Evaluation of Insulin Permeability and Effects of Absorption Enhancers on Its Permeability by an in Vitro Pulmonary Epithelial System Using Xenopus Pulmonary Membrane.

Yamamoto, Akira; Tanaka, Hideaki; Okumura, Shigeki; Shinsako, Keiko; Ito, Mayuko; Yamashita, Masahiro; Okada, Naoki; Fujita, Takuya; Muranishi, Shozo

doi:10.1248/bpb.24.385

Cited by 19 publications

(4 citation statements)

References 36 publications

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“…Interstitial eff ect of insulin due to diff usion was cited in toad urinary bladder ( Cox and Singer, 1977 ). Weak interstitial eff ect was observed in this study possibly due to diff usion, ( Sugahara et al, 1984, Yamamoto et al, 2001, Carstens et al, 1993 or because of remnants of fat tissue or blood clots ( Ribiere et al, 2002 ). Insulin is suggested to eff ect on airway smooth muscles via a NO formation pathway ( Hatzoglou et al, 2001 , Papayianni et al, 2001 ).…”

Section: Discussionmentioning

confidence: 69%

Insulin Alters the Permeability of Sheep Pleura

Vk¹,

Hatzoglou²,

Ιωάννου³

et al. 2009

Exp Clin Endocrinol Diabetes

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Section: Discussionmentioning

confidence: 69%

Insulin Alters the Permeability of Sheep Pleura

Vk¹,

Hatzoglou²,

Ιωάννου³

et al. 2009

Exp Clin Endocrinol Diabetes

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“…Interstitial effect of insulin due to diffusion was cited in toad urinary bladder [11]. Weak interstitial effect was observed in this study possibly due to diffusion [14, 15] or because of remnants of fat tissue or blood clots [16]. In kidney cells insulin is known to stimulate amiloride-sensitive Na + channels when added apically [6, 7, 11].…”

Section: Discussionmentioning

confidence: 82%

Insulin-Induced Electrophysiology Changes in Human Pleura Are Mediated via Its Receptor

Kouritas

Ιωάννου

Foroulis

et al. 2010

Experimental Diabetes Research

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Background. Insulin directly changes the sheep pleural electrophysiology. The aim of this study was to investigate whether insulin induces similar effects in human pleura, to clarify insulin receptor's involvement, and to demonstrate if glibenclamide (hypoglycemic agent) reverses this effect. Methods. Human parietal pleural specimens were mounted in Ussing chambers. Solutions containing insulin or glibenclamide and insulin with anti-insulin antibody, anti-insulin receptor antibody, and glibenclamide were used. The transmesothelial resistance (R TM) was determined. Immunohistochemistry for the presence of Insulin Receptors (IRa, IRb) was also performed. Results. Insulin increased R TM within 1st min (P = .016), when added mesothelially which was inhibited by the anti-insulin and anti-insulin receptor antibodies. Glibenclamide also eliminated the insulin-induced changes. Immunohistochemistry verified the presence of IRa and IRb. Conclusion. Insulin induces electrochemical changes in humans as in sheep via interaction with its receptor. This effect is abolished by glibenclamide.

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“…In animal studies, for instance, enhancers increased the permeability of the pulmonary membrane to inhaled insulin, but concomitant release of lactate dehydrogenase suggested some toxic effect [65].…”

Section: Delivery Systems For Inhaled Insulinmentioning

confidence: 99%

Inhaled Insulin and the Lung

Fuso¹,

Pitocco²,

Incalzi

2007

CMC

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Pulmonary delivery of insulin is more than a promise in the treatment of diabetes mellitus. Inhaled insulin seems at least as efficacious as the conventional regimen of subcutaneous insulin and/or oral glucose-lowering agents in both type 1 and type 2 diabetes mellitus. Improved metabolic control and the use of a non-invasive route of administration represent the main benefits of this new treatment. Several physico-chemical factors could reduce the bioavailability of inhaled insulin. Indeed, both deep-lung deposition and adsorption of insulin variously depend on the type of propellants used, speed of air flow, particle size and velocity, drug deposition into the throat and larger bronchial tree. These factors, in turn, depend on the pulmonary delivery systems used and on respiratory mechanics and flows. Furthermore, the pharmacokinetics of inhaled insulin is affected by smoke, which increases its absorption, and by lung diseases, which decrease the available alveolar-capillary surface. Selected abnormalities of respiratory function complicate both type 1 and type 2 diabetes mellitus and a mild depression of carbon monoxide lung transfer after a 6-month period of treatment with inhaled insulin has been reported. Finally, results from some longitudinal studies suggest that diabetes might speed up the age-related decline of lung volumes and probably alter the pharmacokinetics of inhaled insulin, particularly in the elderly. Clarifying these issues is mandatory in order to define the indications and safety of inhaled insulin.

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Evaluation of Insulin Permeability and Effects of Absorption Enhancers on Its Permeability by an in Vitro Pulmonary Epithelial System Using Xenopus Pulmonary Membrane.

Cited by 19 publications

References 36 publications

Insulin Alters the Permeability of Sheep Pleura

Insulin Alters the Permeability of Sheep Pleura

Insulin-Induced Electrophysiology Changes in Human Pleura Are Mediated via Its Receptor

Inhaled Insulin and the Lung

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