The assessment of groundwater is essential for the estimation of suitability of water for safe use. An attempt has been made to study the groundwater at the district level of Punjab, Pakistan. These samples were analyzed for various water quality parameters like pH, color, odor, conductance, total suspended solids, trace metals (Fe, cu, B, Ba, Al, Cr, Cd, Ni, Mn and Se), ionic concentration (HCO 3 , CO 3 , Cl, SO 4 , Na, K, Ca, Mg, NO 3 , NO 2 , NH 4 , F, PO 4 and CN) and for microbiological enumeration (total viable count, total and fecal coliforms Escherichia coli, Salmonella spp. and Pseudomonas spp.). The data was analyzed with WHO guidelines/ recommendations. The results of physical analysis indicated that all samples are safe except the groundwater of Kasur and Khanewal District. About 66.67% samples are out of total suspended solids (TSS) limit. Microbiologically, only six groundwater of Punjab districts are found potable according to WHO limits. In the trace metals analysis, highest level of iron was detected in Jhang while the groundwater of three districts were not potable due to high level of boron and nickel but the groundwater of all districts was found safe with respect to Ba, Al and Cr. As far as ionic concentration is concerned, 11 districts were found not to be within WHO limit in the case of bicarbonates, seven in phosphates, five in floride, and eight in calcium and potassium. Only 10 districts fall within the limit of chlorine and all samples showed satisfactory results in the case of CN, CO 3 , NO 2 , NO 3 , Mg, SO 4 and NH 4.