The presence of stem cells in potential stem cell niches of the intervertebral disc region: an in vitro study on rats

Shi, Rui; Wang, Feng; Hong, Xin; Wang, Yuntao; Bao, Jun-Ping; Cai, Feng; Wu, Xiao‐Tao

doi:10.1007/s00586-015-4168-7

Cited by 25 publications

(23 citation statements)

References 48 publications

(71 reference statements)

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“…Our study is based on previous other studies describing the presence of stem cells derived from NP tissue [18, 19, 22, 23, 30–32]. However, studies on the cell characteristics and potential of these cells are lacking.…”

Section: Discussionmentioning

confidence: 99%

“…The detailed contents of medium A and B have been described previously [22]. First, the 2.0 × 10 4 P4 cells in each plate were incubated in induction medium A for 72 h, which was then replaced with maintenance medium B for 24 h. This 96 h induction-maintenance cycle was repeated four times and in the end, cells were incubated in maintenance medium B for 7 days.…”

Section: Methodsmentioning

confidence: 99%

“…Chondrogenic differentiation was performed as described previously [22]. Briefly, 2.0 × 10 4 P4 cells were harvested and seeded into multi-well cell culture plates in culture medium.…”

Section: Methodsmentioning

confidence: 99%

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Characteristics and potentials of stem cells derived from human degenerated nucleus pulposus: potential for regeneration of the intervertebral disc

Tang

et al. 2017

BMC Musculoskelet Disord

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BackgroundEliminating the symptoms during treatment of intervertebral disc degeneration (IVDD) is only a temporary solution that does not cure the underlying cause. A biological method to treat this disorder may be possible by the newly discovered nucleus pulposus derived stem cells (NPDCs). However, the uncertain characteristics and potential of NPDCs calls for a comprehensive study.MethodsIn the present study, nucleus pulposus samples were obtained from 5 patients with IVDD undergoing discectomy procedure and NPDCs were harvested using fluorescence activated cell sorting (FACS) by the co-expression of GD2+ and Tie2+. After in vitro expansion, the properties of NPDCs were compared with those of bone marrow mesenchyme stem cells (BMSCs) from the same subjects.ResultsNPDCs performed similar properties in cell colony-forming ability, cell proliferation rate, cell cycle and stem cell gene expression similar to those of BMSCs. In addition, NPDCs could be differentiated into osteoblasts, adipocytes, and chondrocytes, and are found to be superior in chondrogenesis but inferior in adipocyte differentiation.ConclusionsNPDCs derived from the degenerated intervertebral disc still keep the regeneration ability similar to BMSCs. Besides, the superior capacity in chondrogenesis may provide a promising cell candidate for cell-based regenerative medicine and tissue engineering in IVDD.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Characteristics and potentials of stem cells derived from human degenerated nucleus pulposus: potential for regeneration of the intervertebral disc

Tang

et al. 2017

BMC Musculoskelet Disord

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“…Animal studies suggest that the IVD contains a resident stem cell population (Shi et al, 2015; Kraus et al, 2017), however this work was performed in rodents and bovine so it is unclear if the same cell populations will be found in humans. Interestingly, the canine breed Dachshund is commonly afflicted with disc degeneration and disc herniation in a manner similar to humans (Hoffman and Dow, 2016).…”

Section: Successful Stem Cell Trials In Animalsmentioning

confidence: 99%

Implications for a Stem Cell Regenerative Medicine Based Approach to Human Intervertebral Disk Degeneration

Kraus

Lufkin

2017

Front. Cell Dev. Biol.

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The human body develops from a single cell, the zygote, the product of the maternal oocyte and the paternal spermatozoon. That 1-cell zygote embryo will divide and eventually grow into an adult human which is comprised of ~3.7 × 1013 cells. The tens of trillions of cells in the adult human can be classified into approximately 200 different highly specialized cell types that make up all of the different tissues and organs of the human body. Regenerative medicine aims to replace or restore dysfunctional cells, tissues and organs with fully functional ones. One area receiving attention is regeneration of the intervertebral discs (IVDs), which are located between the vertebrae and function to give flexibility and support load to the spine. Degenerated discs are a major cause of lower back pain. Different stem cell based regenerative medicine approaches to cure disc degeneration are now available, including using autologous mesenchymal stem cells (MSCs), induced pluripotent stem cells (iPSCs) and even attempts at direct transdifferentiation of somatic cells. Here we discuss some of the recent advances, successes, drawbacks, and the failures of the above-mentioned approaches.

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“…However, the harsh environment in IDD poses a tremendous challenge for exogenous cell survival due to limited nutrition and inflammatory conditions [22]. Several previous reports have shown that endogenous NPPCs exhibit superior potential in IDD regeneration, with better performance in the degenerated disc environment and better tissue-specific properties with lineage conservation potential [23][24][25][26]. Therefore, it is of critical importance to further elucidate these phenomena.…”

Section: Discussionmentioning

confidence: 99%

Cell properties of nucleus pulposus progenitor cells declines with intervertebral disc degeneration

Li¹,

Bai²,

Xin³

et al. 2017

Oncotarget

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Endogenous repair of nucleus pulposus progenitor cells (NPPCs) has exhibited encouraging regenerative potential for treating intervertebral disc degeneration (IDD). However, few studies have explored the properties of NPPCs during the progression of IDD. Hence, additional studies are needed to characterize NPPCs from human degenerated intervertebral disc (IVDs) at different Pfirrmann grades. In this study, human NPPCs were isolated and identified from human IVDs with different Pfirrmann grades. Then, cell biological characteristics, including proliferation, colony formation, cell senescence, migration capacity and chondrogenic ability, were compared. NPPCs were successfully harvested from grade I to IV IVDs, but not from grade V IVD due to the marked loss of nucleus pulposus tissue at grade V, leading to poor cell cultures. All four grades of NPPCs were identified as mesenchymal stem cells (MSCs) based on the criteria of the International Society for Cellular Therapy (ISCT) and shared similar cell morphological characteristics. In addition decreasing trends in proliferation, colony-formation capacity, migration, and chondrogenic ability and increasing levels of cell senescence; were detected in cells from grade I to IV IVDs, with significant changes between NPPCs of grade II and III. In summary, compared with NPPCs from normal IVDs (grade I), cells from degenerated IVDs (grades II to IV) exhibited gradually decreased cell properties and increased cell senescence. Grade II NPPCs displayed the optimal regeneration potential, suggesting that these NPPCs are an ideal candidate for endogenous repair of IDD.

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The presence of stem cells in potential stem cell niches of the intervertebral disc region: an in vitro study on rats

Cited by 25 publications

References 48 publications

Characteristics and potentials of stem cells derived from human degenerated nucleus pulposus: potential for regeneration of the intervertebral disc

Characteristics and potentials of stem cells derived from human degenerated nucleus pulposus: potential for regeneration of the intervertebral disc

Implications for a Stem Cell Regenerative Medicine Based Approach to Human Intervertebral Disk Degeneration

Cell properties of nucleus pulposus progenitor cells declines with intervertebral disc degeneration

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