Evaluation of RGD- or EGF-immobilized chitosan scaffolds for chondrogenic activity

Tiğli, Ragıp; Gümüşderelı́oğlu, Menemşe

doi:10.1016/j.ijbiomac.2008.04.003

Cited by 61 publications

(32 citation statements)

References 37 publications

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“…Although not discussed in detail herein, it should be noted that promising work with immobilized growth factors has also been performed in tissue engineering applications involving nerves, [26,54,61] liver, [28] pancreas, [62] and cartilage. [63][64][65][66] Table 2 provides a non-exhaustive summary of several immobilized growth factor platforms that have been used in various tissue repair and regeneration applications.…”

Section: Other Methodsmentioning

confidence: 99%

“…Dermal wound healing Polystyrene [18,22,23] PCL/PCL-PEG [14] PCL/gelatin [13] General mitogen Polystyrene [25] PMMA [99] Hepatocyte culture Glass [28,100] Cartilage repair Chitosan [64] Stem cell differentiation (neural) SAMs [90] Polystyrene [21] Stem cell differentiation (osteoblast) PMMA-g-PEG [91] Stem cell survival PMMA-g-PEG [59] Corneal repair PDMS [101,102] IGF-1 Myogenic differentiation Silicone [103] Dermal wound healing Polystyrene [22] Pancreatic stem cell differentiation Glass [62] LIF Maintenance of ESC pluripotency POMA [29] PET [104] Gelatin [88] NGF Stem cell differentiation (neural) PCL/PCL-PEG [17] Neurite extension Glass [61] pHEMA [54] Polypyrrole [26] PDGF-AA Stem cell differentiation (neural) Agarose [55] PDGF-BB Angiogenesis/Vascularization PEG [35] Demineralized bone [41] VEGF Angiogenesis/Vascularization PEG [19,33] Collagen [12,40,56] SAMs [27] PLGA [4] Gelatin [105] Stem cell differentiation (vascular/blood) Chitosan/collagen IV [10] Titanium [52] Agarose [39] a) Polymer abbreviations: PCL, poly(caprolactone); PDMS, poly(dimethyl siloxane); PEG, poly(ethylene glycol); PET, poly(ethylene terephthalate); pHEMA, poly(hydroxyethyl m...…”

Section: Egfmentioning

confidence: 99%

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Covalent Growth Factor Immobilization Strategies for Tissue Repair and Regeneration

Masters

2011

Macromolecular Bioscience

161

179

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Growth factors play a critical role in regulating processes involved in cellular differentiation and tissue regeneration, and are therefore considered essential elements in many tissue engineering strategies. The covalent immobilization of growth factors to biomaterial matrices addresses many of the challenges associated with delivering freely-diffusible growth factors and has thus emerged as a promising method of achieving localized and sustained growth factor delivery. This Feature Article discusses methods that have been used to immobilize growth factors to substrates, followed by an overview of several tissue repair and regeneration applications in which immobilized growth factors have been used.

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Section: Other Methodsmentioning

confidence: 99%

Section: Egfmentioning

confidence: 99%

Covalent Growth Factor Immobilization Strategies for Tissue Repair and Regeneration

Masters

2011

Macromolecular Bioscience

161

179

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“…Furthermore, CS-based scaffolds have also been loaded with growth factors to promote cartilage regeneration: both CS and collagen/CS/GAGs scaffolds loaded with TGF-1 were reported to promote cartilage regeneration [43,44]. CS scaffolds loaded with epidermal growth factor resulted in the promotion of chondrogenesis [45]. The CS/(N,N-dicarboxymethyl) scaffolds, loaded with BMPs, have been reported to induce the healing of articular cartilage lesion in rabbit [46].…”

