Evaluation of inflammatory cytokines and oxidative stress markers in prostate cancer patients undergoing curative radiotherapy

Abdel-Messeih, Phebe L; Nosseir, Neveen M.; Bakhe, Osama H

doi:10.5114/ceji.2017.67319

Cited by 12 publications

(8 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the mRNA and protein expressions of SERPINB2 in CNE2R cells in our study were determined to be significantly lower than those in CNE2 cells, which was consistent with the findings of Li XH et al [22]. Mounting data has shown that radiotherapy can induce the release of inflammatory cytokines and oxidative stress markers, and these mediators can lead to the significant upregulation of SERPINB2 in multiple cells [35, 36]. More importantly, evidence has confirmed a high correlation between SERPINB2 and EMT alteration in cells; for example, the absence of SERPINB2 enhanced cell migration via regulating EMT in tumor cells, as indicated by Longhin E et al [37].…”

Section: Discussionsupporting

confidence: 91%

SERPINB2 overexpression inhibited cell proliferation, invasion and migration, led to G2/M arrest, and increased radiosensitivity in nasopharyngeal carcinoma cells

Zhang

Wang

et al. 2019

Journal of Radiation Research

View full text Add to dashboard Cite

The aim of this study was to evaluate the effect of SERPINB2 on cell proliferation, cell cycle, epithelial–mesenchymal transition (EMT), invasion, migration, and radiosensitivity in nasopharyngeal carcinoma cells. Both CNE2R and CNE2 cells were transfected with pEGFP-N1-SERPINB2. Cell proliferation was measured by MTT assay, cell cycle by flow cytometry, and SERpINB2 expression by quantitative real-time polymerase chain reaction (qRT-PCR). Western blot was carried out to detect the protein expression. In addition, SERPINB2 and β-catenin were located intracellularly using an immunofluorescent assay, and cell migration and invasion were measured by wound healing and Transwell assays, respectively. Radiosensitivity was assessed using colony formation and MTT assays. SERPINB2 expression was downregulated in CNE2R cells. After transfection with pEGFP-N1-SERPINB2, the OD values were decreased, and there was an increased fraction in the G2/M phase. Moreover, SERPINB2 overexpression could inhibit the invasion and migration capabilities of CNE2R and CNE2 cells, with downregulation of vimentin, N-cadherin, nuclear β-catenin, matrix metalloproteinase (MMP)-2 and MMP-9, and upregulation of E-cadherin. Moreover, transfection with the SERPINB2 plasmid reduced the growth rate of CNE2R cells at doses of 2, 4 and 6 Gy, and also decreased the surviving fractions. Overexpression of SERPINB2 could reduce the proliferation, invasion and migration capabilities of CNE2R and CNE2 cells, and led to G2/M arrest via EMT inhibition, and this may be a potential strategy for enhancing the radiation sensitivity of nasopharyngeal carcinoma cells.

show abstract

Section: Discussionsupporting

confidence: 91%

SERPINB2 overexpression inhibited cell proliferation, invasion and migration, led to G2/M arrest, and increased radiosensitivity in nasopharyngeal carcinoma cells

Zhang

Wang

et al. 2019

Journal of Radiation Research

View full text Add to dashboard Cite

show abstract

“…IL-4 is a pleiotropic cytokine that is mainly produced by activated Th2 cells, mast cells, and basophils. It has been shown that the levels of IL-4 increases post-irradiation in patients with prostate cancer, and has been implicated in radiation-induced fibrosis [ 75 , 76 ]. Similar to our results, cereal fiber has been shown to decrease the levels of IL-4 in the sera of healthy persons [ 73 ].…”

Section: Discussionmentioning

confidence: 99%

Dietary Oat Bran Reduces Systemic Inflammation in Mice Subjected to Pelvic Irradiation

et al. 2020

View full text Add to dashboard Cite

Patients undergoing radiotherapy to treat pelvic-organ cancer are commonly advised to follow a restricted fiber diet. However, reducing dietary fiber may promote gastrointestinal inflammation, eventually leading to deteriorated intestinal health. The goal of this study was to evaluate the influence of dietary fiber on radiation-induced inflammation. C57BL/6J male mice were fed a High-oat bran diet (15% fiber) or a No-fiber diet (0% fiber) and were either irradiated (32 Gy delivered in four fractions) to the colorectal region or only sedated (controls). The dietary intervention started at 2 weeks before irradiation and lasted for 1, 6, and 18 weeks after irradiation, at which time points mice were sacrificed and their serum samples were assayed for 23 cytokines and chemokines. Our analyses show that irradiation increased the serum cytokine levels at all the time points analyzed. The No-fiber irradiated mice had significantly higher levels of pro-inflammatory cytokines than the High-oat irradiated mice at all time points. The results indicate that a fiber-rich oat bran diet reduces the intensity of radiation-induced inflammation, both at an early and late stage. Based on the results, it seems that the advice to follow a low-fiber diet during radiotherapy may increase the risk of decreased intestinal health in cancer survivors.

show abstract

“…This highly unpredictable mechanism renders the treatment less effective and frequently causes recurrence after radical RT—up to one-third of PCa patients experience biochemical failure after RT [ 2 ]. Despite identifying several molecular mechanisms associated with radioresistance, the ability to predict an individual PCa patient’s response to RT is still limited and based mostly on the pretreatment assessment of prostate specific antigen (PSA), tumor stage and Gleason score [ 3 , 4 ]. Therefore, there is a need to identify new biomarkers that could more accurately predict response to RT.…”

Section: Introductionmentioning

confidence: 99%

Serum Paraoxonase-1 Activity and the Risk of Prostate Cancer Recurrence in Patients Treated with Radiotherapy

Jasiński

Olszewska−Słonina

2022

Antioxidants

View full text Add to dashboard Cite

The antioxidant paraoxonase-1 (PON1) may be involved in the response to radiation-induced oxidative stress and possibly prevent cell apoptosis. The correlation of PON1 with the risk of cancer recurrence after radiotherapy (RT) is not yet explored. We investigated changes in the activity of PON1 in patients with prostate cancer (PCa) undergoing RT, and the relation of PON1 activity to the risk of recurrence after RT. We included 56 men with PCa. Blood samples were obtained before irradiation and after the completion of RT. Patients were followed for an average of 51.2 months. Each case of biochemical recurrence was confirmed with biopsy. The control group was composed of 60 healthy men. There was no significant difference in PON1 activity between the control group and patients pre-radiotherapy. Irradiation was associated with a significant decrease in PON1 activity. Patients with PCa recurrence had significantly higher serum PON1 activity than those recurrence-free, both before and after RT. PON1 activity was a predictor of PCa recurrence, with sensitivity over 80% and specificity over 64%. Our results suggest that PON1 activity may be a predictor of PCa recurrence risk after RT. Studies with a larger number of patients and longer follow-up are needed to confirm this hypothesis.

show abstract

Evaluation of inflammatory cytokines and oxidative stress markers in prostate cancer patients undergoing curative radiotherapy

Cited by 12 publications

References 24 publications

SERPINB2 overexpression inhibited cell proliferation, invasion and migration, led to G2/M arrest, and increased radiosensitivity in nasopharyngeal carcinoma cells

SERPINB2 overexpression inhibited cell proliferation, invasion and migration, led to G2/M arrest, and increased radiosensitivity in nasopharyngeal carcinoma cells

Dietary Oat Bran Reduces Systemic Inflammation in Mice Subjected to Pelvic Irradiation

Serum Paraoxonase-1 Activity and the Risk of Prostate Cancer Recurrence in Patients Treated with Radiotherapy

Contact Info

Product

Resources

About