Keywords: yak milk, cheese, starter culture, ripening properties
IntroductionCompared with cow milk, yak milk has higher dry matter content and is richer in protein, fat, lactose, and minerals, with an abundance of colloidal and soluble calcium and phosphorus (Nikkhah, 2011). Yak milk can be a precious milk source for making cheese, given its increased accessibility in particular Asia regions. Cheese production with yak milk in Nepal has been considered as a viable commercial enterprise because of its high local demand and market value (Wiener et al., 2003). In India, yak milk is processed into low-fat paneer, with desirable health effects, such as decreasing the risk of arterial hypertension, coronary heart disease, and obesity (Kandeepan and Sangma, 2010). In China, yak milk has been traditionally used by Tibetan households to make milk tea, sour milk, butter, and "Qula," a dry and grainy "cheeselike" product made with skimmed yak milk for more than 1000 years (Tan et al., 2010). Naturally fermented yak milk is particularly popular among local herdsmen and other residents probably because of its characteristic flavor, mouth-feel, and nutritional quality (Sun et al., 2010). Although yak milk cheese production remains scarce, this cheese has been considered as a highly healthy food because of its high conjugated linoleic acid (CLA) content with a potential function in preventing heart disease, cancer, and metabolic disorders (Jiang et al., 2007;Or-Rashid et al., 2008).In cheese processing, starter cultures have important functions in acid development to hasten milk coagulation, assist in theexpulsion of whey, and influence the texture and flavor of cheese products (Law, 2001;McSweeney and Sousa, 2000). Among commercially available starter cultures, Lactococcus lactis subsp. cremoris and Lc. lactis subsp. lactis are commonly used for cheese making (Flórez et al., 2008;Lane and Fox, 1996). Although commercial starter cultures might provide optimal conditions in the different stages of cheese processing, e.g., curd formation and cheese ripening, such cultures were sometimes considered less suitable for making new types of cheese that require the use of starter culture strains with different and novel properties (Delgado and Mayo, 2004; Psoni et al., 2007). Murtaza et al. (2013) studied the sensory properties of cow and buffalo milk cheddar cheese made with both commercial and indigenous lactococcal cultures.They found that milk sources, starter cultures, and ripening temperatures significantly influenced sensory characteristics.Cheeses made with indigenous cultures and ripened at higher temperatures generally received higher sensory scores than those made with commercial cultures and ripened at lower temperatures.In this study, yak milk cheeses were made using different Analysis of cheese composition and yield Cheese samples taken at 90 d of ripening were analyzed for total protein via the Kjeldahl method (AOAC 920.123, 1990), fat via the Babcock method (AOAC 933.05, 1990), moisture via oven-drying at 102℃ (AOAC 9...