Cannabinoid receptors in the inflammatory cells of canine atopic dermatitis

Abstract

Materials and methods:The immunofluorescent stained cryosections of the skins of dogs with AD having antibodies against CB R, GPR , TRPV , TRPA were semiquantitatively evaluated. The inflammatory cells were identified using antibodies against tryptase (mast cells), ionized calcium binding adaptor molecule (IBA ) (macrophages/DCs), CD (T cells), and calprotectin (neutrophils). The proportions of MCs, macrophages/DCs, T cells, and neutrophils expressing CB R, GPR , TRPV and TRPA were evaluated. Results:The cells of the inflammatory infiltrate showed immunoreactivity (IR) for all or for some of the cannabinoid and cannabinoid-related receptors studied. In particular, MCs and macrophages/DCs showed CB R-, GPR -, TRPA -, and TRPV -IR; T cells showed CB R-, GPR -and TRPA -IR, and neutrophils expressed GPR -IR. Co-localization studies indicated that CB R-IR was co-expressed with TRPV -, TRPA -, and GPR -IR in di erent cellular elements of the dermis of the AD-dogs.

The clinical use of cannabidiol and cannabidiolic acid–rich hemp in veterinary medicine and lessons from human medicine

“…They activate peroxisomal proliferation activation receptors in inflammatory cells to mitigate cytokine production upregulated in the skin of dogs with atopic dermatitis and other cannabinoid targets. [89][90][91] A recent placebo blinded study 92 was performed utilizing a dose of 2 mg of CBD/CBDA-rich hemp/kg every 12 hours in 17 dogs with 12 receiving placebo sesame oil for 4 weeks. Subjective veterinary measurements of inflammation using the Canine Atopic Dermatitis Scoring Index and vertical analog pruritus assessments by owners were performed, suggesting that dogs in the CBD/CBDA-rich treatment group had significant decreases in pruritus, with an average 2-point reduction and approximately 30% achieving resolution of pruritus.…”