Evaluation of intraepidermal nerve fibres in the skin of normal and atopic dogs

Laprais, Aurore; Dunston, Stanley M.; Torres, Sheila M. F.; Favrot, Claude; Olivry, Thierry

doi:10.1111/vde.12420

Cited by 5 publications

(2 citation statements)

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“…Additionally, some mediators released by keratinocytes such as TSLP and periostin [ 78 ] are able to directly elicit itch by acting on sensory nerves [ 79 ]. This is particularly relevant in chronic disease [ 80 ] in which increased density of nerve fibers [ 81 ] and enhanced peripheral sensitization play a role and can contribute to a decreased response to antipruritic therapy. Thus, keratinocytes are far from being a physical inert barrier, and they are an integral part of a cross talk with the nervous system and the immune cells.…”

Section: Dogsmentioning

confidence: 99%

Atopic Dermatitis in Domestic Animals: What Our Current Understanding Is and How This Applies to Clinical Practice

Marsella

2021

Veterinary Sciences

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Atopic dermatitis is a clinical syndrome that affects both people and animals. Dogs closely mimic the complexity of the human skin disease, and much progress has been made in recent years in terms of our understanding of the role of skin impairment and the identification of new treatments. Cats and horses also develop atopic syndromes which include both cutaneous and respiratory signs, yet studies in these species are lagging. It is now recognized that atopic dermatitis is not a single disease but a multifaceted clinical syndrome with different pathways in various subgroups of patients. Appreciating this complexity is clinically relevant as we develop more targeted treatments which may work well in some patients but not in others. Different phenotypes of atopic dermatitis have been described in dogs, and it is possible that phenotypes related to breed and age may exist in other animals similar to how they are described in people. The awareness of different mechanisms of disease leads to the desire to correlate different phenotypes with specific biomarkers and responses to treatment. In this review, the current understanding and updated information on atopic syndrome in animals are described, highlighting opportunities for further studies in the future.

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Section: Dogsmentioning

confidence: 99%

Atopic Dermatitis in Domestic Animals: What Our Current Understanding Is and How This Applies to Clinical Practice

Marsella

2021

Veterinary Sciences

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“…The cells of the epidermis (keratinocytes) and the innate immune system play a critical role in AD, as shown not only in humans and rodents ( 5 ), but also in dogs ( 6 ). It has been shown that the skin of dogs with AD produces potent inflammatory mediators ( 7 ) and neurotrophins ( 8 ), which may be related to the hyperinnervation of the AD lesions ( 9 ). Pruritus, one of the most severe clinical signs of AD, is caused by a complex interface between pruritogenic molecules, keratinocytes, immunocytes, cutaneous nerve fibers, and the peripheral and central nervous systems ( 10 ).…”

Section: Introductionmentioning

confidence: 99%

Cannabinoid receptors in the inflammatory cells of canine atopic dermatitis

et al. 2022

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Materials and methods:The immunofluorescent stained cryosections of the skins of dogs with AD having antibodies against CB R, GPR , TRPV , TRPA were semiquantitatively evaluated. The inflammatory cells were identified using antibodies against tryptase (mast cells), ionized calcium binding adaptor molecule (IBA ) (macrophages/DCs), CD (T cells), and calprotectin (neutrophils). The proportions of MCs, macrophages/DCs, T cells, and neutrophils expressing CB R, GPR , TRPV and TRPA were evaluated. Results:The cells of the inflammatory infiltrate showed immunoreactivity (IR) for all or for some of the cannabinoid and cannabinoid-related receptors studied. In particular, MCs and macrophages/DCs showed CB R-, GPR -, TRPA -, and TRPV -IR; T cells showed CB R-, GPR -and TRPA -IR, and neutrophils expressed GPR -IR. Co-localization studies indicated that CB R-IR was co-expressed with TRPV -, TRPA -, and GPR -IR in di erent cellular elements of the dermis of the AD-dogs.

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Investigations on the expression and relevance of nerve growth factor in dogs with atopic dermatitis

Olivry

Bizikova

2019

Itch

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Introduction: Human atopic dermatitis (AD) keratinocytes overexpress nerve growth factor (NGF). Its inhibition, or that of its receptor, reduces itch in a mouse model of AD. In this study, we evaluated the expression of NGF in canine AD and assessed the effect of a caninized anti-NGF monoclonal antibody to delay flares of itch in dogs with natural AD. Methods: We used archived frozen skin biopsies from 6 house dust mite–sensitized atopic dogs after allergen challenge, 4 dogs with spontaneous AD and 1 dog with normal skin. The expression of NGF was evaluated by immunofluorescence. We also conducted a pilot crossover trial with 8 dogs with glucocorticoid-responsive AD. In both phases, the dogs were first treated for 28 days with oral prednisolone at 0.5 mg/kg/d. On the first day of the first phase, they received a saline subcutaneous injection, while on that of the second phase, they were injected with 0.2 mg/kg once of the caninized anti-dog NGF ranevetmab. The primary outcome measure was the time-to-flare, defined as the number of days between that of the last prednisolone administration and the day when the pruritus reached a score of at least 5.5/10, or 8 weeks, whichever came first. Results: In normal canine skin, the highest intensity of NGF staining was in stratum granulosum keratinocytes. After allergen challenge and in atopic canine skin, the NGF expression also extended downward to the upper stratum spinosum. In the pilot trial, the time-to-flare after prednisolone cessation was not significantly different between saline and ranevetmab-treated dogs. Discussion: While NGF is overexpressed in the atopic canine epidermis and after allergen challenge in sensitized dogs, the anti-NGF antibody ranevetmab did not delay pruritus flares after the discontinuation of prednisolone. Further studies are needed to assess if NGF is a relevant contributor for canine atopic itch.

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Evaluation of intraepidermal nerve fibres in the skin of normal and atopic dogs

Abstract: IENFs are detected more commonly in canine AD than in normal haired skin; these results are comparable to those seen for human AD.

Cited by 5 publications

References 50 publications

Atopic Dermatitis in Domestic Animals: What Our Current Understanding Is and How This Applies to Clinical Practice

Atopic Dermatitis in Domestic Animals: What Our Current Understanding Is and How This Applies to Clinical Practice

Cannabinoid receptors in the inflammatory cells of canine atopic dermatitis

Investigations on the expression and relevance of nerve growth factor in dogs with atopic dermatitis

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