Evaluation of intervertebral disc regeneration with implantation of bone marrow mesenchymal stem cells (BMSCs) using quantitative T2 mapping: a study in rabbits

Cai, Feng; Wu, Xiao‐Tao; Xie, Xinhui; Wang, Feng; Hong, Xin; Zhuang, Suyang; Zhu, Lei; Menemenlis, Dimitris; Shi, Rui

doi:10.1007/s00264-014-2481-0

Cited by 40 publications

(29 citation statements)

References 47 publications

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“…With increasing knowledge on stem cell transplantation, cell-based therapeutic approaches are becoming promising strategies for IVDD regeneration [5,6]. Another stem cellbased approach, which has been applied to the regeneration of many other tissues, aims to promote endogenous stem cell migration to the target area.…”

Section: Discussionmentioning

confidence: 99%

“…Bone marrow mesenchymal stem cells (BMSCs) are highly studied stem cells of different origins, including synovium, periosteum, skeletal muscle, and adipose tissues. Our previous studies demonstrated the excellent repair efficiency of BMSCs transplanted into rabbit intervertebral discs (IVDs) [6]. Besides, adiposederived stem cells have also been extensively used for repair of IVDD with the advantage of convenient availability and abundance [7], and even improve the viability of nucleus pulposus cells in degenerated intervertebral discs [8].…”

Section: Introductionmentioning

confidence: 99%

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The presence of stem cells in potential stem cell niches of the intervertebral disc region: an in vitro study on rats

et al. 2015

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To the best of our knowledge, this is the first in vitro study aimed towards determining the existence and characteristics of ISN-SCs, which belong to the MSC family and with greater osteogenic and chondrogenic abilities than BMSCs according to our data. This finding may be of great significance for additional studies that investigate the migration of ISN-SCs into the IVD, and may provide a new perspective on different biological approaches for IVD self-regeneration.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The presence of stem cells in potential stem cell niches of the intervertebral disc region: an in vitro study on rats

et al. 2015

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“…IVD degeneration is currently known as an age-related process which is accelerated by excessive mechanical loading [Guehring et al, 2010;Purmessur et al, 2013], disc injury [Yang et al, 2009;Cai et al, 2015], oxidative stress [Nasto et al, 2013], diabetes [Jiang et al, 2013], chronic tobacco smoking , obesity [Samartzis et al, 2012], and genetic inheritance [Kalichman and Hunter, 2008]. Given that the IVD mainly functions to transmit and absorb mechanical loading onto the spine, our hypothesis provides an illustrative model that depicts the biological function and disappearance of cytoplasmic vacuoles, highlighting a mechanical exhaustion of notochordal cell resources (Fig.…”

Section: Significance and Limitations Of The Hypothesismentioning

confidence: 99%

Large Cytoplasmic Vacuoles within Notochordal Nucleus Pulposus Cells: A Possible Regulator of Intracellular Pressure That Shapes the Cytoskeleton and Controls Proliferation

Hong

Zhang

Wang

et al. 2018

Cells Tissues Organs

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Degeneration of the intervertebral disc, which is closely associated with the loss of vacuolated notochordal nucleus pulposus cells (NNPC), remains a major cause of lower-back pain and motor deficiency. Being the most defining characteristic of NNPC, large cytoplasmic vacuoles not only modulate the cytoskeleton and shape cell morphology but they also respond to the disc microenvironment and regulate the biological behavior of vacuolated cells as a potent reporter of the histocytological changes that occur at the beginning of disc aging and degeneration. Here we hypothesize a model in which large cytoplasmic vacuoles primarily function to maintain a reasonable intracellular pressure (P_v) that facilitates NNPC in resisting the extracellular mechanical loading (P_e), part of which is absorbed by the extracellular matrix (P_m), forming the equation P_e = P_m + P_v. By mimicking a situation of contact-induced growth inhibition, the crowded cytoplasmic vacuoles slow down the proliferation of NNPC and restrain the generation of nonvacuolated chondrocytic nucleus pulposus cells (CNPC), whereas increased mechanical loading (↑P_e) alters cytoskeletons and breaches cytoplasmic vacuoles, which in turn weakens the vacuoles-mediated proliferation check, increases the generation of CNPC that accumulates fibrocartilaginous matrix, and rebalances the increased loading with elevated P_m (↑P_m) and lowered P_v (↓P_v), equating to ↑P_e = ↑P_m + ↓P_v. By depicting the biological function and the disappearance of the cytoplasmic vacuoles, our model highlights a mechanical exhaustion of the notochordal cell resources, which might help to elucidate the histocytological changes that initiate disc aging and degeneration.