Section: Chitin and Chitosan (Cs)-based Materialsmentioning

confidence: 99%

Biomimetic Polysaccharides and Derivatives for Cartilage Tissue Regeneration

Khan¹,

Ahmad²

2013

Biomimetics

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Cartilage tissue engineering is an emerging technology for the regeneration of such tissues damaged by disease or trauma. Unlike other types of tissue, cartilage does not have a blood supply and, therefore, lacks regenerative capabilities. Hence, there is an urgent need to develop cartilage tissues in clinically translatable conditions for regeneration. This fi eld of research involves the choice of the appropriate cells and biomaterials, devising signaling factors to the defect site for regeneration. The objective of this chapter is to provide a comprehensive synopsis of different approaches and recent advancements that have been taking place in this area, with an emphasis on various biomimetic polysaccharide-based biomaterials with integrated cell sources (e.g., chondrocytes, fi broblasts, and stem cells). Stem cells undergo chondrogenesis and deposit neocartilage in a variety of biomaterialbased scaffolds. However, there is still a limitation in recapitulating the properties of native tissues. Thus, the design of biomaterials that support the distribution of formed tissue is crucial for the optimization of cartilage formation. The state-ofthe art of advances in biomaterials and knowledge of their interaction with cells are also evaluated in this chapter. Additionally, the importance of signaling factors on cellular behavior that promote the production of cartilage tissue, that, in turn, mimics native tissue properties, accelerates restoration of tissue function and is clinically translatable, has been addressed here. Finally, the challenges, limitation and future prospect of cartilage regeneration are discussed.

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“…It is well known that FN is a large ECM protein that has been extensively studied because of its significant role in numerous physiological processes, such as cell adhesion, migration, proliferation, survival, and wound healing. 44,45 The availability of an FN-RGD binding site, in many cases, was sufficient to regulate cellular activities, 46,47 and offered important secondary actions, which regulate cell binding, cell signaling, cell proliferation, and differentiation. These results demonstrate that bare Fbg microfibers differ significantly in cell proliferation compared with FN/Fbg microfibers, indicating that the bare Fbg microfibers are cytocompatible, but that proliferation was not improved, as observed for FN/Fbg fibers.…”

Section: Cell-viability Studymentioning

confidence: 99%

Improved fibronectin-immobilized fibrinogen microthreads for the attachment and proliferation of fibroblasts

Rajangam¹,

An²

2013

IJN

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Abstract:The aim of this study was to fabricate fibrinogen (Fbg) microfibers with different structural characteristics for the development of 3-D tissue-engineering scaffolds. Fabricated Fbg microfibers were investigated for their biomolecule encapsulation, cell adhesion, and proliferations. Microfibers with three different concentrations of Fbg (5, 10, and 15 wt%) were prepared by a gel solvent-extraction method using a silicone rubber tube. Fbg microfibers were covalently modified with fibronectin (FN) by using water-soluble 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide as the cross-linking agent. Fbg microfibers were characterized by their FN cross-linking properties, structural morphology, and in vitro degradation. Furthermore, FN/Fbg microfibers were evaluated for cell attachment and proliferation. The biocompatibility and cell proliferation of the microfibers were assessed by measuring adenosine triphosphate activity in C2C12 fibroblast cells. Cell attachment and proliferation on microfibers were further examined using fluorescence and scanning electron microscopic images. FN loading on the microfibers was confirmed by fluorescence and infrared spectroscopy. Surface morphology was characterized by scanning electron microscopy, and showed highly aligned nanostructures for fibers made with 15 wt% Fbg, a more porous structure for fibers made with 10 wt% Fbg, and a less porous structure for those made with 5 wt% Fbg. Controlled biodegradation of the fiber was observed for 8 weeks by using an in vitro proteolytic degradation assay. Fbg microfibers with highly aligned nanostructures (15 wt%) showed enhanced biomolecule encapsulation, as well as higher cell adhesion and proliferation than another two types of FN/Fbg fibers (5 and 10 wt%) and unmodified Fbg fibers. The promising results obtained from the present study reveal that optimal structure of Fbg microfibers could be used as a potential substratum for growth factors or drug release, especially in wound healing and vascular tissue engineering, in which fibers could be applied to promote and orient cell adhesion and proliferation.

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Evaluation of RGD- or EGF-immobilized chitosan scaffolds for chondrogenic activity

Cited by 61 publications

References 37 publications

Covalent Growth Factor Immobilization Strategies for Tissue Repair and Regeneration

Covalent Growth Factor Immobilization Strategies for Tissue Repair and Regeneration

Biomimetic Polysaccharides and Derivatives for Cartilage Tissue Regeneration

Improved fibronectin-immobilized fibrinogen microthreads for the attachment and proliferation of fibroblasts

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