Evaluation of L929 fibroblast attachment and proliferation on Arg-Gly-Asp-Ser (RGDS)-immobilized chitosan in serum-containing/serum-free cultures

Evaluation of RGD- or EGF-immobilized chitosan scaffolds for chondrogenic activity

“…6). Initial cell attachment occurring in the first step of cell/material interactions suggesting that the increased cell-polymer interactions play a crucial role in stimulating cell proliferation, as reported earlier by many research groups [17,18,30]. Clustered cell adhesive ligands on the surface of the 3D porous polymer scaffolds, mimicking an ECM structure in tissue, would not only increase cell-polymer interactions but might also create a favorable environment for facile cell-cell communications [23].…”

“…At 0.3 mol/ml initial RGD concentration, the immobilized amount reaches to the order of 10 −5 mol/g dry scaffold where immobilization efficiency is 63%. The following cell culture studies were all performed by using the scaffolds modified with 0.3 mM RGD which would be sufficient for cell attachment, cell spreading, clustering of integrin receptors and organization of actin stress fibers [30]. Immunostaining of EGF on chitosan scaffolds indicated the existence of EGF clearly (Fig.…”

Chitosan as a starting material for wound healing applications

“…Karakecili et al (Karakecili et al, 2007) studied cell behavior under the effect of chitosan membranes with immobilized RGD sequences. The in vitro analysis of L929 mouse fibroblasts showed that cell attachment increased with time and that the presence of RGD was critical for cell attachment, spreading and proliferation.…”

Intracellular uptake of magnetite nanoparticles conjugated with RGDS-peptide

“…12 Among the above methods, co-precipitation is a most simple, facile, and convenient way to synthesize iron oxides from aqueous Fe 2+ /Fe 3+ salt solutions by the addition of a base under inert atmosphere at room temperature. Many trials to enhance the biological function of the magnetite nanoparticles such as specific intracellular uptake, 13 organ targeting, 14 tumor specific binding 15 and cell attachment 16 have been reported. Until now, a wide variety of biological molecules such as antibodies, proteins, and targeting ligands have been bound to the surface of nanoparticles by chemical coupling via amide or ester bonds to realize the target specificity of magnetites.…”