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“…2, 3A). The results obtained by others for these two macromolecules differ between reports and largely depend on the BM-MSC growth conditions [30,48]. SOX9, responsible for the induction of type II collagen expression later during chondrogenic differentiation, is the most widely used marker in the evaluation of MSC differentiation to chondrocyte-like cells [49].…”

Section: Discussionmentioning

confidence: 93%

“…We have also investigated CD271 -MSCs and compared these cells to the entire population of MSCs generated by plastic adherence [29][30][31] …”

Section: Cd271 Mscs In Ivd Regenerationmentioning

confidence: 99%

The feasibility of the CD271+ and CD271– mesenchymal stromal cell enrichment toward nucleus pulposus-like cells

Jezierska-Woźniak

Barczewska

Habich

et al. 2017

Folia Histochem Cytobiol.

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introduction. Factors promoting nerve cell ingrowth are considered responsible for chronic back pain resulting from the intervertebral disc degeneration (IDD). One of the recent exploratory IDD treatments is stem cell transplantation therapy. The CD271 (low-affinity nerve growth factor receptor) has been identified as a marker of the most homogeneous mesenchymal stem cell (MSC) subset. It is capable of promoting differentiation along adipogenic, osteogenic and chondrogenic lineages and producing significantly higher levels of cytokines as compared to the total population of plastic adherence-mesenchymal stem cells (PA-MSCs). We investigated the ability of CD271 + MSCs to differentiate into chondrocyte-like cells of the nucleus pulposus (NP) of intervertebral disc. We also examined CD271 -MSCs, using PA-MSCs as a control cell population. Material and methods. Bone marrow derived PA-MSCs and its two subsets, CD271 -MSCs and CD271 + MSCs, were seeded in collagen scaffolds. After two weeks of growth in NP-differentiation medium, RNA was isolated from cells-scaffold constructs and was analyzed by q-PCR for expression of NP markers. Glycosaminoglycans were analyzed biochemically directly in cells-scaffold constructs. results. Expression of NP markers -extracellular matrix components such as aggrecan, collagen type II and glycosaminoglycans on both RNA and the protein levels -was significantly higher in CD271-MSCs compared to the CD271 + MSCs and PA-MSCs cell populations. Conclusions. CD271-MSCs may be superior candidates for NP restorative treatment compared to CD271 + MSCs and PA-MSCs due to their ability of expressing NP-supporting extracellular matrix components at levels higher than the other two studied MSC subsets.

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Evaluation of intervertebral disc regeneration with implantation of bone marrow mesenchymal stem cells (BMSCs) using quantitative T2 mapping: a study in rabbits

Abstract: Transplantation of BMSCs was able to promote the regeneration of degenerative discs. Quantitative and non-invasive T2 mapping could be used to evaluate the regeneration of the nucleus pulposus with good sensitivity.

Cited by 40 publications

References 47 publications

The presence of stem cells in potential stem cell niches of the intervertebral disc region: an in vitro study on rats

The presence of stem cells in potential stem cell niches of the intervertebral disc region: an in vitro study on rats

Large Cytoplasmic Vacuoles within Notochordal Nucleus Pulposus Cells: A Possible Regulator of Intracellular Pressure That Shapes the Cytoskeleton and Controls Proliferation

The feasibility of the CD271+ and CD271– mesenchymal stromal cell enrichment toward nucleus pulposus-like cells

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