue diagnosis in clinical specimens from consecutive patients with suspected dengue in Belo Horizonte, Brazil

Abstract: According to our results it can be suggested that lateral flow tests for NS1 detection are the most feasible methods for early diagnosis of dengue.

Cited by 31 publications

References 29 publications

Detection of Dengue Virus in Department of Bolivar, Colombia, 2012-2013, Real-Time PCR

Detection of Dengue Virus in Department of Bolivar, Colombia, 2012-2013, Real-Time PCR

A Review of Dengue Fever with Special Reference to Malaysia

Molecular detection of mixed infections with multiple dengue virus serotypes in suspected dengue samples in Tamaulipas, Mexico

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