Evaluation of Insulin Like Growth Facror-1 Genetic Polymorphism with Gastric Cancer Susceptibility and Clinicopathological Features

Abstract

Gastric cancer (GC) is one of the most common malignancies in the world. It is the first cause of cancer deaths in both sexes In Iranian population. Circulating insulin-like growth factor-one (IGF-1) levels have been associated for gastric cancer. IGF-1 protein has central roles involved in the regulation of epithelial cell growth, proliferation, transformation, apoptosis and metastasis. Single nucleotide polymorphism in IGF-1 regulatory elements may lead to alter in IGF-1expression level and GC susceptibility. The aim of this study was to investigate the influence of IGF-1 gene polymorphism (rs5742612) on risk of GC and clinicopathological features for the first time in Iranian population. In total, 241 subjects including 100 patients with GC and 141 healthy controls were recruited in our study. Genotypes were analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay with DNA from peripheral blood. The polymorphism was statistically analyzed to investigate the relationship with the risk of GC and clinicopathological properties. Logistic regression analysis revealed that there was no significant association between rs5742612 and the risk of GC. In addition, no significant association between genotypes and clinicopathological features was observed (p value>0.05). The frequencies of the CC, CT, and TT genotypes were 97%, 3%, and 0%, respectively, among the cases, and 97.9%, 2.1%, and 0%, respectively, among the controls. CC genotype was more frequent in cases and controls. The frequencies of C and T alleles were 98.9% and 1.1% in controls and 98.5% and 1.5% in patient respectively. Our results provide the first evidence that this variant is rare in Iranian population and it may not be a powerful genetic predisposing biomarker for prediction GC clinicopathological features in an Iranian population.

Polymorphisms in human telomerase reverse transcriptase (hTERT) gene, gene- gene and gene-smoking interaction with susceptibility to gastric cancer in Chinese Han population

“…However, not all individuals exposed to these factors have developed GC, suggesting that genetic factors may play an important role in GC development. In recent years, some genes involved in GC risk have been reported, including microRNAs -146a, interleukin-17A (IL-17A), low molecular weight protein 2 (LMP2), LMP7, telomerase reverse transcriptase ( TERT ) and so on [ 3 – 6 ].…”

Lack of association between ERCC5 gene polymorphisms and gastric cancer risk in a Chinese population

“…However, not all individuals exposed to such risk factors develop this disease, suggesting that genetic factors may contribute to its development. Indeed, many previous studies have reported that certain genes are involved in gastric cancer risk, includingmiR-146a, IL-17A, LMP2, LMP7, IGF1, and GSTM1 (Farahani et al, 2015;Hou and Yang, 2015;Ma et al, 2015;Wei et al, 2015;Zhang and Cui, 2015).…”

Relationship between insulin-like growth factor axis gene polymorphisms and clinical outcome in advanced gastric cancer patients treated with FOLFOX

“…A Japanese study reported that two IGF1 SNPs (rs1520220 and rs2195239) were significantly associated with relapse-free survival in gastric cancer patients who had undergone curative gastrectomy [ 9 ]. In addition, an IGF1 gene polymorphism (rs5742612) was not associated with clinicopathological features in Iranian gastric cancer patients [ 18 ]. These data suggest that IGF- axis gene polymorphisms may be associated with gastric cancer progression, and that these associations may be modified by the cancer stage.…”